Frequent and asymmetric cell division in endosymbiotic bacteria of cockroaches DOI

Tomohito Noda,

Masaki Mizutani, Toshiyuki Harumoto

и другие.

Applied and Environmental Microbiology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 18, 2024

ABSTRACT Many insects are obligatorily associated with and dependent on specific microbial species as essential mutualistic partners. In the host insects, such mutualists usually maintained in specialized cells or organs, called bacteriocytes symbiotic organs. Hence, potentially exponential growth cannot be realized but must strongly constrained by spatial resource limitations within tissues. How endosymbiotic bacteria grow, divide, proliferate is important for understanding interactions dynamics underpinning intimate host-microbe associations. Here we report that Blattabacterium , ancient endosymbiont of cockroaches, exhibits unexpectedly high rates cell division (20%–58%) and, addition, asymmetric (average asymmetry index >1.5) when isolated from German cockroach Blattella germanica . The at frequencies was observed irrespective tissues (fat bodies vs ovaries) developmental stages (adults nymphs embryos) B. also several different species. By contrast, frequent neither Buchnera obligatory bacterial aphids, nor Pantoea gut symbiont stinkbugs. Comparative genomics division-related genes uncovered genome lacks Min system determine plane, which may relevant to division. These observations combined comparative provide insight into what processes regulations underpin growth, division, proliferation continuously under within-host conditions. IMPORTANCE Diverse their survival reproduction. Due long-lasting coevolutionary history, tend exhibit degenerative genomes suffer uncultivability. Because microbiological fastidiousness, patterns uncultivable have been poorly described. Here, using fine microscopic quantitative morphometric approaches, that, although proceeds through symmetric binary fission, Such peculiar were not other aphids Gene repertoire analysis revealed molecular machinery regulating plane lost genome, suggesting possibility general trend toward reductive evolution bizarre cytological/morphological traits.

Язык: Английский

The conserved nature of nitrogen recycling within the bacterial pouch ofTetraponeraants DOI Creative Commons

Mingjie Ma,

Bi-Ru Zhu,

Dayong Zhang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 15, 2025

Abstract Microbial symbioses are fundamental to the nutrition of many animal groups, yet mechanisms nutrient recycling have only been characterized in a few social insect systems. Here, we investigate functional significance specialized and unusual symbiotic organ—the bacterial pouch—in four ant species within Tetraponera nigra -group, focusing on symbionts’ roles nitrogen colony fitness. This unique organ houses microbial community consistently dominated by co-diversifying symbiont Tokpelaia , occasionally accompanied other stable associates. Metagenomic sequencing targeted ¹⁵N-labeled urea feeding experiments demonstrate that these symbionts assimilate from (likely derived uric acid) transfer it adult workers developing larvae, incorporating recycled into host tissues. Disruption this symbiosis severely impairs larval growth overall Overall, our study highlights critical role pouch sustaining fitness nitrogen-limited environments, providing new insights co-evolutionary dynamics between hosts their partners.

Язык: Английский

Процитировано

0
Microbial bases of herbivory in beetles DOI Creative Commons
Marleny García-Lozano, Hassan Salem

Trends in Microbiology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

1
Frequent and asymmetric cell division in endosymbiotic bacteria of cockroaches DOI Creative Commons

Tomohito Noda,

Masaki Mizutani, Toshiyuki Harumoto

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 18, 2024

ABSTRACT Many insects are obligatorily associated with and dependent on specific microbial species as essential mutualistic partners. In the host insects, such mutualists usually maintained in specialized cells or organs, called bacteriocytes symbiotic organs. Hence, potentially exponential growth cannot be realized but must strongly constrained by spatial resource limitations within tissues. How endosymbiotic bacteria grow, divide proliferate is important for understanding interactions dynamics underpinning intimate host-microbe associations. Here we report that Blattabacterium , ancient endosymbiont of cockroaches, exhibits unexpectedly high rates cell division (20-58%) and, addition, asymmetric (average asymmetry index > 1.5) when isolated from German cockroach Blattella germanica . The at frequencies was observed irrespective tissues (fat bodies vs. ovaries) developmental stages (adults nymphs embryos) B. also several different species. By contrast, frequent neither Buchnera obligatory bacterial aphids, nor Pantoea gut symbiont stinkbugs. Comparative genomics division-related genes uncovered genome lacks Min system determine plane, which may relevant to division. These observations combined comparative provide insight into what processes regulations underpin growth, proliferation continuously under within-host conditions. IMPORTANCE Diverse their survival reproduction. Due long-lasting coevolutionary history, tend exhibit degenerative genomes suffer uncultivability. Because microbiological fastidiousness, patterns uncultivable have been poorly described. Here, using fine microscopic quantitative morphometric approaches, that, although proceeds through symmetric binary fission, Such peculiar were not other aphids Gene repertoire analysis revealed molecular machineries regulating plane lost genome, suggesting possibility general trend toward reductive evolution bizarre cytological/morphological traits.

Язык: Английский

Процитировано

0
Frequent and asymmetric cell division in endosymbiotic bacteria of cockroaches DOI

Tomohito Noda,

Masaki Mizutani, Toshiyuki Harumoto

и другие.

Applied and Environmental Microbiology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 18, 2024

ABSTRACT Many insects are obligatorily associated with and dependent on specific microbial species as essential mutualistic partners. In the host insects, such mutualists usually maintained in specialized cells or organs, called bacteriocytes symbiotic organs. Hence, potentially exponential growth cannot be realized but must strongly constrained by spatial resource limitations within tissues. How endosymbiotic bacteria grow, divide, proliferate is important for understanding interactions dynamics underpinning intimate host-microbe associations. Here we report that Blattabacterium , ancient endosymbiont of cockroaches, exhibits unexpectedly high rates cell division (20%–58%) and, addition, asymmetric (average asymmetry index >1.5) when isolated from German cockroach Blattella germanica . The at frequencies was observed irrespective tissues (fat bodies vs ovaries) developmental stages (adults nymphs embryos) B. also several different species. By contrast, frequent neither Buchnera obligatory bacterial aphids, nor Pantoea gut symbiont stinkbugs. Comparative genomics division-related genes uncovered genome lacks Min system determine plane, which may relevant to division. These observations combined comparative provide insight into what processes regulations underpin growth, division, proliferation continuously under within-host conditions. IMPORTANCE Diverse their survival reproduction. Due long-lasting coevolutionary history, tend exhibit degenerative genomes suffer uncultivability. Because microbiological fastidiousness, patterns uncultivable have been poorly described. Here, using fine microscopic quantitative morphometric approaches, that, although proceeds through symmetric binary fission, Such peculiar were not other aphids Gene repertoire analysis revealed molecular machinery regulating plane lost genome, suggesting possibility general trend toward reductive evolution bizarre cytological/morphological traits.

Язык: Английский

Процитировано

0