Establishment of bovine extraembryonic endoderm cells DOI Creative Commons
Hao Ming,

Giovanna Scatolin,

Alejandro Ojeda

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Understanding the mechanisms of hypoblast development and its role in implantation is critical for improving farm animal reproduction, but it hampered by lack research models. Here we report that a chemical cocktail (FGF4, BMP4, IL-6, XAV939, A83-01) enables de novo derivation long-term culture bovine extraembryonic endoderm cells (bXENs). Transcriptomic epigenomic analyses confirmed identity bXENs revealed they are resemble lineages early peri-implantation embryos. We showed help maintain stemness ESCs prevent them from differentiation. In presence signaling sustaining bXENs, growth progression epiblasts also facilitated developing pre-implantation embryo. Furthermore, through 3D assembly with TSCs, developed an improved blastocyst like structure (bovine blastoid) resembles blastocyst. The XENs blastoids established this study represent accessible vitro models understanding reproductive efficiency livestock species.

Язык: Английский

Single-Cell Genomics in Veterinary Science: Methods and Applications DOI
Byeonghwi Lim, C.K. Lim, Seung-Hoon Lee

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Gene expression changes in blastocyst hatching affect embryo implantation success in mice DOI Creative Commons
Liyou An, Liang Zhang, Yulin Wu

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Фев. 6, 2025

In mammalian embryonic development, blastocyst hatching is essential for normal implantation and development of the fetus. We reported previously that blastocysts out zona pellucida (ZP) exhibited site preferences were associated with pregnancy outcomes. To characterize these differences, we analyzed transcriptomes in following developing mouse within 16 h hatching: expanding (E), from A-site (A), B-site (B), C-site (C), hatched (H), non-hatching (N). By principal component analysis hierarchical cluster analysis, determined gene expression profiles A B blastocysts, which resulted good fertility, clustered closely. C N poor closely, but distantly B. Embryos at B- vs. C-sites, pregnancy, showed 178 differentially expressed genes (DEGs), mainly involved immunity, correlated positively birth rate. These DEGs primarily regulated by transcription factors TCF24 DLX3. During hatching, immune-related regulated, such as Ptgs1, Lyz2, Il-α, Cfb (upregulated) Cd36 (downregulated). immunofluorescence staining, found C3 IL-1β on extra-luminal surface trophectoderm blastocyst, suggesting they play a role maternal-fetal interactions. As developed to fully state, 307 either upregulated factor ATOH8 or downregulated SPIC switch immune pathways. Based outcome, identified three patterns complex changes transcriptional regulation network failed successfully blastocysts. LASSO regression-based model using Cd36, Cfb, Cyp17a1 predict success. This study revealed diverse, multidimensional developmental fates during short-term indicated properties embryo had major effect suggest their preimplantation affect implantation. contributes our understanding

Язык: Английский

Процитировано

0
Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation DOI Creative Commons
Hao Ming, Giovanna Nascimento Scatolin, Oscar Alejandro Ojeda-Rojas

и другие.

Cell Reports, Год журнала: 2025, Номер 44(6), С. 115707 - 115707

Опубликована: Май 20, 2025

Язык: Английский

Процитировано

0
Single-Cell Sequencing Technology in Ruminant Livestock: Challenges and Opportunities DOI Creative Commons

Avery Lyons,

J. J. Brown,

Kimberly M Davenport

и другие.

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(6), С. 5291 - 5306

Опубликована: Май 27, 2024

Advancements in single-cell sequencing have transformed the genomics field by allowing researchers to delve into intricate cellular heterogeneity within tissues at greater resolution. While omics are more widely applied model organisms and humans, their use livestock species is just beginning. Studies cattle, sheep, goats already leveraged single-nuclei RNA-seq as well ATAC-seq delineate diversity tissues, track changes cell populations gene expression over developmental stages, characterize immune important for disease resistance resilience. Although challenges exist of this technology ruminant livestock, such precise annotation unique spatial resolution cells a tissue, there vast potential enhance our understanding molecular mechanisms underpinning traits essential healthy productive livestock. This review intends highlight insights gained from published studies goats, particularly those with publicly accessible data. Further, manuscript will discuss opportunities how it may contribute enhanced profitability sustainability animal agriculture future.

Язык: Английский

Процитировано

2
Understanding bovine embryo elongation: a transcriptomic study of trophoblastic vesicles DOI Creative Commons
Séverine A. Degrelle, Fulin Liu, Dénis Laloë

и другие.

Frontiers in Physiology, Год журнала: 2024, Номер 15

Опубликована: Янв. 29, 2024

Background: During the process of elongation, embryo increases in size within uterus, while extra-embryonic tissues (EETs) develop and differentiate preparation for implantation. As it grows, ovoid transforms into a tubular form first then filamentous form. This is directed by numerous genes pathways, expression which may be altered case developmental irregularities such as when conceptus shorter than expected or develops after splitting. In bovines, efforts to understand molecular basis elongation have employed trophoblastic vesicles (TVs)—short EET pieces that lack an embryo—which also elongate vivo . To date, however, we analyses TVs at stages might shed light on changes involved. Methods: Following development, collected bovine conceptuses from (D12) (D18), sectioned them small with without their embryonic disc (ED), then, transferred receptive uterus assess abilities. We grew spherical blastocysts vitro up D8 subjected same treatment. Then, assessed differences gene between different samples fully elongating controls using array (10 K) extended qPCR comprising 224 across 24 pathways. Results: , elongated more less depending stage they had been created time spent utero Their daily rates differed control EET, sometimes resembling those earlier-stage EET. Overall, signatures followed similar trajectory intact D12–D18. However, each stage, displayed distinct dynamics, some were shared other short epithelial models. Conclusion: Differences likely result multiple factors, including reduction length signaling capabilities TVs, delayed inadequate uterine signals, modified crosstalk uterus. These findings confirm close coordination uterine, embryonic, required orchestrate proper and, based partial differentiation observed, raise questions about presence/absence certain cues even asynchronies.

Язык: Английский

Процитировано

1
Establishment of bovine extraembryonic endoderm cells DOI Creative Commons
Hao Ming,

Giovanna Scatolin,

Alejandro Ojeda

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Understanding the mechanisms of hypoblast development and its role in implantation is critical for improving farm animal reproduction, but it hampered by lack research models. Here we report that a chemical cocktail (FGF4, BMP4, IL-6, XAV939, A83-01) enables de novo derivation long-term culture bovine extraembryonic endoderm cells (bXENs). Transcriptomic epigenomic analyses confirmed identity bXENs revealed they are resemble lineages early peri-implantation embryos. We showed help maintain stemness ESCs prevent them from differentiation. In presence signaling sustaining bXENs, growth progression epiblasts also facilitated developing pre-implantation embryo. Furthermore, through 3D assembly with TSCs, developed an improved blastocyst like structure (bovine blastoid) resembles blastocyst. The XENs blastoids established this study represent accessible vitro models understanding reproductive efficiency livestock species.

Язык: Английский

Процитировано

0