G3BP1, a stress granule core protein, ameliorates metabolic dysfunction‐associated fatty liver disease by attenuating hepatocyte lipid deposition DOI

Xingjing Liu,

Huimei Yu,

Tongtong Hu

и другие.

Diabetes Obesity and Metabolism, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

Abstract Aim Abnormal lipid accumulation is an important cause of metabolic dysfunction‐associated fatty liver disease (MAFLD) progression and can induce several stress responses within cells. This study the first to explore role molecular mechanism granules (SGs) in MAFLD. Methods A gene knock‐down model G3BP1, a core SG molecule mice HepG2 cells, was constructed SGs MAFLD induced vivo by high‐fat diet or vitro palmitic acid (PA). included phenotyping; western blotting; qPCR; immunofluorescence, haematoxylin/eosin masson staining. The downstream molecules G3BP1 its specific were screened using RNA sequencing (RNA‐seq). Results TIA1 expression upregulated diet‐fed mouse tissues PA‐induced two showed significantly increased colocalisation. slightly livers obese but not lean mice. deficiency aggravated deposition insulin resistance mice, this phenotype confirmed hepatocytes. RNA‐seq demonstrated that slowed down inhibiting APOC3, possibly through mechanistic suppression APOC3 entry into nucleus. Conclusion reveals for time protective Specifically, knocking acid‐induced may involve nuclear APOC3. These findings provide new therapeutic direction

Язык: Английский

Rethinking transcription factor dynamics and transcription regulation in eukaryotes DOI
Reiner A. Veitia

Trends in Biochemical Sciences, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Role of Assemblysomes in Cellular Stress Responses DOI Creative Commons

Bence György Gombás,

Orsolya Németh‐Szatmári,

Bence Nagy‐Mikó

и другие.

Wiley Interdisciplinary Reviews - RNA, Год журнала: 2025, Номер 16(2)

Опубликована: Март 1, 2025

ABSTRACT Assemblysomes are recently discovered intracellular RNA–protein complexes that play important roles in cellular stress response, regulation of gene expression, and also co‐translational protein assembly. In this review, a wide spectrum overview assemblysomes is provided, including their discovery, mechanism action, characteristics, potential applications several fields. distinct liquid–liquid phase‐separated condensates; they have certain unique properties differentiating them from other granules. They composed ribosome‐nascent chain resistant to cycloheximide EDTA. The discovery observation condensates, like assemblysomes, further expanded our knowledge response mechanisms, particularly DNA repair processes defense against proteotoxicity. Ribosome profiling experiments next‐generation sequencing cDNA libraries extracted EDTA‐resistant pellets—of ultracentrifuged cell lysates—have shed light on the composition dynamics revealing role as repositories for pre‐made stress‐responsive complexes. This review gives an exploration assemblysomes' clinical multiple aspects, usefulness diagnostic biomarkers chemotherapy resistance implications cancer therapy. addition, overview, we raise some theoretical ideas industrial agricultural connected these membraneless organelles. However, see challenges. On one hand, need understand complexity functions regulations; it essential bridge gap between fundamental research practical applications. Overall, assemblysome can be perceived promising upcomer improvement biomedical settings well those aspects.

Язык: Английский

Процитировано

0
G3BP1, a stress granule core protein, ameliorates metabolic dysfunction‐associated fatty liver disease by attenuating hepatocyte lipid deposition DOI

Xingjing Liu,

Huimei Yu,

Tongtong Hu

и другие.

Diabetes Obesity and Metabolism, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

Abstract Aim Abnormal lipid accumulation is an important cause of metabolic dysfunction‐associated fatty liver disease (MAFLD) progression and can induce several stress responses within cells. This study the first to explore role molecular mechanism granules (SGs) in MAFLD. Methods A gene knock‐down model G3BP1, a core SG molecule mice HepG2 cells, was constructed SGs MAFLD induced vivo by high‐fat diet or vitro palmitic acid (PA). included phenotyping; western blotting; qPCR; immunofluorescence, haematoxylin/eosin masson staining. The downstream molecules G3BP1 its specific were screened using RNA sequencing (RNA‐seq). Results TIA1 expression upregulated diet‐fed mouse tissues PA‐induced two showed significantly increased colocalisation. slightly livers obese but not lean mice. deficiency aggravated deposition insulin resistance mice, this phenotype confirmed hepatocytes. RNA‐seq demonstrated that slowed down inhibiting APOC3, possibly through mechanistic suppression APOC3 entry into nucleus. Conclusion reveals for time protective Specifically, knocking acid‐induced may involve nuclear APOC3. These findings provide new therapeutic direction

Язык: Английский

Процитировано

0