International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3258 - 3258

Опубликована: Апрель 1, 2025

Experimental autoimmune encephalomyelitis (EAE) is a preclinical animal model widely used to study multiple sclerosis (MS). Blood-based analytes, including cytokines and neural biomarkers are the predictors of neurodegeneration, disease activity, disability in patients with MS. However, understudied confounding factors cause variation reports on EAE across strains/studies, limiting utility these for predicting activity. In this study, we investigated blood-based analyte profiles, markers (NFL GFAP) (IL-6, IL-17, IL-12p70, IL-10, TNF-α), two clinically distinct models: relapsing-remitting (RR)-EAE chronic-EAE. Ultrasensitive single-molecule array technology (SIMOA, Quanterix) was profile analytes blood plasma mice at acute, chronic, progressive phases disease. both models, NFL substantially increased during post-disease onset all phases, pronounced increase observed The leakage GFAP into peripheral also greater after especially acute phase Among cytokines, only IL-10 had consistently lower levels models throughout course This suggests NFL, GFAP, as potential translational activity EAE, making them candidates surrogate testing therapeutic interventions

Язык: Английский