Ultrasound-Assisted Deep Eutectic Solvent Extraction of Antioxidant and Anti-Colorectal Cancer Proteins from Spirulina Biomass: Process Intensification, Characterization, and Bioactivity Evaluation

Antioxidants, Год журнала: 2025, Номер 14(3), С. 365 - 365

Опубликована: Март 19, 2025

Spirulina, a cyanobacterial biomass, is renowned for its high protein content and bioactive compounds, making it promising candidate health-promoting applications. This study explores the ultrasound-assisted deep eutectic solvent (DES) extraction technique isolating antioxidants anticancer proteins from Spirulina aiming to enhance efficiency preserve bioactivity. The process was optimized using response surface methodology (RSM), varying parameters such as biomass concentration, sonication amplitude, duration. conditions—5% 40% 22-minute extraction—achieved yield of 80.62%, with concentration 442.88 mg/g extract an essential amino acid 39.91%. extracted exhibited remarkable bioactivity, including strong antioxidant properties, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity 0.25 mg GAE/g, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) 0.58 TE/g, ferric reducing power (PFRAP) 9.63 gallic equivalent (GAE)/g. Additionally, displayed selective cytotoxicity against colorectal cancer cell lines, half-maximal inhibitory (IC50) values 10.25 mg/mL Caco-2 15.40 HT-29 cells, while maintaining low toxicity towards normal Vero cells. Apoptosis rates 70.43% in 51.33% cells further confirm potential extract. functional properties protein, foaming capacity (100%), emulsifying (94.05%), digestibility (85.77%), underscore diverse applications food, pharmaceutical, nutraceutical industries. eco-friendly efficient approach aligns sustainable development goals offers viable strategy harnessing Spirulina’s potential.

Язык: Английский

Microwave-assisted extraction of proteins from Pseudochlorella pringsheimii: Optimization and application in fortified high-protein buns

Food Chemistry, Год журнала: 2025, Номер unknown, С. 144061 - 144061

Опубликована: Март 1, 2025

Язык: Английский