Plasma Exosomes Derived from Patients with Primary Immune Thrombocytopenia Attenuate TBX21+ Regulatory T Cell-Mediated Immune Suppression via MiR-363-3p DOI Creative Commons

Yuan‐Lan Huang,

Peng Liu, Ying Xu

и другие.

Inflammation, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Primary Immune Thrombocytopenia (ITP) is characterized by reduced immunosuppressive function of regulatory T cells (Tregs), contributing to immune imbalance and decreased platelet counts. However, the mechanisms behind this efficacy Tregs remain unclear. Our study used a variety methods, including Treg assays, cytokine analysis, single-cell sequencing, explore these mechanisms. We found that exosomes from ITP patients inhibited TBX21 expression in Tregs, impaired their ability suppress Th1 cells. At level, with high were identified, activity regulon was be enhanced early-stage subpopulations. also discovered ARID3A interacted SPI1 gene regions, indicating relationship between ARID3A, SPI1, TBX21. Additionally, patients' plasma contained elevated levels miR-363-3p, which negatively correlated count. These transferred miR-363-3p downregulating expression, thereby weakening Tregs' conventional CD4 + In conclusion, through ARID3A/SPI1/TBX21 axis diminishing regulate dysfunction observed ITP.

Язык: Английский

Plasma Exosomes Derived from Patients with Primary Immune Thrombocytopenia Attenuate TBX21+ Regulatory T Cell-Mediated Immune Suppression via MiR-363-3p DOI Creative Commons

Yuan‐Lan Huang,

Peng Liu, Ying Xu

и другие.

Inflammation, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Primary Immune Thrombocytopenia (ITP) is characterized by reduced immunosuppressive function of regulatory T cells (Tregs), contributing to immune imbalance and decreased platelet counts. However, the mechanisms behind this efficacy Tregs remain unclear. Our study used a variety methods, including Treg assays, cytokine analysis, single-cell sequencing, explore these mechanisms. We found that exosomes from ITP patients inhibited TBX21 expression in Tregs, impaired their ability suppress Th1 cells. At level, with high were identified, activity regulon was be enhanced early-stage subpopulations. also discovered ARID3A interacted SPI1 gene regions, indicating relationship between ARID3A, SPI1, TBX21. Additionally, patients' plasma contained elevated levels miR-363-3p, which negatively correlated count. These transferred miR-363-3p downregulating expression, thereby weakening Tregs' conventional CD4 + In conclusion, through ARID3A/SPI1/TBX21 axis diminishing regulate dysfunction observed ITP.

Язык: Английский

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