Comprehensive analysis of DNA methylation and gene expression to identify tumor suppressor genes reactivated by MLN4924 in acute myeloid leukemia DOI
Yuancheng Guo,

Jinli Jian,

Xiaowei Tang

и другие.

Anti-Cancer Drugs, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

This study investigated whether the neddylation inhibitor MLN4924 induces aberrant DNA methylation patterns in acute myeloid leukemia and contributes to reactivation of tumor suppressor genes. profiles Kasumi-1 KU812 cell lines before after treatment were generated using 850K Methylation BeadChip. RNA sequencing was used obtain transcriptomic cells. Target genes identified through a combined analysis transcriptome data. Methylation-specific PCR quantitative validated changes expression. Prognostic target performed databases, Pearson correlation examine relationship between expression levels. In cells, 301 469 differentially methylated sites, respectively, identified. A total 4310 differential detected Kasumi-1. Combined revealed that TRIM58 exhibited significant demethylation upregulation treatment, as confirmed by methylation-specific PCR. Furthermore, database both down-expression promoter hypermethylation correlated with poor prognosis leukemia. negative observed suggests alters reactivates TRIM58, potential gene, demethylation.

Язык: Английский

Comprehensive analysis of DNA methylation and gene expression to identify tumor suppressor genes reactivated by MLN4924 in acute myeloid leukemia DOI
Yuancheng Guo,

Jinli Jian,

Xiaowei Tang

и другие.

Anti-Cancer Drugs, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

This study investigated whether the neddylation inhibitor MLN4924 induces aberrant DNA methylation patterns in acute myeloid leukemia and contributes to reactivation of tumor suppressor genes. profiles Kasumi-1 KU812 cell lines before after treatment were generated using 850K Methylation BeadChip. RNA sequencing was used obtain transcriptomic cells. Target genes identified through a combined analysis transcriptome data. Methylation-specific PCR quantitative validated changes expression. Prognostic target performed databases, Pearson correlation examine relationship between expression levels. In cells, 301 469 differentially methylated sites, respectively, identified. A total 4310 differential detected Kasumi-1. Combined revealed that TRIM58 exhibited significant demethylation upregulation treatment, as confirmed by methylation-specific PCR. Furthermore, database both down-expression promoter hypermethylation correlated with poor prognosis leukemia. negative observed suggests alters reactivates TRIM58, potential gene, demethylation.

Язык: Английский

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