One‐pot immobilization of formate dehydrogenase on silica nanoparticles based on catechol chemistry with or without polyethyleneimine DOI
Ruqing Chong,

Lingding Meng,

Qiyong Liao

и другие.

Journal of Applied Polymer Science, Год журнала: 2024, Номер 141(32)

Опубликована: Май 29, 2024

Abstract Converting carbon dioxide into formic acid with formate dehydrogenase (FDH) as a catalyst is gentle and eco‐friendly process that not only conserves energy reduces emissions but transforms waste valuable resource. Enzyme immobilization common strategy for improving the stability recyclability of enzyme, however, procedures are usually complicated include surface modification binding reactions in order to form reliable linkages. Co‐deposition polydopamine (PDA) polyethyleneimine (PEI) popular approach versatile fields, their co‐deposition enzymes rarely reported. Herein, one‐pot FDH on SiO 2 nanoparticles based catechol chemistry was attempted first time or without PEI, resultant immobilized enzymes, FDH/PDA/PEI‐SiO FDH/PDA‐SiO , were characterized, effects composition deposition conditions catalytic performance investigated. exhibited higher apparent specific activity than which separately 0.167 0.136 mM/h/mg. The recovery rate 464% 378% free enzyme. After 5 cycles, relative 82.3% 87.4%. This research significant presenting simple high‐efficient method can be extended other kinds enzymes.

Язык: Английский

Biocatalysis-Driven CO2 Valorization: Innovations and Sustainable Strategies in Conversion and Utilization DOI Creative Commons
Lin Yuan, Emmanuel Mintah Bonku, Zhonghua Yang

и другие.

Carbon Capture Science & Technology, Год журнала: 2025, Номер unknown, С. 100437 - 100437

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
One‐pot immobilization of formate dehydrogenase on silica nanoparticles based on catechol chemistry with or without polyethyleneimine DOI
Ruqing Chong,

Lingding Meng,

Qiyong Liao

и другие.

Journal of Applied Polymer Science, Год журнала: 2024, Номер 141(32)

Опубликована: Май 29, 2024

Abstract Converting carbon dioxide into formic acid with formate dehydrogenase (FDH) as a catalyst is gentle and eco‐friendly process that not only conserves energy reduces emissions but transforms waste valuable resource. Enzyme immobilization common strategy for improving the stability recyclability of enzyme, however, procedures are usually complicated include surface modification binding reactions in order to form reliable linkages. Co‐deposition polydopamine (PDA) polyethyleneimine (PEI) popular approach versatile fields, their co‐deposition enzymes rarely reported. Herein, one‐pot FDH on SiO 2 nanoparticles based catechol chemistry was attempted first time or without PEI, resultant immobilized enzymes, FDH/PDA/PEI‐SiO FDH/PDA‐SiO , were characterized, effects composition deposition conditions catalytic performance investigated. exhibited higher apparent specific activity than which separately 0.167 0.136 mM/h/mg. The recovery rate 464% 378% free enzyme. After 5 cycles, relative 82.3% 87.4%. This research significant presenting simple high‐efficient method can be extended other kinds enzymes.

Язык: Английский

Процитировано

1