Osteoporosis and chronic tendinopathy: a two-sample Bidirectional Mendelian randomization DOI Creative Commons
Hongfei Wu, Yu Cui, Yun Gao

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 25, 2024

Abstract BACKGROUND The incidence of osteoporosis rises with advancing age, and it has emerged as a significant global public health issue, often presenting clinically symptoms such pain, kyphosis, reduced height, fractures.Chronic tendinopathy is common orthopedic disease, which mainly characterized by delayed difficult to heal, causing great pain patients. Preliminary epidemiological studies have investigated the potential association between chronic tendinopathy; however, definitive causal relationship yet be established. With increasing life expectancy an accelerating aging population, burden expected rise significantly, important implications for morbidity mortality.METHODS Instrumental variables were selected from IEU GWAS database summary statistics. Five different bone mineral density (BMD) sites—heel, total body, femoral neck, lumbar spine, ultradistal forearm BMD—along body BMD across five age groups (0–15, 15–30, 30–45, 45–60, over 60 years) utilized phenotypes. Achilles tendinitis, Bicipital Calcific tendinitis shoulder, Gluteal Patellar selected, Peroneal represent Chronic Multiple analytical methods employed comprehensively assess osteoporosis. Sensitivity analyses, including Cochran's Q test, MR-Egger intercept 'leave-one-out' analysis, conducted verify robustness findings.RESULTS Mendelian Randomization analysis revealed sites shoulder; Additionally, MR demonstrated Heel BMD, Lumbar spine tendinitis. In examining age-related tendinopathy, relationships identified in 0–15 years, 45–60 years calcific shoulder.. all inverse no was observed. reliability these results confirmed through sensitivity analyses.CONCLUSION Osteoporosis may etiological factor observed particularly individuals 45 age. This suggests that patients underlying issue Therefore, routine screening recommended who present tendinopathy.

Язык: Английский

Pyroptosis: candidate key targets for mesenchymal stem cell-derived exosomes for the treatment of bone-related diseases DOI Creative Commons
Hong Li, Peng Zhang, Minghui Lin

и другие.

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 12, 2025

Bone-related diseases impact a large portion of the global population and, due to their high disability rates and limited treatment options, pose significant medical economic challenges. Mesenchymal stem cells (MSCs) can differentiate into multiple cell types offer strong regenerative potential, making them promising for treating various diseases. However, issues with immune response survival limit effectiveness transplantation. This has led increased interest in cell-free therapy, particularly use exosomes, which is most studied form this approach. Exosomes are extracellular vesicles that contain proteins, lipids, nucleic acids play key role communication material exchange. Pyroptosis, death involved innate immunity, also associated many Studies have shown MSC-derived exosomes therapeutic potential range conditions by regulating inflammation pyroptosis. study explored modulating pyroptosis improve bone-related

Язык: Английский

Процитировано

1
Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration DOI Creative Commons
Zhuoming Xu, Jun Ma,

Hanyin Hu

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Янв. 28, 2025

Introduction Osteoarthritis (OA) is a degenerative disease of the joints characterized by cartilage degradation and synovial inflammation. Due to complex pathogenesis OA, multifaceted therapies that modulate inflammatory immune microenvironmental disturbances while promoting regeneration are key control progression OA. Methods Herein, multifunctional nanoparticle (DIC/Mg-PDA NPs) was constructed successfully metal chelation effect between Mg 2+ catecholamine bond from dopamine, followed amidation with diclofenac (DIC), which then prepared into an injectable hydrogel microsphere (DIC/Mg-PDA@HM) immune-regulating cartilage-repairing abilities through microfluidic technology for treatment osteoarthritis. Results discussion The sustained release composite microspheres achieved regulation converting macrophages M1 M2 promoted differentiation BMSCs. Moreover, enhanced DIC polydopamine (PDA) effectively downregulated factors, finally OA therapy. In addition, in vivo MRI tissue section staining model proved significant efficacy on conclusion, these novel demonstrated promising prospect multidisciplinary repairing

Язык: Английский

Процитировано

0
The communication role of extracellular vesicles in the osteoarthritis microenvironment DOI Creative Commons
Pu Chen, Lingfeng Zeng, Ting Wang

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 17, 2025

Osteoarthritis (OA) is the most common degenerative joint disease worldwide, characterized by synovial inflammation, cartilage loss, and reactive hyperplasia of subchondral bone, affecting quality life hundreds millions people. However, molecular mechanisms underlying occurrence progression OA remain unclear, there no therapy can substantially interrupt or reverse destructive process OA. More insight into pathogenesis may result in innovative therapeutics. The microenvironment plays a pivotal role development OA, which encompasses chondrocytes, adipocytes, fibroblasts, endothelial cells, immune cells. Extracellular vesicles (EVs) have emerged as novel form intercellular communication, mediating transfer range bioactive molecules to create specific microenvironment. Recent studies reported that cargos EVs play crucial including noncoding RNAs (ncRNAs), proteins, lipids. This review systematically analyzes summarizes biological characteristics functionalities derived from diverse cellular sources, especially how mediate communication between different cells microenvironment, with view providing new insights

Язык: Английский

Процитировано

0
LncRNA EMBP1 sponges miR-454-3p to upregulate IRF1 and activate NLRP3-mediated chondrocyte pyroptosis to drive osteoarthritis progression DOI Creative Commons

