Chinese Journal of Plastic and Reconstructive Surgery, Год журнала: 2024, Номер unknown
Опубликована: Сен. 1, 2024
Опубликована: Янв. 1, 2025
White adipose tissue (WAT) is essential for energy storage and metabolic homeostasis, plays a central role in the pathology of obesity type 2 diabetes. Vascular networks within WAT support oxygen nutrient supply regulate adipogenesis, yet mechanisms underlying endothelial-adipocyte crosstalk under both healthy dysfunctional conditions remain unclear. Here, we present vascularized organoid (vAO) model engineered from human adipose-derived stem cells induced pluripotent cell-derived endothelial (SC-ECs). The 3D cell culture enables adipogenesis angiogenesis controlled conditions. vAOs demonstrated enhanced β-adrenergic-induced lipolysis insulin-stimulated glucose uptake. Single-cell transcriptomics revealed distinct stromal subtypes, including perivascular adipocyte precursors maturing SC-ECs shaped by PDGFB signaling. knockdown disrupted alignment lipolysis, highlighting critical endothelial-pericyte interactions. This study provides versatile platform to dissect explore pathways that control WAT-remodeling determine its function. Our vAO therefore presents unique molecularly functionally patient-specific dysfunctions, advancing our portfolio personalized therapies importance communication.
Язык: Английский
Процитировано
0
Biomolecules, Год журнала: 2025, Номер 15(2), С. 199 - 199
Опубликована: Янв. 30, 2025
Stromal Vascular Fraction (SVF) has gained significant attention in clinical applications due to its regenerative and anti-inflammatory properties. Initially identified decades ago, SVF is derived from adipose tissue been increasingly utilized a variety of therapeutic settings. The isolation processing protocols for have evolved substantially, particularly after classification as an Advanced Therapy Medicinal Product (ATMP), which mandates adherence Good Manufacturing Practices ensure sterility product quality. Despite the progress, few studies over last decade focused on standardization processing. Recent advances, driven by potential products such Adipose-derived Stem Cells, prompted development improved strategies aimed at enhancing their efficacy. Notable progress includes advent automated systems, reduce technical errors, minimize variability, improve reproducibility across laboratories. These developments, along with establishment more precise guidelines, enhanced consistency applicability SVF-based therapies. This review discusses key aspects processing, highlighting efforts standardize procedure reliability use.
Язык: Английский
Процитировано
0
Brain Hemorrhages, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Biomedicine & Pharmacotherapy, Год журнала: 2025, Номер 186, С. 118036 - 118036
Опубликована: Апрель 8, 2025
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Май 7, 2025
Obesity is a major global health challenge, contributing to metabolic disorders such as type 2 diabetes, cardiovascular diseases, and hypertension. The increasing prevalence of obesity, driven by sedentary lifestyles, poor dietary habits, genetic predisposition, underscores the urgent need for effective therapeutic strategies. Conventional pharmacological treatments, including appetite suppressants modulators, often fail provide sustainable weight loss due side effects, adherence, limited long-term efficacy. As result, natural bioactive compounds have gained attention their anti-obesity potential. However, clinical application hindered bioavailability, rapid metabolism, inefficient delivery. Lipid-based nano-carriers, liposomes, solid lipid nanoparticles, nanostructured carriers, offer promising solution enhancing solubility, stability, targeted delivery these compounds. These advanced systems improve retention, enable controlled release, enhance action on adipose tissue pathways. Additionally, functionalized stimulus-responsive nanocarriers present innovative approaches precision obesity treatment. Despite advancements, challenges remain in large-scale production, regulatory approval, safety. Overcoming barriers critical ensuring successful translation nano-formulated therapies. This review explores potential lipid-based nano-carriers optimizing efficacy highlights role advancing next-generation management
Язык: Английский
Процитировано
0
Frontiers in Veterinary Science, Год журнала: 2025, Номер 12
Опубликована: Май 16, 2025
Canine osteoarthritis (OA) stands as a prevalent and excruciating joint condition that represents promising application of stromal vascular fraction (SVF) treatment. In this review, we discuss the multi-factorial advantage SVF cited anti-inflammatory, regenerative, angiogenic, all which improve complex pathology OA. The heterogeneous cellular structure allows it to achieve improvement through both cell-based tissue restoration signaling functions benefit health. Veterinary practitioners need consider essential aspects for clinical practice including patient selection criteria together with specific dosage recommendations along additional therapies like platelet-rich plasma. Existing data shows reduces pain while helping restore face challenges when standardizing protocols evaluating long-term safety aspects. Future research initiatives aim translate advanced technologies bioactive scaffolds, gene editing, artificial intelligence show promise enhancing therapeutic results. This review integrates existing information about enlightening veterinarians gap areas assist them make informed decisions implementing in practice. huge step forward veterinary regenerative medicine because enables better management OA well other orthopedic applications.
Язык: Английский
Процитировано
0
The Journal of Basic and Applied Zoology, Год журнала: 2025, Номер 86(1)
Опубликована: Май 31, 2025
Abstract Background Adipose-derived stromal vascular fraction (AdSVF) and adipose-derived mesenchymal stem cells (AdMSCs) are promising tools in regenerative medicine due to their accessibility therapeutic potential. This study focuses on the vitro characterization of rat-derived AdMSCs (r-AdMSCs) isolated from AdSVF (r-AdSVF) for mesodermal tissue regeneration. Results r-AdSVF was through enzymatic digestion subcutaneous ventral abdominal fat depots, yielding an average 5.39 ± 0.73 × 10 6 per gram adipose tissue. The differentiated into under optimized culture conditions, with P3 achieving 90% confluence within five days exhibiting fibroblast-like morphology. Comprehensive included morphological analysis, immunocytochemistry, gene expression studies, trilineage differentiation (osteogenic, chondrogenic, adipogenic), colony-forming unit assays. r-AdMSCs showed positive markers (CD73, CD90, CD105) negative hematopoietic (CD34, CD45), confirming cell identity. Colony-forming assays demonstrated robust osteogenic potential, supporting application bone Conclusions highlights properties while emphasizing need improved standardization advance use engineering. By establishing a foundational framework, this research contributes developing safe, effective, accessible therapies repair
Язык: Английский
Процитировано
0
Comprehensive physiology, Год журнала: 2025, Номер 15(3)
Опубликована: Июнь 1, 2025
ABSTRACT Adipose tissue serves not only as a storage organ but also plays an active role in maintaining the body's homeostasis endocrine component. Mainly made up of adipocytes that store energy triglyceride droplets, this contains fibroblasts, immune cells, neuronal and endothelial cells. Collectively, these non‐adipocyte cells are known stromal vascular fraction. Evidence suggests both quantity quality adipose crucial regulating physiology by influencing lipid metabolism secreting important signaling molecules called adipokines. This review aims to systematically explore complex effects on regulation with particular focus two well‐characterized adipokines—leptin adiponectin—whose receptors abundantly expressed vasculature. We further aim provide overview classical recent research emphasize significance interplay between biology.
Язык: Английский
Процитировано
0
Chinese Journal of Plastic and Reconstructive Surgery, Год журнала: 2024, Номер unknown
Опубликована: Сен. 1, 2024
Процитировано
0