Prospective piperacillin lymphocyte transformation testing in patients with cystic fibrosis receiving regular and desensitization courses of piperacillin-tazobactam

The Journal of Allergy and Clinical Immunology In Practice, Год журнала: 2024, Номер 13(3), С. 594 - 609.e8

Опубликована: Дек. 12, 2024

Piperacillin-tazobactam is used in patients with cystic fibrosis to treat recurrent respiratory infections. Exposure associated a high frequency of nonimmediate hypersensitivity. To assess the applicability lymphocyte transformation test (LTT) for diagnosis piperacillin hypersensitivity and influence desensitization on piperacillin-specific T-cell responses. Study arm 1 was an analysis LTT responses from 58 naive/baseline tolerant samples collected over 3-year interventional phase. In study 2, 17 hypersensitive were recruited LTTs conducted before after desensitization. Clinical reactions both arms monitored 8-year observational period. 1, received 611 piperacillin-tazobactam courses (range, 2-40; mean ± SD, 10.5 8.1) during phase; 11 developed The who remained 236 period, 9 whom Ten phase had positive whereas one negative. We recorded 136 negative 39 patients, eight four LTT-positive 2 underwent desensitization, seven tolerating drug. strength decreased results minimum 14 days. During follow-up, tolerated 62 through sensitive marker drug sensitization that could be inform future patient management. Desensitization attenuation response.

Язык: Английский

Use of Antibody Structural Information in Disease Prediction Models Reveals Antigen Specific B Cell Receptor Sequences in Bulk Repertoire Data

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 15, 2024

Abstract Convergent antibodies are highly similar elicited in multiple individuals response to the same antigen. provide insight into shared immunological responses and show great promise as diagnostic biomarkers. They have typically been identified using methods that consider amino acid sequence of third complementarity-determining region (CDR3) immunoglobulin heavy chain (IgH). In this study, we extend definition convergent use structural information about three IgH CDR regions (CDR1-3). We benchmark performance both definitions convergence by their ability predict disease status from bulk sequencing data for two different diseases (HIV infection food sensitization). predicted outperforms prior approaches prediction sensitization performs on par HIV status. Additionally, structurally antibody groups driving known binders. Thus, allows identification antigen specific data.

Язык: Английский