Abstract
Genetic
testing
plays
an
important
role
in
human
health
management
and
disease
prevention.
However,
traditional
genetic
methods
have
been
unable
to
fulfill
the
current
diagnostic
needs
owing
several
limitations,
including
high
cost,
complexity,
difficulty
performing.
Smartphones
multiple
inherent
advantages,
such
as
easy
portability,
ubiquity,
fast
processing
speed,
excellent
imaging
capabilities,
enormous
potential
realizing
rapid
on‐site
testing.
The
present
review
documents
research
progress
of
smartphone‐based
optical
biosensors
fields
colorimetry,
fluorescence,
microscopic
for
Furthermore,
describes
their
applications
diagnostics,
which
range
from
infectious
diseases
hereditary
cancers.
Finally,
challenges
perspectives
regarding
are
discussed.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 5, 2024
The
CRISPR
(clustered
regularly
interspaced
short
palindromic
repeats)/Cas
(CRISPR
associated)
system
has
proven
to
be
a
powerful
tool
for
nucleic
acid
detection
due
its
inherent
advantages
of
effective
identification
and
editing
capabilities,
is
therefore
known
as
the
next-generation
molecular
diagnostic
technology.
However,
technologies
based
on
CRISPR/Cas
systems
require
preamplification
target
analytes;
that
is,
gene
amplification
steps
through
isothermal
or
PCR
before
increase
analyte
concentrations.
This
creates
number
testing
limitations,
such
extended
time
need
more
sophisticated
instruments.
To
overcome
above
various
amplification-free
assay
strategies
have
been
explored
alternatives,
which
omit
step
concentrations
analytes.
Nanozymes
play
pivotal
role
in
enhancing
sensitivity
CRISPR-based
detection,
enabling
visual
rapid
assays.
utilization
nanozyme
exceptional
enzyme-like
catalytic
activity
holds
great
promise
signal
both
electrochemical
optical
domains,
encompassing
sensors
colorimetric
sensors.
Rather
than
relying
converting
single
into
multiple
analytes,
these
methods
focus
amplification,
main
mechanism
involves
ability
form
large
reporter
molecules
improve
performance
sensor.
exploitation
nanozymes
results
heightened
accuracy
outcomes.
In
addition
sensor
application
nanozymes,
additional
are
needed
achieve
without
processes.
Herein,
we
review
improving
do
not
preamplification,
providing
simple,
intuitive
preamplification-free
platform
by
in-system
one-step
programs,
nanozyme-mediated
strategies.
Abstract
Genetic
testing
plays
an
important
role
in
human
health
management
and
disease
prevention.
However,
traditional
genetic
methods
have
been
unable
to
fulfill
the
current
diagnostic
needs
owing
several
limitations,
including
high
cost,
complexity,
difficulty
performing.
Smartphones
multiple
inherent
advantages,
such
as
easy
portability,
ubiquity,
fast
processing
speed,
excellent
imaging
capabilities,
enormous
potential
realizing
rapid
on‐site
testing.
The
present
review
documents
research
progress
of
smartphone‐based
optical
biosensors
fields
colorimetry,
fluorescence,
microscopic
for
Furthermore,
describes
their
applications
diagnostics,
which
range
from
infectious
diseases
hereditary
cancers.
Finally,
challenges
perspectives
regarding
are
discussed.
