Journal of Economic Entomology,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 16, 2024
Abstract
Point
mutations
in
the
acetylcholinesterase-1
gene
(ace-1)
have
been
associated
with
resistance
to
OPs
many
insects.
However,
presence
and
function
of
ace-1
OP
Helicoverpa
armigera
(Lepidoptera:
Noctuidae),
a
significant
lepidopteran
pest
damaging
wide
range
crops,
remain
largely
unexplored.
This
study
investigated
insecticide
phoxim
12
field
populations
H.
from
northern
China
2022,
revealing
low
levels
(2.5-
6.7-fold).
Using
an
amplicon
sequencing
approach,
we
screened
for
13,874
moths
collected
114
between
2006
2022.
We
found
3
amino
acid
substitutions
(A201S,
G227E,
F290V)
potentially
related
resistance.
The
mean
frequencies
A201S,
F290V
were
0.0032,
0.0001,
respectively.
To
assess
these
mutations’
role
resistance,
expressed
wild-type
mutant
AChE1
proteins
Sf9
cells.
Biochemical
characterization
revealed
3.1-fold
3.3-fold
increase
I50
chlorpyrifos-oxon
A201S
mutants
compared
enzyme,
correlating
2.9-fold
2.7-fold
decrease
Ki
value.
No
enzyme
activity
was
observed
G227E
mutant,
indicating
that
only
confer
insensitivity
chlorpyrifos-oxon.
Our
demonstrates
is
effective
method
large-scale
screening
resistance-associated
point
other
insect
pests.
It
also
identifies
as
potential
conferring
armigera.
Agronomy,
Год журнала:
2025,
Номер
15(1), С. 155 - 155
Опубликована: Янв. 10, 2025
Genetically
modified
crops
that
produce
insecticidal
proteins
from
Bacillus
thuringiensis
(Bt)
are
currently
the
most
efficient
and
safest
method
of
pest
control
worldwide.
However,
prolonged
planting
period
has
led
to
a
reduction
in
efficacy
Bt
due
evolution
resistance
field.
This
review
paper
examines
status
lepidopteran
pests
under
field
conditions,
elucidates
molecular
mechanism
underlying
their
Cry
toxins,
discusses
management
strategies
based
on
these
mechanisms.
Extensive
research
demonstrated
mutations
alterations
expression
patterns
midgut
receptor
genes
closely
associated
with
resistance.
As
our
understanding
mechanisms
progresses,
several
innovative
approaches
such
as
DNA
detection
techniques,
engineering
combining
toxin
RNAi
technology
have
been
developed
for
effective
measures.
Future
will
further
unravel
intricate
this
phenomenon
develop
scientifically
sound
integrated
strategies.
Journal of Cotton Research,
Год журнала:
2024,
Номер
7(1)
Опубликована: Сен. 3, 2024
Abstract
Cotton
plays
a
crucial
role
in
shaping
Indian
economy
and
rural
livelihoods.
The
cotton
crop
is
prone
to
numerous
insect
pests,
necessitating
insecticidal
application,
which
increases
production
costs.
advent
of
the
expression
Bacillus
thuringiensis
(Bt)
protein
has
significantly
reduced
burden
pest
without
compromising
environmental
or
human
health.
After
introduction
transgenic
cotton,
cultivated
area
expanded
22
million
hectares,
with
64%
increase
adoption
by
farmers
worldwide.
Currently,
Bt
accounts
for
93%
India.
However,
extensive
use
accelerated
resistance
development
pests
like
pink
bollworm.
Furthermore,
overreliance
on
broad-spectrum
pesticides,
favouring
emergence
secondary
significant
challenges.
This
emphasizes
urgent
necessity
developing
novel
management
strategies.
high-dose
refuge
strategy
was
initially
effective
managing
but
its
implementation
India
faced
challenges
due
misunderstandings
about
non-Bt
crops.
Although
gene
pyramiding
introduced
as
solution,
combining
mono
toxin
also
led
instances
cross-resistance.
