Alterations in Gene Expression and Alternative Splicing Induced by Plasmid-Mediated Overexpression of GFP and P2RY12 Within the A549 Cell Line DOI Open Access
Qingqing Liu, Zhaoyu Liu, Y. Qian

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2973 - 2973

Опубликована: Март 25, 2025

Phenotypic modifications and their effects on cellular functions through the up-regulation of target gene expression have frequently been observed in genetic studies, but unique roles cell lines introduced plasmids influencing these not fully revealed. In this research, we developed two distinct derived from A549 line: one that stably overexpresses GFP another is a polyclonal stable line overexpressing both P2RY12. We then utilized transcriptome sequencing (RNA-seq) technology to screen out differentially expressed genes (DEGs) with differential transcript usage (gDTUs) after overexpression (GFP-OE) P2RY12 (P2RY12-OE). found that, compared A549, there were more than 1700 GFP-OE P2RY12-OE cells, while only 866 DEGs identified cells. Notably, differences relatively minor, over 400 exhibiting changes across all three groups. The functional analysis gDTUs showed they highly enriched pathways associated proliferation migration. summary, performed an extensive profile alternative splicing P2RY12-OE, enhancing our comprehension how function within cells processes control expression.

Язык: Английский

Alterations in Gene Expression and Alternative Splicing Induced by Plasmid-Mediated Overexpression of GFP and P2RY12 Within the A549 Cell Line DOI Open Access
Qingqing Liu, Zhaoyu Liu, Y. Qian

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2973 - 2973

Опубликована: Март 25, 2025

Phenotypic modifications and their effects on cellular functions through the up-regulation of target gene expression have frequently been observed in genetic studies, but unique roles cell lines introduced plasmids influencing these not fully revealed. In this research, we developed two distinct derived from A549 line: one that stably overexpresses GFP another is a polyclonal stable line overexpressing both P2RY12. We then utilized transcriptome sequencing (RNA-seq) technology to screen out differentially expressed genes (DEGs) with differential transcript usage (gDTUs) after overexpression (GFP-OE) P2RY12 (P2RY12-OE). found that, compared A549, there were more than 1700 GFP-OE P2RY12-OE cells, while only 866 DEGs identified cells. Notably, differences relatively minor, over 400 exhibiting changes across all three groups. The functional analysis gDTUs showed they highly enriched pathways associated proliferation migration. summary, performed an extensive profile alternative splicing P2RY12-OE, enhancing our comprehension how function within cells processes control expression.

Язык: Английский

Процитировано

0