Separations, Год журнала: 2025, Номер 12(2), С. 53 - 53
Опубликована: Фев. 18, 2025
Trimethylamine-N-oxide (TMAO) is associated with various chronic diseases. TMAO a downstream oxidative metabolite of trimethylamine (TMA) that generated by the gut microbiota from dietary choline, carnitine, and betaine. Current analytical methods predominantly target only, due to challenge efficiently extracting quantifying TMA. The present study demonstrates simple rapid UPLC–MRM–MS method for concurrent quantification TMAO, TMA, related precursors (choline, betaine, carnitines) following methanol extraction plasma derivatization using iodoacetonitrile (IACN). Pure resulted in higher extractability TMA (up two-fold) compared both pure acetonitrile methanol/acetonitrile mixtures. showed high linearity within tested range 0.0625–100 μmol/L (determination coefficient > 0.999) an intra- (n = 3) inter-day 9) precision 2–8% along average recovery above 94% all metabolites (TMAO, L-carnitine, acetyl-L-carnitine, propionyl-L-carnitine). method’s applicability was confirmed through comparison its precursor concentrations samples overnight-fasted subjects 12) without 21) metabolic syndrome.
Язык: Английский
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0
RSC Advances, Год журнала: 2025, Номер 15(11), С. 8707 - 8718
Опубликована: Янв. 1, 2025
Biomarkers like creatinine (CRE) and calcium ions (Ca 2+ ) are vital for detecting several disorders chronic kidney disease (CKD).
Язык: Английский
Процитировано
0
Clinica Chimica Acta, Год журнала: 2025, Номер 573, С. 120294 - 120294
Опубликована: Апрель 11, 2025
Язык: Английский
Процитировано
0
Journal of Chromatography B, Год журнала: 2025, Номер unknown, С. 124602 - 124602
Опубликована: Апрель 1, 2025
Dimethylamine (DMA) and its relatives including trimethylamine (TMA) N-oxide (TMAO) are widely distributed in nature humans the marine ecosystem. This article gives an overview of origin, occurrence, metabolism potential functions dimethylamine human life DMA-TMA-TMAO axis In humans, a fraction about 80-90 % is endogenous origin. The remaining 10-20 exogenous notably foods especially certain fish seafood. Several different analytical methods available for quantitative determination dimethylamine, biological samples urine fish. Frequently used include GC-FID, GC-ECD, GC-MS more recently LC-MS/MS. A method includes extractive derivatization with pentafluorobenzoyl chloride. concentration plasma serum oder 3 μM healthy several times higher suffering from chronic kidney diseases. may range between 100 1500 μM, corresponding to mean creatinine-corrected excretion rates 10 80 μmol dimethylamine/mmol creatinine adults up 400 children adolescents. have contributed greatly better understanding biology health disease. 90 urinary considered measure synthesis post-translational dimethylation arginine residues proteins. abundant modification leads asymmetrically dimethylated proteins, which, upon regular proteolysis, generate asymmetrical dimethylarginine (ADMA) that finally hydrolyzed L-citrulline which then readily excreted urine. fishery, discussed as indicators freshness due their enhanced production microbiomal activity. High amounts found canned precursor cancerogenic N-nitroso-dimethylamine. Other non-volatile amines such histamine agmatine seem be suitable indicators. contents order mmol per kg tissue dependence on habitat depth. It assumed act osmolytic piezolytic agent. significance disease elusive.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0