Environmental endocrine disrupting chemical-DEHP exposure-provoked biotoxicity about microbiota-gut-mammary axis in lactating mice via multi-omics technologies

Environment International, Год журнала: 2024, Номер 193, С. 109130 - 109130

Опубликована: Ноя. 1, 2024

Plastics, pervasive in humans and nature, often contain Di (2-ethylhexyl) phthalate (DEHP) that enhance plastic's elasticity. However, DEHP is an environmental endocrine disruptor, affecting organisms upon exposure. Understanding mammary gland development lactating females crucial for offspring nourishment dairy production. Employing multi-omics technology, this study aimed to uncover DEHP's impact on the microbial-gut-mammary axis. Forty mice were exposed varying doses 18 d. We performed 16S sequencing, metabolomics, tissue observation, gene expression profiling. Results revealed influence microbial diversity, with increased Lactobacillus abundance reduced Proteobacteria, alongside colonic inflammation. Elevated GMP adenosine 5'-monophosphate levels bloodstream noted, while ascorbic acid, glycitein, others decreased. MEHP, a metabolite, damaged tissues, inhibiting ERK1/2 phosphorylation, triggering apoptosis ferroptosis. These findings unveil potential therapeutic targets DEHP-induced chronic toxicity animals, aiding livestock health human well-being. This underscores importance of understanding adverse effects exposure mammalian systems.

Язык: Английский

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Beneficial effects of Saccharomyces cerevisiae fermentation postbiotic products on calf and cow health and plausible mechanisms of action

Frontiers in Animal Science, Год журнала: 2024, Номер 5

Опубликована: Ноя. 20, 2024

Supplementation of cattle diets with Saccharomyces cerevisiae fermentation products (SCFP) has been shown to improve health and performance calves cows in both the dairy feedlot. Numerous studies have SCFP supplementation is beneficial context production- infection-related stressors, promoting resilience, accelerated resolution inflammation or oxidative stress, enabling cow calf maintain homeostasis. SCFPs, derived from yeast fermentation, encompass a rich array bioactive compounds, including vitamins, minerals, amino acids, metabolites, which likely influence host through distinct overlapping processes. Understanding mechanisms by SCFPs exert their effects crucial for optimizing utilization production systems. In this review, we focused not only on but also microbiota, epithelial barrier integrity, immune system, providing mechanistic insights. Previous suggested that impact metabolism, modulate rumen hindgut microbial populations, antioxidant immunomodulatory effects, stimulate expression genes involved maintaining tissue integrity. However, there are still gaps understanding certain pathways, particularly those involving nervous as well paradoxical enhancing responses while simultaneously mitigating excessive inflammation. This review summarizes several recent reports describing benefits considers available evidence mode action.

Язык: Английский

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Exploring the relationship between gut microbiota and breast diseases using Mendelian randomization analysis

Frontiers in Medicine, Год журнала: 2024, Номер 11

Опубликована: Ноя. 26, 2024

Growing evidence suggests a relationship between gut microbiota composition and breast diseases, although the precise nature of this association remains uncertain. To investigate causal we utilized two-way Mendelian randomization (MR) analysis. Four common diseases were included as outcomes: cancer, cysts, inflammatory disorders breast, infections associated with childbirth, along their subtypes. Genetic data on extracted from genome-wide studies (GWAS). The primary approach used to these genetic factors was inverse-variance-weighted (IVW) method random-effects types. Sensitivity analyses, such Cochran's Q test, MR-Egger intercept leave-one-out analysis, conducted ensure stability reliability MR findings. We discovered plausible links 20 microbial categories significance level p < 0.05. Notably, Family.Rikenellaceae (p: 0.0013) maintained significant inverse overall cancer (BC), after Bonferroni correction. In reverse interactions observed Genus.Adlercreutzia estrogen receptor-positive cancer. addition, Genus.Sellimonas, Family.Rikenellaceae, Genus.Paraprevotella ER+ whereas Genus.Dorea linked both receptor-negative Family.Prevotellaceae only category correlated disorders. Moreover, Genus Eubacteriumruminantiumgroup, Genus.Lactococcus, Family.Alcaligenaceae while Genus.Anaerofilum, Genus.Butyricimonas, Order.Coriobacteriales, Order.Pasteurellales, Order.Verrucomicrobiales showed associations childbirth. No heterogeneity or horizontal pleiotropy found. Our analysis confirmed diseases. Early stool tests may be viable for screening identify people at higher risk

Язык: Английский

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Succinate exacerbates mastitis in mice via gut microbiota-derived extracellular vesicles

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 16, 2023

Abstract Background Ecological imbalance of the gut microbiota may be an important endogenous trigger mastitis, but underlying mechanisms are unclear. Recent findings indicated that selected bacteria maternal gastrointestinal can reach mammary gland through entero-mammary pathway. In addition, metabolic disturbances one most prominent ways in which dysbiosis promote distal organ diseases. Results present study, we found Gastrointestinal-associated mastitis showed significant changes levels, among succinate was significantly increased. treatment exacerbates endotoxemia-induced mice. Meanwhile, induced intestinal ecological dysregulation. Further experiments demonstrated increased production extracellular vesicles containing microbial (mEVs) lipopolysaccharides, could diffuse across damaged barrier into glands. mEVs promotes inflammation mainly activation TLR4/NF-κB Conclusions Our suggest proliferatio enteric pathogens and production, suggesting a potential strategy for intervention based on regulation pathogen inhibition. The role interspecific communication has also been elucidated.

Язык: Английский

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