ACS Synthetic Biology, Год журнала: 2025, Номер unknown

Опубликована: Май 22, 2025

CRISPR technology has revolutionized genome editing by enabling precise, permanent modifications to genetic material. To circumvent the irreversible alterations associated with traditional methods and facilitate research on both essential nonessential genes, interference or inhibition (CRISPRi) activation (CRISPRa) were developed. The gene-silencing approach leverages an inactivated Cas effector protein paired guide RNA obstruct transcription initiation elongation, while gene-activation exploits programmability of activate gene expression. Recent advances in CRISPRi technology, combination other technologies (e.g., biosensing, sequencing), have significantly expanded its applications, allowing for genome-wide high-throughput screening (HTS) identify determinants phenotypes. These strategies been applied biomedicine, industry, basic research. This review explores regulation mechanisms, offers overview workflow CRISPR-based screens, highlights superior suitability HTS across biomedical industrial applications. Finally, we discuss limitations current CRISPRi/a envision future directions CRISPR-mediated research, considering potential broader application diverse fields.

Язык: Английский