Genomic and functional adaptations in guanylate-binding protein 5 (GBP5) highlight specificities of bat antiviral innate immunity
Amandine Le Corf,
Sarah Maesen,
Clara Loyer
и другие.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 15, 2025
Bats
are
asymptomatic
reservoirs
of
several
zoonotic
viruses.
This
may
result
from
long-term
coevolution
between
viruses
and
bats,
that
have
led
to
host
adaptations
contributing
an
effective
balance
strong
antiviral
responses
with
innate
immune
tolerance.
To
better
understand
these
virus-host
interactions,
we
combined
comparative
transcriptomics,
phylogenomics
functional
assays
characterize
the
evolution
bat
factors.
First,
stimulated
type
I
interferon
pathway
in
Myotis
yumanensis
primary
cells
identified
guanylate-binding
protein
5
(GBP5)
as
most
differentially
expressed
interferon-stimulated
gene
(ISG).
Phylogenomic
analyses
showed
GBP5
has
been
under
episodic
positive
selection,
numerous
rapidly
evolving
sites
species-specific
duplications,
suggesting
past
evolutionary
arms
races.
Functional
tests
on
orthologs
ten
species
covering
>60
million
years
Chiroptera
revealed
species-
virus-specific
restrictions
against
RNA
(retrovirus
HIV,
rhabdoviruses
European
lyssavirus
VSV),
which
typical
signatures
viral
epidemics.
Interestingly,
also
observed
a
lineage-specific
loss
prenylation
motif
common
ancestor
Pipistrellus
Eptesicus
associated
different
subcellular
localization
functions.
Resurrection
ancestral
fuscus
rescued
its
localization,
but
not
complete
activities,
additional
determinants
necessary
for
restriction.
Altogether,
our
results
highlight
contribute
specific
immunity
provide
insights
into
effector
GBP5.
is
upon
stimulation
cells.
Bat
evolved
genomic
genetic
diversification,
including
early
stop
codon
leading
truncation
motif.GBP5
diversification
bats
impacts
their
functions.Bat
GBP5s
exhibit
virus-specificity
ability
inhibit
infectivity
particles,
bearing
glycoproteins
retroviral
vesicular
stomatitis
virus
lyssavirus-1.Resurrection
rescues
full
activity.
Язык: Английский
Expanding the bat toolbox: Carollia perspicillata bat cell lines and reagents enable the characterization of viral susceptibility and innate immune responses
PLoS Biology,
Год журнала:
2025,
Номер
23(4), С. e3003098 - e3003098
Опубликована: Апрель 15, 2025
Multiple
viruses
that
are
highly
pathogenic
in
humans
known
to
have
evolved
bats.
How
bats
tolerate
infection
with
these
viruses,
however,
is
poorly
understood.
As
engage
a
wide
range
of
interactions
their
hosts,
it
essential
study
bat
system
resembles
natural
environment
like
bat-derived
vitro
cellular
models.
However,
stable
and
accessible
cell
lines
not
widely
available
for
the
broader
scientific
community.
Here,
we
generated
reagents
Seba’s
short-tailed
(
Carollia
perspicillata
),
tested
multiple
methods
immortalization,
characterized
susceptibility
virus
response
immune
stimulation.
Using
pseudotyped
library
authentic
infections,
show
C.
derived
from
diverse
array
tissues
susceptible
bearing
glycoprotein
numerous
orthohantaviruses,
including
Andes
Hantaan
also
live
hantavirus
infection.
Furthermore,
stimulation
synthetic
double-stranded
RNA
prior
vesicular
stomatitis
Middle
Eastern
respiratory
syndrome
coronavirus
induced
protective
antiviral
response,
demonstrating
suitability
our
response.
Taken
together,
approaches
outlined
here
will
inform
future
efforts
develop
tools
virology
non-model
organisms
enable
studies
on
virus–host
Язык: Английский
<i>Myotis</i> bat STING attenuates aging-related inflammation in female mice
动物学研究,
Год журнала:
2024,
Номер
45(5), С. 961 - 971
Опубликована: Янв. 1, 2024
Bats,
notable
as
the
only
flying
mammals,
serve
natural
reservoir
hosts
for
various
highly
pathogenic
viruses
in
humans
(e.g.,
SARS-CoV
and
Ebola
virus).
Furthermore,
bats
exhibit
an
unparalleled
longevity
among
mammals
relative
to
their
size,
particularly
Язык: Английский
Bats as instructive animal models for studying longevity and aging
Annals of the New York Academy of Sciences,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 4, 2024
Abstract
Bats
(order
Chiroptera)
are
emerging
as
instructive
animal
models
for
aging
studies.
Unlike
some
common
laboratory
species,
they
meet
a
central
criterion
studies:
live
long
time
in
the
wild
or
captivity,
20,
30,
and
even
>40
years.
Healthy
(i.e.,
healthspan)
bats
has
drawn
attention
to
their
potential
improve
lives
of
humans
due
bat
imperviousness
viral
infections,
apparent
low
rate
tumorigenesis,
unique
ability
repair
DNA.
At
same
time,
longevity
also
permits
accumulation
age‐associated
systemic
pathologies
that
can
be
examined
detail
manipulated,
especially
captive
animals.
Research
uncovered
additional
critical
advantages
bats.
In
multiple
ways,
better
analogs
than
rodents.
this
review,
we
highlight
eight
diverse
areas
research
with
relevance
aging:
genome
sequencing,
telomeres,
DNA
repair;
immunity
inflammation;
hearing;
menstruation
menopause;
skeletal
system
fragility;
neurobiology
neurodegeneration;
stem
cells;
senescence
mortality.
These
examples
demonstrate
broad
an
model
point
directions
particularly
important
human
Язык: Английский
Studying bats using a One Health lens: bridging the gap between bat virology and disease ecology
Journal of Virology,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 5, 2024
ABSTRACT
Accumulating
data
suggest
that
some
bat
species
host
emerging
viruses
are
highly
pathogenic
in
humans
and
agricultural
animals.
Laboratory-based
studies
have
highlighted
important
adaptations
immune
systems
allow
them
to
better
tolerate
viral
infections
compared
humans.
Simultaneously,
ecological
discovered
critical
extrinsic
factors,
such
as
nutritional
stress,
correlate
with
virus
shedding
wild-caught
bats.
Despite
progress
independently
understanding
the
role
of
bats
reservoirs
viruses,
there
remains
a
significant
gap
molecular
factors
drive
spillover
from
Driven
by
collective
goal
bridging
between
fields
virology,
immunology,
disease
ecology,
we
hosted
satellite
symposium
at
2024
American
Society
for
Virology
meeting.
Bringing
together
virologists,
immunologists,
ecologists,
discussed
intrinsic
receptor
engagement,
adaptive
immunity,
ecology
influence
hosts.
This
article
summarizes
topics
during
emphasizes
need
interdisciplinary
collaborations
resource
sharing.
Язык: Английский