ATP synthase subunit ATP5B interacts with TGEV Nsp2 and acts as a negative regulator of TGEV replication DOI Creative Commons
Yanan Wang,

Aoying Sun,

Yaru Guo

и другие.

Virulence, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 6, 2024

Coronavirus nonstructural protein 2 (Nsp2) is regarded as a virulence determinant and plays critical role in virus replication, innate immunity. Screening identifying host cell proteins that interact with viral an effective way to reveal the functions of proteins. In this study, interacted transmissible gastroenteritis (TGEV) Nsp2 were identified using immunoprecipitation combined LC-MS/MS. 77 putative interaction protein-protein (PPI) was constructed. The found be associated various subcellular locations functional categories through Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis. It hypothesized interacting TGEV are mainly involved formation cytoplasmic translation initiation complex, mRNA binding, ribosomes, proteasomes. Among these, ATP5B, core subunit mitochondrial ATP synthase further studied. Coimmunoprecipitation (Co-IP) indirect immunofluorescence (IFA) results confirmed ATP5B. Furthermore, downregulation ATP5B expression promote suggesting might function negative regulator replication. Collectively, our offer additional insights into provide novel antiviral target against TGEV.

Язык: Английский

A Comprehensive View on the Protein Functions of Porcine Epidemic Diarrhea Virus DOI Open Access
Xin Li,

Yiwan Wu,

Zhibin Yan

и другие.

Genes, Год журнала: 2024, Номер 15(2), С. 165 - 165

Опубликована: Янв. 26, 2024

Porcine epidemic diarrhea (PED) virus (PEDV) is one of the main pathogens causing in piglets and fattening pigs. The clinical signs PED are vomiting, acute diarrhea, dehydration, mortality resulting significant economic losses becoming a major challenge pig industry. PEDV possesses various crucial structural functional proteins, which play important roles viral structure, infection, replication, assembly, release, as well escaping host innate immunity. Over past few years, there has been progress study pathogenesis, revealing role interaction between proteins cytokines infection. At present, control measure against vaccine immunization sows, but protective effect for emerging strains still insufficient, no ideal safe efficient vaccine. Although scientists have persistently delved their research into intricate structure functionalities genome pathogenic mechanism remains incompletely elucidated. Here, we focus on reviewing nonstructural to facilitate understanding biological processes such infection pathogenesis.

Язык: Английский

Процитировано

7
A Balancing Act: The Viral–Host Battle over RNA Binding Proteins DOI Creative Commons

Yahaira Bermudez,

David Hatfield,

Mandy Muller

и другие.

Viruses, Год журнала: 2024, Номер 16(3), С. 474 - 474

Опубликована: Март 20, 2024

A defining feature of a productive viral infection is the co-opting host cell resources for replication. Despite repertoire molecular functions and biological counter measures, viruses still subvert defenses to take control cellular factors such as RNA binding proteins (RBPs). RBPs are involved in virtually all steps mRNA life, forming ribonucleoprotein complexes (mRNPs) highly ordered regulated process fate stability cell. As such, hallmark takeover reshaping modulate gene expression evade immune responses by altering RBP interactions. Here, we provide an extensive review work this area, particularly on duality formation RNP that can be either pro- or antiviral. Overall, review, highlight various ways co-opt regulate outcome gathering novel insights gained from research studies field.

Язык: Английский

Процитировано

5
TOLLIP inhibits the replication of PEDV by autophagic degradation of Nsp9 DOI
Yahui Li, Yutao Zhang,

Jiexi Cheng

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140631 - 140631

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
POLM inhibits porcine epidemic diarrhea virus replication by degrading multiple viral structural proteins DOI Creative Commons
Xinyu Cao, Yingyu Liu, Tong Wu

и другие.

Journal of Virology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 10, 2025

Porcine epidemic diarrhea, as a porcine diarrhea virus (PEDV)-induced infectious intestinal condition typified by emesis, dehydration, and anorexia, leads to death rates high 100% among suckling piglets. Given the existing commercial vaccines, it is essential study host-virus interactions formulate efficient anti-viral regimes. This concerned host factor POLM (a DNA polymerase family member) that exerts an effect against PEDV proliferation. Our results indicated expression was increased following infection regulated transcription FOXA1. In addition, our findings targeted degraded structural proteins (N, S2, M) autophagy pathway inhibit could recruit E3 ubiquitination ligase MARCH8 for N, M protein ubiquitination, which subsequently recognized p62, cargo receptor, translocation autophagic lysosome, therefore degrading preventing summary, we showed novel therapeutic target combating PEDV, i.e., using POLM-MARCH8-p62-autophagosome degrade proteins.IMPORTANCEPEDV coronavirus causes mortality in piglets, poses significant economic damage swine farming. During infection, cells may promote natural immune response suppress viral replication through variety of potential factors. this study, found upregulation FOXA1 factor) during infection. It be new replication, interacted with (an ubiquitin ligase) p62 receptor) facilitate degradation via process. Apart from elucidating previously unidentified function POLM, also provides perspective studying factors act regulators anti-PEDV pathways.

Язык: Английский

Процитировано

0
Host restriction factors against porcine epidemic diarrhea virus: a mini-review DOI Creative Commons
Hongqing Zheng,

Chenhong Qiu,

Haolun Tian

и другие.

