bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 27, 2024

Summary Over 320 million years of evolution, amniotes have developed complex brains and cognition through largely unexplored genetic gene expression mechanisms. We created a comprehensive single-cell atlas over 1.3 cells from the telencephalon cerebellum turtles, zebra finches, pigeons, mice, macaques, employing resolution spatial transcriptomics to validate patterns across species. Our study revealed significant species-specific variations in cell types, highlighting their conservation diversification evolution. found pronounced differences excitatory neurons (EX) cerebellar types between birds mammals. Birds predominantly express SLC17A6 EX, whereas mammals expressed SLC17A7 neocortex elsewhere, possibly due loss function birds. Additionally, we identified novel bird-specific Purkinje subtype (SVIL+), implicating LSD11/KDM1A pathway learning circadian rhythms, related numerous positively selected genes birds, suggesting an evolutionary optimization functions for ecological behavioral adaptation. findings elucidate interplay evolution environmental adaptation, underscoring role development specialized amniotes.

Язык: Английский