Shang Ma,

Peng Yu,

Jinxin Ma

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 153, С. 114460 - 114460

Опубликована: Март 20, 2025

Osteoarthritis (OA) is the most common degenerative joint disease worldwide. Studies have confirmed that pyroptosis closely associated with OA onset and progression, particularly via classical pathway mediated by NLRP3 inflammasome. However, intrinsic regulatory mechanisms underlying in remain unclear. We conducted RNA sequencing (RNA-seq) analysis on clinical cartilage samples identified hub genes connecting pyroptosis. validated NLRP3-mediated activation, evaluated diagnostic potential of gene, explored its role using a papain-induced rabbit model IL-1β-induced chondrocytes. Subsequently, we constructed competitive endogenous (ceRNA) network based gene binding interactions function Additionally, interferon factor 1 (IRF1) serves as recognized upstream regulator novel cell death paradigm PANoptosis, which integrates apoptosis, necrosis, preliminarily molecular PANoptosis through sample vitro experiments. RNA-seq revealed IRF1, linking pyroptosis, upregulated NLRP3, consistent vivo results. Dual-luciferase assays, analysis, experiments embigin pseudogene (EMBP1)/miR-454-3p/IRF1 ceRNA network. Silencing EMBP1 increased miR-454-3p, inhibiting IRF1 vitro; however, miR-454-3p inhibitor rescue abolished beneficial effects si-EMBP1. Furthermore, characterized occurrence provided initial evidence suggesting for EMBP1/miR-454-3p/IRF1 axis this process. In OA, acts sponge negative effect exacerbating chondrocyte EMBP1/miR-454-3p/IRF1-mediated may be integrated into broader process, interacting apoptosis necrosis to influence progression.

Язык: Английский

Процитировано

0
Periprosthetic osteolysis: Mechanisms and potential treatment strategies DOI
Fang Yao,

Yongneng Bao,

Qian Meng

и другие.

Cellular Signalling, Год журнала: 2025, Номер unknown, С. 111758 - 111758

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Pyroptosis for osteoarthritis treatment: insights into cellular and molecular interactions inflammatory DOI Creative Commons
Minghui Lin, Cunxin Zhang, Haiming Li

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 1, 2025

Osteoarthritis (OA) is a widely prevalent chronic degenerative disease often associated with significant pain and disability. It characterized by the deterioration of cartilage extracellular matrix (ECM), synovial inflammation, subchondral bone remodeling. Recent studies have highlighted pyroptosis-a form programmed cell death triggered inflammasome-as key factor in sustaining inflammation. Central to this process are inflammatory cytokines interleukin-1β (IL-1β) interleukin-18 (IL-18), which play crucial roles mediating intra-articular pyroptosis through NOD-like receptor protein 3 (NLRP3) inflammasome. This paper investigates role pathway perpetuating diseases its linkage OA. Furthermore, it explores mechanisms pyroptosis, mediated nuclear κB (NF-κB), purinergic P2X ligand-gated ion channel 7 (P2X7R), adenosine monophosphate (AMP)-activated kinase (AMPK), hypoxia-inducible factor-1α (HIF-1α). Additionally, examines interactions among various cellular components context These insights indicate that targeting regulation presents promising therapeutic approach for prevention treatment OA, offering valuable theoretical perspectives effective management.

Язык: Английский

Процитировано

0
Osteoporosis and chronic tendinopathy: a two-sample Bidirectional Mendelian randomization DOI Creative Commons
Hongfei Wu, Yu Cui, Yun Gao

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 25, 2024

Abstract BACKGROUND The incidence of osteoporosis rises with advancing age, and it has emerged as a significant global public health issue, often presenting clinically symptoms such pain, kyphosis, reduced height, fractures.Chronic tendinopathy is common orthopedic disease, which mainly characterized by delayed difficult to heal, causing great pain patients. Preliminary epidemiological studies have investigated the potential association between chronic tendinopathy; however, definitive causal relationship yet be established. With increasing life expectancy an accelerating aging population, burden expected rise significantly, important implications for morbidity mortality.METHODS Instrumental variables were selected from IEU GWAS database summary statistics. Five different bone mineral density (BMD) sites—heel, total body, femoral neck, lumbar spine, ultradistal forearm BMD—along body BMD across five age groups (0–15, 15–30, 30–45, 45–60, over 60 years) utilized phenotypes. Achilles tendinitis, Bicipital Calcific tendinitis shoulder, Gluteal Patellar selected, Peroneal represent Chronic Multiple analytical methods employed comprehensively assess osteoporosis. Sensitivity analyses, including Cochran's Q test, MR-Egger intercept 'leave-one-out' analysis, conducted verify robustness findings.RESULTS Mendelian Randomization analysis revealed sites shoulder; Additionally, MR demonstrated Heel BMD, Lumbar spine tendinitis. In examining age-related tendinopathy, relationships identified in 0–15 years, 45–60 years calcific shoulder.. all inverse no was observed. reliability these results confirmed through sensitivity analyses.CONCLUSION Osteoporosis may etiological factor observed particularly individuals 45 age. This suggests that patients underlying issue Therefore, routine screening recommended who present tendinopathy.

Язык: Английский

Процитировано

0