Irisin reshapes bone metabolic homeostasis to delay age-related osteoporosis by regulating the multipotent differentiation of BMSCs via Wnt pathway
Frontiers in Molecular Biosciences,
Год журнала:
2025,
Номер
11
Опубликована: Янв. 7, 2025
Bone
aging
is
linked
to
changes
in
the
lineage
differentiation
of
bone
marrow
stem
cells
(BMSCs),
which
show
a
heightened
tendency
differentiate
into
adipocytes
instead
osteoblasts.
The
therapeutic
potential
irisin
addressing
age-related
diseases
has
garnered
significant
attention.
More
significantly,
capacity
enhance
mass
recovery
and
sustain
overall
health.
Its
mechanism
action
preventing
osteoporosis
generated
considerable
interest
within
research
community.
Nonetheless,
targeting
effect
on
its
underlying
molecular
biological
mechanisms
remain
unclear.
specific
role
osteogenic-adipogenic
young
or
BMSCs
was
evaluated
by
multiple
staining
quantitative
real-time
PCR
(RT-qPCR)
analysis.
RNA-seq
protein
Western
blotting
excavated
validated
key
pathway
influences
fate
determination
BMSCs.
macroscopic
microscopic
tissue
mice
were
examined
using
Micro-computed
tomography
(Micro-CT)
morphological
staining.
It
noted
that
affected
multilineage
manner
dependent
dosage.
Simultaneously,
Wnt
signaling
might
be
crucial
through
sustains
bone-fat
balance
mitigates
decline
pluripotency.
In
vivo,
reduced
fat
deposition
effectively
alleviating
occurrence
loss.
Irisin
mediates
pathway,
thereby
influencing
addition,
it
essential
for
preserving
metabolic
equilibrium
microenvironment
significantly
contributes
findings
provide
new
evidence
use
iris
extract
treatment
osteoporosis.
Язык: Английский
TLR4 mediates glucolipotoxicity-induced mitochondrial dysfunction in osteoblasts by enhancing NLRP3-MAVS expression and interaction
International Immunopharmacology,
Год журнала:
2025,
Номер
153, С. 114438 - 114438
Опубликована: Март 17, 2025
Язык: Английский
Irisin mitigates osteoporotic-associated bone loss and gut dysbiosis in ovariectomized mice by modulating microbiota, metabolites, and intestinal barrier integrity
BMC Musculoskeletal Disorders,
Год журнала:
2025,
Номер
26(1)
Опубликована: Апрель 16, 2025
Язык: Английский
Epicatechin: Potential Use as Anti-Obese and Anti-Periodontal Nutrient
Current Oral Health Reports,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 17, 2024
Comprehensive Investigation of Homology and Heterogeneity Between Aurantii Fructus and Aurantii Fructus Immaturus Using Chemometrics Combined with Network Pharmacology
Journal of Medicinal Food,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 30, 2024
Aurantii
Fructus
(AF)
and
Immaturus
(AFI)
are
distinct
herbs
outlined
by
the
Chinese
Pharmacopoeia.
They
sourced
from
same
plant
but
harvested
at
different
times,
resulting
in
differences
efficacy.
It
is
important
to
avoid
mixing
them
clinically
distinguish
between
two.
Furthermore,
dissimilar
cultivation
conditions
may
cause
variability
quality
of
herbs,
so
it
vital
differentiate
drugs
origins.
In
this
study,
two
plants,
AF
AFI
provinces,
were
comparatively
analyzed
based
on
High
Performance
Liquid
Chromatography
(HPLC)
fingerprints
classified
using
chemometric
methods.
The
results
indicate
that
medicines
can
be
clearly
distinguished.
Also,
grown
locations
Ten
chemical
markers
screened,
their
variations
determined,
including
eriocitrin,
narirutin,
naringin,
meranzin
hydrate,
naringenin,
hesperidin,
nobiletin,
tangeretin,
neohesperidin,
poncirin.
Subsequent
network
pharmacology
correlated
screened
components
with
biological
organism.
material
basis
difference
efficacy
homologous
was
explored
perspective
changes
composition.
This
study
provides
a
reference
for
formulating
evaluation
standards
lays
foundation
efficacy-related
research
Язык: Английский
TAZ reverses the inhibitory effects of LPS on the osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway
BMC Oral Health,
Год журнала:
2024,
Номер
24(1)
Опубликована: Июнь 26, 2024
Abstract
Background
Human
periodontal
ligament
stem
cells
(hPDLSCs)
are
important
candidate
seed
for
tissue
engineering,
but
the
presence
of
lipopolysaccharide(LPS)
in
tissues
inhibits
self-renewal
and
osteogenic
differentiation
hPDLSCs.
Our
previous
studies
demonstrated
that
TAZ
is
a
positive
regulator
hPDLSCs,
whether
can
protect
hPDLSCs
from
LPS
still
unknown.
The
present
study
aimed
to
explore
regulatory
effect
on
an
LPS-induced
inflammatory
model,
preliminarily
reveal
molecular
mechanisms
related
NF-κB
signaling
pathway.
Methods
was
added
culture
medium
influence
hPDLSC
proliferation
analyzed
by
CCK-8
assays.
effects
were
detected
Alizarin
Red
staining,
ALP
Western
Blot
qRT-PCR
analysis
osteogenesis-related
genes.
with
overexpressed
or
knocked
down
via
lentivirus
analyzed.
immunofluorescence.
Results
inhibited
expression,
activated
Overexpressing
partly
reversed
negative
activation
pathway
LPS.
knockdown
enhanced
inhibitory
osteogenesis.
Conclusion
could
reverse
possibly
through
inhibiting
potential
target
improving
hPDLSC-based
regeneration
environments.
Язык: Английский
Exercise Promotes Hippocampal Neurogenesis in T2DM Mice via Irisin/TLR4/MyD88/NF-κB-Mediated Neuroinflammation Pathway
Biology,
Год журнала:
2024,
Номер
13(10), С. 809 - 809
Опубликована: Окт. 10, 2024
Neuroinflammation
is
a
major
feature
of
type
2
diabetic
mellitus
(T2DM),
adversely
affecting
hippocampal
neurogenesis.
However,
the
precise
mechanism
not
fully
understood,
and
therapeutic
approaches
are
currently
lacking.
Therefore,
we
determined
effects
exercise
on
neuroinflammation
neurogenesis
in
T2DM
mice,
with
specific
focus
understanding
role
irisin
related
cascade
pathways
modulating
beneficial
these
processes.
Ten-week
significantly
decreased
T2DM-induced
inflammation
levels
markedly
promoted
memory
function.
positive
were
reversed
by
10
weeks
treatment
cyclo
RGDyk,
an
inhibitor
receptor
signaling.
Additionally,
helped
reduce
M1
phenotype
polarization
microglia
mice;
this
effect
could
be
RGDyk
treatment.
Moreover,
increased
fibronectin
III
domain-containing
protein
5
(FNDC5)/irisin
while
decreasing
expression
Toll-like
4
(TLR4),
myeloid
differential
protein-88
(MyD88),
nuclear
factor
kappa-B
(NF-κB)
hippocampus
mice.
blocking
signaling
counteracted
down-regulation
TLR4/MyD88/NF-κB
mice
undergoing
intervention.
Conclusively,
appears
to
effective
reducing
enhancing
diabetes
The
involved
participation
irisin/TLR4/MyD88/NF-κB
pathway,
highlighting
potential
management
diabetic-induced
cognitive
decline.
Язык: Английский