Veterinary Research, Год журнала: 2025, Номер 56(1)

Опубликована: Март 24, 2025

Abstract Porcine epidemic diarrhea is an acute contagious disease caused by porcine virus (PEDV), which severely constrains the development of global swine industry. Host restriction factors constitute a vital defensive barrier against viral infections, typically interacting with viruses at specific stages their replication process to disrupt it. Considering that traditional PEDV vaccines often struggle effectively activate mucosal immunity in sows and thereby fail provide reliable passive piglets via milk, this review focuses on host play crucial roles restricting infection replication. The aim identify potential targets for anti-PEDV drugs offer insights exploration novel vaccine adjuvants.

Язык: Английский

Процитировано

0
Encephalomyocarditis virus non-structural protein 2C induces the degradation of NDP52 autophagy protein to promote its own survival DOI

Rongqian Mo,

Rongrong Cheng, Ping Dong

и другие.

Veterinary Microbiology, Год журнала: 2025, Номер 306, С. 110549 - 110549

Опубликована: Май 8, 2025

Язык: Английский

Процитировано

0
Midnolin inhibits coronavirus proliferation by degrading viral proteins DOI Creative Commons
Y. F. Wang, Tong Wu,

Wenzhen Qin

и другие.

Journal of Virology, Год журнала: 2025, Номер unknown

Опубликована: Май 29, 2025

ABSTRACT Cells utilize proteasomes and selective autophagy to degrade ubiquitin-labeled viral proteins inhibit proliferation. Midnolin, a midbrain nucleolar protein, is reported use ubiquitin-independent proteasomal degradation various nuclear including transcription factors encoded by the immediate-early genes. However, it remains unclear whether midnolin can protein hinder replication. In this study, degraded porcine epidemic diarrhea virus (PEDV) S1/S2/M/E suppress PEDV Midnolin has two essential structural domains: Catch domain ubiquitin-like domain. We found that of concerted target both proteasome autophagy. Furthermore, study indicated individual through pathway, which recruited E3 ubiquitinating enzyme MARCH8 ubiquitinate proteins, ubiquitinated were recognized Tollip transported lysosome for degradation. Collectively, not only pathway but also revealed new mechanism midnolin-mediated restriction, where using (Catch)-MARCH8-Tollip-autophagosome pathway. IMPORTANCE Proteasomes are ways proliferation in cells. proteins. unknown. we During degradation, domains exerted vital role. The interact addition, Overall, have discovered acts as host factor antiviral function.

Язык: Английский

Процитировано

0
Nsp10-interacting host protein SAP18 restricts PEDV replication in Marc-145 cells via enhancing dephosphorylation of RIG-I DOI

Naling Yang,

Qi Zhang,

Quanqiong Wang

и другие.

Veterinary Microbiology, Год журнала: 2024, Номер 294, С. 110124 - 110124

Опубликована: Май 21, 2024

Язык: Английский

Процитировано

2
FSTL1 and TLR4 interact with PEDV structural proteins to promote virus adsorption to host cells DOI Creative Commons

Chunyun Liu,

Ning Kong, Hailong Liu

и другие.

Journal of Virology, Год журнала: 2024, Номер 99(1)

Опубликована: Дек. 13, 2024

Infection with porcine epidemic diarrhea virus (PEDV) results in enormous economic damage to the global swine industry. PEDV starts its life cycle by binding receptors of host cells and adsorbing onto cellular surfaces. However, it is still unknown how adsorbs surface mechanism beneath interplay cell transmembrane protein proteins. FSTL1, which a secreted glycoprotein, participates diverse pathological physiological processes, including immune modulation proliferation differentiation. The protein, TLR4, serves as pattern recognition receptor recognizing broad spectrum pathogens, exerts crucial effect on system. In this study, we identified that FSTL1 promoted infection. Further studies demonstrated interactive relationship between structural proteins (N S2). addition, also confirmed TLR4 interacted N, S1, S2 surface. Moreover, interaction induced viral adsorption cells. This study offers explicit evidence act mediators for interacting N/S proteins.IMPORTANCEAs highly infectious (PEDV)-induced intestinal condition swine, (PED) 100% death rate among suckling piglets poses serious burden farming. Therefore, essential investigate infection, replication, proliferation. Virus begins remains unclear revealed N proteins, while (N, thoroughly methodically was engaged internalization attachment processes promoting N\S

Язык: Английский

Процитировано

2
A Balancing Act: The Viral-Host Battle over RBPs DOI Open Access

Yahaira Bermudez,

David Hatfield,

Mandy Muller

и другие.

Опубликована: Дек. 18, 2023

A defining feature of a productive infection is the co-opting host cell resources for viral replication. Despite their repertoire molecular functions, viruses subvert defenses to take control cellular factors such as RNA binding proteins (RBPs). RBPs are involved in virtually all steps mRNA life forming ribonucleoprotein complexes (mRNPs) highly ordered and regulated process fate stability cell. Thus, hallmark this takeover reshape modulate gene expression evade immune responses. Here we provide an extensive review work area, particularly how host-viral interplay influences RBP functions Overall, review, highlight myriad ways can regulate either pro-viral or antiviral manner by gathering novel insights gained from research studies field.

Язык: Английский

Процитировано

1