Integration of Dynamical Network Biomarkers, Control Theory and Drosophila Model Identifies Vasa/DDX4 as the Potential Therapeutic Targets for Metabolic Syndrome

Cells, Год журнала: 2025, Номер 14(6), С. 415 - 415

Опубликована: Март 12, 2025

Metabolic syndrome (MetS) is a subclinical disease, resulting in increased risk of type 2 diabetes (T2D), cardiovascular diseases, cancer, and mortality. Dynamical network biomarkers (DNB) theory has been developed to provide early-warning signals the disease state during preclinical stage. To improve efficiency DNB analysis for target genes discovery, intervention based on control proposed. However, its biological validation specific such as MetS remains unexplored. Herein, we identified eight candidate from adipose tissue model mice at stage by analysis. Using Drosophila, conducted RNAi-mediated knockdown screening these vasa (also known DDX4), encoding DEAD-box RNA helicase, fat metabolism-associated gene. Fat body-specific abrogated high-fat diet (HFD)-induced enhancement starvation resistance through up-regulation triglyceride lipase. We also confirmed that DDX4 expressing adipocytes are HFD-fed high BMI patients using public datasets. These results prove potential search therapeutic targets diseases

Язык: Английский

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Mechanistic insights into impaired β-oxidation and its role in mitochondrial dysfunction: A comprehensive review

Diabetes Research and Clinical Practice, Год журнала: 2025, Номер unknown, С. 112129 - 112129

Опубликована: Март 1, 2025

Язык: Английский

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The Importance of Mitochondrial Processes in the Maturation and Acquisition of Competences of Oocytes and Embryo Culture

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4098 - 4098

Опубликована: Апрель 25, 2025

Mitochondria, as multifunctional and partially independent structures, play a crucial role in determining essential life processes. Recently, their significance reproductive biology has gained increasing attention. This review aims to comprehensively analyse the of mitochondrial processes oocyte maturation embryo culture. A comprehensive literature was conducted highlight importance activity early stages formation. Proper function provides energy, maintains genomic stability, ensures optimal conditions for fertilisation progression. Understanding these is optimise culture identify new biomarkers that improve success assisted technologies (ARTs). Enhancing female cells key improving quality, which can lead better vitro transfer. Furthermore, advances diagnostic techniques, such genome sequencing, offer more precise understanding relationship between health quality. However, fully functions only part challenge. Expanding knowledge interactions mitochondria other cellular structures future advancements medicine. complex relationships will provide deeper insight into outcomes development.

Язык: Английский

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The fetal origins of metabolic health: exploring the association between newborn biological age and metabolism hormones in childhood

BMC Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 8, 2024

Telomere length (TL), mitochondrial DNA copy number (mtDNAcn), and methylation age (DNAmAge) are common aging biomarkers. However, research on the associations between these three markers at birth subsequent metabolic status was limited. This study aimed to evaluate association TL, mtDNAcn, DNAmAge in newborns variation hormones of children 3 years old. involved 895 mother-child pairs from a cohort China, with TL mtDNAcn measured using quantitative real-time PCR, (DNAm) assessed Infinium MethylationEPIC Beadchip, DNAm determined Horvath's epigenetic clock. Insulin leptin levels were via electrochemiluminescence assay. Multivariable adjusted linear regression restricted cubic spline (RCS) analysis utilized examine hormones. The indicated percentage change metabolism for per doubling biomarkers alterations found significant insulin (adjusted percent (95% CI), - 13.22 (- 23.21 1.94)), 15.32 (1.32 31.24)), 14.13 21.59 5.95)). RCS revealed non-linear (Ln transformed) (P = 0.024 nonlinearity), as well 0.043 nonlinearity). Specifically, insulin, positive observed when less than 0.05, which transitioned an inverse greater 0.05. Regarding leptin, there sharp decline 1.35, followed by plateau 1.35 0.67 then further 0.67. In this prospective study, old associated birth. These findings suggested that might play role biological programming health

Язык: Английский

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Nitidine Chloride Alleviates Hypoxic Stress via PINK1-Parkin-Mediated Mitophagy in the Mammary Epithelial Cells of Milk Buffalo

Animals, Год журнала: 2024, Номер 14(20), С. 3016 - 3016

Опубликована: Окт. 18, 2024

Hypoxia in the mammary gland epithelial cells of milk buffalo (BMECs) can affect yield and composition, it even cause metabolic diseases. Nitidine chloride (NC) is a natural alkaloid with antioxidant properties that scavenge excessive reactive oxygen species (ROS). However, effect NC on hypoxic injury BMECs its molecular mechanisms are still unknown. Here, an immunofluorescence assay, transmission electron microscopy (TEM), flow cytometry, combined untargeted metabolomics, were used to investigate protective stress BMECs. It was found significantly reduce cell activity (p < 0.05) inhibit cellular oxidative apoptosis 0.05). A significant decrease mitophagy mediated by PINK1-Parkin pathway observed after pretreatment In addition, enrichment analysis demonstrated against may be related downregulation pathways involving aminoacyl tRNA biosynthesis; arginine proline metabolism; glycine, serine, threonine phenylalanine, tyrosine, tryptophan phenylalanine metabolism. Thus, has mitochondria, regulate amino acid metabolism response stress. The present study provides reference for application nitidine lactation function buffalo.

Язык: Английский

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Nörodejeneratif Hastalıklarda Yeni Bir Yaklaşım: Mitokondri Hedefli Tedavi

Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, Год журнала: 2024, Номер unknown

Опубликована: Авг. 12, 2024

Mitokondri hücrenin enerji metabolizmasında, oksidatif fosforilasyonda ve hücre ölümünde rol alan temel organeli olup pek çok hastalıkta olduğu gibi nörodejeneratif hastalıkların da patogenezine katkı sağlar. Günümüzde çoğu hastalığın etiyopatogenezinde reaktif oksijen nitrojen türlerine bağlı hasar sorumlu tutulmakla birlikte antioksidan tedaviler bu hasarın önlenmesi iyileştirilmesi konusunda yeterli olmamaktadır. Bu nedenle son yıllarda mitokondriyi direkt olarak hedefleyen içerisinde biriken farklı farmakolojik ajanlar geliştirilmiş birçok nörodejenerasyon hayvan modelinde iyileştirici etkileri görülmüştür. Aynı zamanda ajanlardan bazılarının insan klinik çalışmalarında güvenilir etkili kanıtlanmıştır. derlemede, Alzheimer Parkinson hastalıkları başta olmak üzere hastalıklarda mitokondri disfonksiyonu tedavisinde olacağı düşünülen hedefli tedavi ile ilgili güncel bilgiler tartışılmıştır.

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Identifying the key role of mitochondrial respiration and lipid metabolism in regulating axillary osmidrosis through proteomics analysis

Archives of Dermatological Research, Год журнала: 2024, Номер 317(1)

Опубликована: Дек. 14, 2024

Язык: Английский

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