Macrophages and cardiac lesion in zebrafish: what can single-cell RNA sequencing reveal? DOI Creative Commons
Rebeca Bosso dos Santos Luz, André Guilherme Portela de Paula,

Andressa Pacheco Czaikovski

и другие.

Frontiers in Cardiovascular Medicine, Год журнала: 2025, Номер 12

Опубликована: Апрель 11, 2025

Unlike mammals, zebrafish can regenerate their heart after cardiac insult. There are several ways to perform injury in zebrafish, but cryoinjury most closely resembles human myocardial infarction (MI). Studies demonstrated that macrophages essential cells from the beginning later stages of throughout regenerative process zebrafish. These have phenotypic plasticity; hence, overly sensitive techniques, such as single-cell RNA sequencing (scRNAseq), for uncovering phenotype needed regeneration, inflammatory profile initiation scar resolution. This technique enables individual cells, thus generating clusters with similar gene expression and allowing study a particular cell population. Therefore, this review, we focused on discussing data obtained by scRNAseq context injury. We found 1 7 days post-injury (dpi), present reparative functions either or ventricular resection. At 14 dpi, there were differences between models, especially cytokines, studies time points understand enrolls collagen resorption dynamic.

Язык: Английский

Macrophages and cardiac lesion in zebrafish: what can single-cell RNA sequencing reveal? DOI Creative Commons
Rebeca Bosso dos Santos Luz, André Guilherme Portela de Paula,

Andressa Pacheco Czaikovski

и другие.

Frontiers in Cardiovascular Medicine, Год журнала: 2025, Номер 12

Опубликована: Апрель 11, 2025

Unlike mammals, zebrafish can regenerate their heart after cardiac insult. There are several ways to perform injury in zebrafish, but cryoinjury most closely resembles human myocardial infarction (MI). Studies demonstrated that macrophages essential cells from the beginning later stages of throughout regenerative process zebrafish. These have phenotypic plasticity; hence, overly sensitive techniques, such as single-cell RNA sequencing (scRNAseq), for uncovering phenotype needed regeneration, inflammatory profile initiation scar resolution. This technique enables individual cells, thus generating clusters with similar gene expression and allowing study a particular cell population. Therefore, this review, we focused on discussing data obtained by scRNAseq context injury. We found 1 7 days post-injury (dpi), present reparative functions either or ventricular resection. At 14 dpi, there were differences between models, especially cytokines, studies time points understand enrolls collagen resorption dynamic.

Язык: Английский

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