The conserved nature of nitrogen recycling within the bacterial pouch ofTetraponeraants
Mingjie Ma,
Bi-Ru Zhu,
Dayong Zhang
и другие.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 15, 2025
Abstract
Microbial
symbioses
are
fundamental
to
the
nutrition
of
many
animal
groups,
yet
mechanisms
nutrient
recycling
have
only
been
characterized
in
a
few
social
insect
systems.
Here,
we
investigate
functional
significance
specialized
and
unusual
symbiotic
organ—the
bacterial
pouch—in
four
ant
species
within
Tetraponera
nigra
-group,
focusing
on
symbionts’
roles
nitrogen
colony
fitness.
This
unique
organ
houses
microbial
community
consistently
dominated
by
co-diversifying
symbiont
Tokpelaia
,
occasionally
accompanied
other
stable
associates.
Metagenomic
sequencing
targeted
¹⁵N-labeled
urea
feeding
experiments
demonstrate
that
these
symbionts
assimilate
from
(likely
derived
uric
acid)
transfer
it
adult
workers
developing
larvae,
incorporating
recycled
into
host
tissues.
Disruption
this
symbiosis
severely
impairs
larval
growth
overall
Overall,
our
study
highlights
critical
role
pouch
sustaining
fitness
nitrogen-limited
environments,
providing
new
insights
co-evolutionary
dynamics
between
hosts
their
partners.
Язык: Английский
Microbial bases of herbivory in beetles
Trends in Microbiology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 1, 2024
Язык: Английский
Frequent and asymmetric cell division in endosymbiotic bacteria of cockroaches
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 18, 2024
ABSTRACT
Many
insects
are
obligatorily
associated
with
and
dependent
on
specific
microbial
species
as
essential
mutualistic
partners.
In
the
host
insects,
such
mutualists
usually
maintained
in
specialized
cells
or
organs,
called
bacteriocytes
symbiotic
organs.
Hence,
potentially
exponential
growth
cannot
be
realized
but
must
strongly
constrained
by
spatial
resource
limitations
within
tissues.
How
endosymbiotic
bacteria
grow,
divide
proliferate
is
important
for
understanding
interactions
dynamics
underpinning
intimate
host-microbe
associations.
Here
we
report
that
Blattabacterium
,
ancient
endosymbiont
of
cockroaches,
exhibits
unexpectedly
high
rates
cell
division
(20-58%)
and,
addition,
asymmetric
(average
asymmetry
index
>
1.5)
when
isolated
from
German
cockroach
Blattella
germanica
.
The
at
frequencies
was
observed
irrespective
tissues
(fat
bodies
vs.
ovaries)
developmental
stages
(adults
nymphs
embryos)
B.
also
several
different
species.
By
contrast,
frequent
neither
Buchnera
obligatory
bacterial
aphids,
nor
Pantoea
gut
symbiont
stinkbugs.
Comparative
genomics
division-related
genes
uncovered
genome
lacks
Min
system
determine
plane,
which
may
relevant
to
division.
These
observations
combined
comparative
provide
insight
into
what
processes
regulations
underpin
growth,
proliferation
continuously
under
within-host
conditions.
IMPORTANCE
Diverse
their
survival
reproduction.
Due
long-lasting
coevolutionary
history,
tend
exhibit
degenerative
genomes
suffer
uncultivability.
Because
microbiological
fastidiousness,
patterns
uncultivable
have
been
poorly
described.
Here,
using
fine
microscopic
quantitative
morphometric
approaches,
that,
although
proceeds
through
symmetric
binary
fission,
Such
peculiar
were
not
other
aphids
Gene
repertoire
analysis
revealed
molecular
machineries
regulating
plane
lost
genome,
suggesting
possibility
general
trend
toward
reductive
evolution
bizarre
cytological/morphological
traits.
Язык: Английский
Frequent and asymmetric cell division in endosymbiotic bacteria of cockroaches
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 18, 2024
ABSTRACT
Many
insects
are
obligatorily
associated
with
and
dependent
on
specific
microbial
species
as
essential
mutualistic
partners.
In
the
host
insects,
such
mutualists
usually
maintained
in
specialized
cells
or
organs,
called
bacteriocytes
symbiotic
organs.
Hence,
potentially
exponential
growth
cannot
be
realized
but
must
strongly
constrained
by
spatial
resource
limitations
within
tissues.
How
endosymbiotic
bacteria
grow,
divide,
proliferate
is
important
for
understanding
interactions
dynamics
underpinning
intimate
host-microbe
associations.
Here
we
report
that
Blattabacterium
,
ancient
endosymbiont
of
cockroaches,
exhibits
unexpectedly
high
rates
cell
division
(20%–58%)
and,
addition,
asymmetric
(average
asymmetry
index
>1.5)
when
isolated
from
German
cockroach
Blattella
germanica
.
The
at
frequencies
was
observed
irrespective
tissues
(fat
bodies
vs
ovaries)
developmental
stages
(adults
nymphs
embryos)
B.
also
several
different
species.
By
contrast,
frequent
neither
Buchnera
obligatory
bacterial
aphids,
nor
Pantoea
gut
symbiont
stinkbugs.
Comparative
genomics
division-related
genes
uncovered
genome
lacks
Min
system
determine
plane,
which
may
relevant
to
division.
These
observations
combined
comparative
provide
insight
into
what
processes
regulations
underpin
growth,
division,
proliferation
continuously
under
within-host
conditions.
IMPORTANCE
Diverse
their
survival
reproduction.
Due
long-lasting
coevolutionary
history,
tend
exhibit
degenerative
genomes
suffer
uncultivability.
Because
microbiological
fastidiousness,
patterns
uncultivable
have
been
poorly
described.
Here,
using
fine
microscopic
quantitative
morphometric
approaches,
that,
although
proceeds
through
symmetric
binary
fission,
Such
peculiar
were
not
other
aphids
Gene
repertoire
analysis
revealed
molecular
machinery
regulating
plane
lost
genome,
suggesting
possibility
general
trend
toward
reductive
evolution
bizarre
cytological/morphological
traits.
Язык: Английский