Therefore,
there
need
further
exploration
biotechnological
approaches
manage
cotton.
Advanced
strategies,
such
sterile
release,
RNA
interference
(RNAi)-mediated
silencing,
stacking
RNAi,
genome
editing
using
clustered
regularly
interspaced
short
palindromic
repeats/CRISPR-associated
(CRISPR-Cas),
offer
promising
tools
identifying
genes
insects.
Additionally,
CRISPR-mediated
drives
biopesticides
present
potential
avenues
cultivation.
These
innovative
could
enhance
sustainability
efficacy
Journal of Economic Entomology,
Год журнала:
2024,
Номер
117(3), С. 1106 - 1112
Опубликована: Апрель 11, 2024
Abstract
Transgenic
crops
producing
Bacillus
thuringiensis
(Bt)
toxins
are
commonly
used
for
controlling
insect
pests.
Nearby
refuges
of
non-Bt
host
plants
play
a
central
role
in
delaying
the
evolution
resistance
to
Bt
by
Pervasive
fitness
costs
associated
with
resistance,
which
entail
lower
resistant
than
susceptible
individuals
refuges,
can
increase
ability
delay
resistance.
Moreover,
these
affected
environmental
factors
such
as
plant
suitability,
implying
that
manipulating
refuge
suitability
could
improve
success
strategy.
Based
on
results
from
previous
study
Trichoplusia
ni
sprays,
it
was
proposed
low-suitability
magnify
costs.
To
test
this
hypothesis,
we
investigated
association
between
and
80
observations
30
cases
reported
18
studies
8
pest
species
5
countries.
Consistent
cost
negative.
With
scaled
range
0
(low)
1
(high),
expected
20.7%
increased
2.5%
each
0.1
decrease
suitability.
The
most
common
type
involves
mutations
affecting
few
types
midgut
proteins
bind
kill
insects.
A
better
understanding
how
interact
generate
be
useful
enhancing
strategy
sustaining
efficacy
crops.
Journal of Economic Entomology,
Год журнала:
2024,
Номер
117(5), С. 2093 - 2099
Опубликована: Авг. 26, 2024
Abstract
The
cotton
bollworm,
Helicoverpa
armigera
(Hübner)
(Lepidoptera:
Noctuidae),
is
a
significant
pest
worldwide.
Bacillus
thuringiensis
(Bt)
producing
Cry1Ac
has
been
used
since
1997
for
the
control
of
this
in
China
and
increase
H.
resistance
to
occurred
northern
China.
To
mitigate
evolution,
it
necessary
develop
plant
pyramided
2-
3-toxin
Bt
replace
cotton.
For
sustainable
use
cotton,
we
diet
overlay
bioassays
measure
baseline
susceptibility
Cry2Ab
33
populations
collected
2017,
2018,
2021
12
locations
from
major
cotton-producing
areas
lethal
concentration
killing
50%
(LC50)
or
99%
(LC99)
individuals
ranged
0.030
0.138
µg/cm2
0.365
2.964
µg/cm2,
respectively.
ratio
LC50
most
resistant
susceptible
population
was
4.6,
indicating
moderate
among-population
variability
resistance.
did
not
vary
significantly
over
years.
A
diagnostic
2
calculated
as
twice
LC99
an
analysis
pooled
data
field-collected
populations.
This
discriminated
well
between
individuals,
killed
all
larvae
laboratory
strain
0%,
23%
3
strains
with
>
100-fold
Cry2Ab.
These
will
be
useful
monitoring
evolution
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(46), С. 25533 - 25548
Опубликована: Ноя. 7, 2024
Hübner
(Lepidoptera:
Noctuidae)
is
a
globally
devastating
pest
and
has
evolved
with
varying
levels
of
resistance
to
broader
spectrum
insecticides.
CarEs
are
major
enzymes
involved
in
insecticide
detoxification
metabolic
resistance.
Four
