IgE recognition of the house dust mite allergen Der p 37 is associated with asthma

Journal of Allergy and Clinical Immunology, Год журнала: 2021, Номер 149(3), С. 1031 - 1043

Опубликована: Авг. 20, 2021

BackgroundHouse dust mite (HDM) allergens are major elicitors of allergic reactions worldwide.ObjectiveIdentification, characterization, and evaluation diagnostic utility a new important HDM allergen was performed.MethodsA cDNA coding for Dermatophagoides pteronyssinus (Dp) allergen, Der p 37, isolated from Dp expression library with patients' IgE antibodies. Recombinant 37 (rDer 37) expressed in Escherichia coli purified, then characterized by mass spectrometry, circular dichroism, reactivity ImmunoCAP ISAC technology sera 111 clinically defined HDM-allergic patients. The allergenic activity rDer studied basophil activation CD4+ T-cell responses carboxyfluorescein diacetate succinimidyl ester dilution assays. Specific antibodies raised against were used the ultrastructural localization mites immunogold transmission electron microscopy.ResultsDer 26 kDa homology to chitin-binding proteins, is immunologically distinct 15, 18, 23. It located peritrophic membrane fecal pellets. reacted third patients induced specific basophil- activation. IgE–positive had significantly higher levels allergens, more risk (odds ratio = 3.1) asthma compared 37–negative patients.ConclusionsDer recognized patients, may serve as surrogate marker severe sensitization asthma. House worldwide. Identification, performed. A microscopy.

Язык: Английский

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Molecular reactivity profiling upon immunotherapy with a 300 IR sublingual house dust mite tablet reveals marked humoral changes towards major allergens

Allergy, Год журнала: 2022, Номер 77(10), С. 3084 - 3095

Опубликована: Апрель 27, 2022

Molecular antibody reactivity profiles have not yet been studied in depth patients treated by sublingual house dust mite (HDM) tablet immunotherapy. Humoral immune responses to a large panel of HDM allergens were using allergen microarray technology subset clinically defined high and low responder from double-blind placebo-controlled allergen-specific immunotherapy (AIT) trial 300 IR tablets.Serum levels IgE, IgG IgG4 13 Dermatophagoides pteronyssinus molecules measured at baseline after 1-year AIT, microarrays 100 subjects exhibiting or clinical benefit.Der p 1, Der 2 23 the most frequently recognized study population. Patients with HDM-related asthma had significantly higher IgE 1 23. No significant difference distribution sensitization pattern was observed between responders. An increase serum occurred upon particular (p < 0.0001).We confirm for our population that 1- 23-specific are associated asthma. predicitive AIT outcomes, tablets as efficacious pauci- multi-sensitized subjects. Our is first demonstrate induction specific AIT.

Язык: Английский

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Clinical and immunological differences between asymptomatic HDM‐sensitized and HDM‐allergic rhinitis patients

Clinical & Experimental Allergy, Год журнала: 2019, Номер 49(6), С. 808 - 818

Опубликована: Фев. 8, 2019

Confirmation of the clinical relevance sensitisation is important for diagnosis allergic rhinitis.To investigate usefulness an in vitro basophil activation test and component-resolved distinguishing between symptomatic rhinitis patients asymptomatic sensitization to house dust mites (HDMs).Thirty-six subjects with a positive skin prick (SPT) HDM were divided into (n = 17) 19) group on basis their history nasal provocation test. A CD63 response stimulation Dermatophagoides pteronyssinus whole allergen extract IgE reactivity profiles Der p 1, 2, 4, 5, 7, 10, 11, 14, 15, 18, 21, 23 evaluated. Serum IgG specific D total measured.There no statistically significant differences levels (IgE higher P 0.055) IgE. Symptomatic showed lower threshold (3.33 ng/mL vs 33.3 ng/mL), area under curve (AUC) (171 127) (P 0.017), control anti-FcεRI (97% 79%) < 0.001), recognition more allergens (4 2) frequent rDer 7 0.016) compared 0.018). There was correlation (r 0.63; 0.001) number recognized AUC activation.In studied, extract, distinguish from better than SPT or extract-specific Information regarding prescription allergen-specific immunotherapy.

Язык: Английский

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IgE‐reactivity profiles to allergen molecules in Russian children with and without symptoms of allergy revealed by micro‐array analysis

Pediatric Allergy and Immunology, Год журнала: 2020, Номер 32(2), С. 251 - 263

Опубликована: Сен. 1, 2020

Abstract Background The analysis of longitudinal birth cohorts with micro‐arrayed allergen molecules has provided interesting information about the evolution IgE sensitization in children. However, so far no cross‐sectional study been performed comparing profiles children and without symptoms allergy. Furthermore, data are available regarding molecular from Russia. Methods We recruited two groups age‐ gender‐matched children, one (Group 1: n = 103; 12.24 ± 2.23 years; male/female: 58/45) a second 2: 97; 12.78 53/44), allergy according to international ISAAC questionnaire. Children were further studied (rhinitis, asthma, atopic dermatitis) guidelines, skin prick testing panel aeroallergen extracts was before sera analyzed an investigator‐blinded manner for specific more than 160 using ImmunoCAP ISAC technology. Results or >0.3 ISU at least found 100% symptomatic 36% asymptomatic Symptomatic showed comparable profile; however, frequencies levels individual higher Aeroallergen dominated by major birch pollen allergen, Bet v 1, cat Fel d 1. Food due cross‐sensitization PR10 food allergens whereas genuine peanut absent. Conclusion This is first analyzing It detects similar identifies 1 as predominant respiratory proteins absence Moscow region (Russia).

Язык: Английский

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Allergic March in Children: The Significance of Precision Allergy Molecular Diagnosis (PAMD@) in Predicting Atopy Development and Planning Allergen-Specific Immunotherapy

Nutrients, Год журнала: 2023, Номер 15(4), С. 978 - 978

Опубликована: Фев. 15, 2023

The allergic march is a progression of naturally occurring symptoms whose nature changes with age. classic typically begins in infancy and manifests the form atopic dermatitis food allergy. As immune tolerance develops over time, these conditions may resolve by age 3–5 years; however, they evolve into rhinitis bronchial asthma. Traditional diagnostic assessments, such as skin prick testing or serum allergen-specific immunoglobulin E (sIgE) level testing, are conducted to introduce effective treatment. Recent years saw emergence precision allergy molecular diagnosis (PAMD@), which assesses sIgE against allergenic molecules. This new technology helps more accurately evaluate patient’s profile, create precise dietary specifications personalize immunotherapy. review presents possible predictions regarding means controlling it based on PAMD@ results.

Язык: Английский

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