Опубликована: Ноя. 22, 2024
Язык: Английский
Current Opinion in Allergy and Clinical Immunology, Год журнала: 2024, Номер 24(2), С. 79 - 87
Опубликована: Фев. 7, 2024
Purpose of review To the current state knowledge on relationship between allergic sensitization and asthma; to lay out a roadmap for development IgE biomarkers that differentiate, in individual sensitized patients, whether their is important or future asthma symptoms, has little no relevance disease. Recent findings The evidence suggests some subtypes are not associated with whilst others pathologic. Interaction patterns antibodies allergenic molecules component-resolved diagnostics (CRD) multiplex arrays might be hallmarks by which different relevant can distinguished. These amongst individuals at all ages, clinical presentations (no disease, as single multimorbidity); preschool children without lower airway risk subsequent development; patients asthma, differing levels severity. Summary use machine learning-based methodologies complex CRD data help us design better diagnostic tools practising physicians differentiate benign clinically sensitization.
Язык: Английский
Процитировано
6
The Journal of Allergy and Clinical Immunology In Practice, Год журнала: 2024, Номер unknown
Опубликована: Авг. 1, 2024
Accurate identification of allergy-eliciting stinging insect(s) is essential to ensuring effective management Hymenoptera venom-allergic individuals with venom-specific immunotherapy. Diagnostic testing using whole-venom extracts skin tests and serologic-based analyses remains the first level discrimination for honeybee versus vespid venom sensitization in patients a positive clinical history. As second-level evaluation, serologic molecular allergens can further discriminate genuine (honeybee venom: Api m 1, 3, 4, 10 vs yellow jacket venom/Polistes dominula Ves v 1/Pol d 1 5/Pol 5) from interspecies cross-reactivity (hyaluronidases [Api 2, Pol 2] dipeptidyl peptidases IV 5, 3]). Clinical laboratories use number singleplex, oligoplex, multiplex immunoassays that employ both extracted (highlighted earlier) confirmation allergic sensitization. Established quantitative singleplex autoanalyzers have general governmental regulatory clearance worldwide patient maximally achievable analytical sensitivity (0.1 kUA/L) confirmed reproducibility (interassay coefficient variation <10%). Emerging oligoplex (fixed-panel) assays conserve on serum are more cost-effective, but they need some countries prone higher rates detecting asymptomatic Ultimately, patient's history, combined proof sensitization, final arbiter diagnosis allergy.
Язык: Английский
Процитировано
4
Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 229 - 249
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Computers in health care, Год журнала: 2025, Номер unknown, С. 195 - 210
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Clinical Chemistry and Laboratory Medicine (CCLM), Год журнала: 2024, Номер 62(12), С. 2339 - 2355
Опубликована: Май 30, 2024
Abstract Identification of the molecular culprits allergic reactions leveraged allergology applications in clinical laboratory medicine. Molecular shifted focus from complex, heterogeneous allergenic extracts, e.g. pollen, food, or insect venom, towards genetically and immunologically defined proteins available for vitro diagnosis. is a precision medicine approach diagnosis, stratification, therapeutic management, follow-up prognostic evaluation patients within large range diseases. Exclusively nonredundant with any current tools allergy investigation. As an example major application, discrimination genuine sensitization allergen cross-reactivity at level allows proper targeting culprit thus dramatically improves patient management. This review aims introducing specialists to allergology, biochemical genetic bases, through immunological concepts, daily use diagnosis management
Язык: Английский
Процитировано
2
Journal of Asthma, Год журнала: 2024, Номер 61(9), С. 1050 - 1057
Опубликована: Фев. 28, 2024
Screening asthma patients for atopy facilitates management. Since 2010, the core biomarker screening subjects atopic status has been qualitative Phadiatop. multi-aeroallergen screen. A more quantitative macroarray, Allergy Explorer (ALEX
Язык: Английский
Процитировано
1
Frontiers in Allergy, Год журнала: 2024, Номер 5
Опубликована: Июль 15, 2024
In the United States, 19 allergen extracts of different specificities are standardized, which means that their potencies determined in comparison to a US reference standard. For cat extracts, potency is by measuring Fel d 1 content expressed units, and with unitage correlates skin test reactions (bioequivalent allergy units or BAU). Currently, measured radial immunodiffusion (RID) assay uses polyclonal sheep antisera detect allergenic protein producing white precipitin line agar gel. However, RID considered cumbersome, sera may qualitatively vary among animals recognize epitopes irrelevant human allergic disease. this report, we describe quantitative two-site immunoenzymetric (IEMA) for immobilized capture soluble biotin-labeled detection 1-specific IgE monoclonal antibodies (mAb) have been class-switched IgG4. Together, they sandwich molecules from extracts. Using purified natural as calibrator, historically reported ∼4 micrograms 1/Fel unit assignment was directly mAb-based IEMA at 3.12 ± 0.24 per unit. This appears be equivalent measurement biological commercial hair pelt marketed States.
Язык: Английский
Процитировано
1
The Journal of Allergy and Clinical Immunology In Practice, Год журнала: 2023, Номер 11(10), С. 3008 - 3009
Опубликована: Авг. 22, 2023
Язык: Английский
Процитировано
2
Опубликована: Ноя. 22, 2024
Язык: Английский
Процитировано
0