Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Июнь 8, 2023
Extracellular
vesicles
(EVs)
have
emerged
as
a
promising
platform
for
gene
delivery
owing
to
their
natural
properties
and
phenomenal
functions,
being
able
circumvent
the
significant
challenges
associated
with
toxicity,
problematic
biocompatibility,
immunogenicity
of
standard
approaches.
These
features
are
particularly
interest
targeted
emerging
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)/CRISPR-associated
(Cas)
systems.
However,
current
efficiency
EV-meditated
transport
CRISPR/Cas
components
remains
insufficient
due
numerous
exogenous
endogenous
barriers.
Here,
we
comprehensively
reviewed
status
EV-based
In
particular,
explored
various
strategies
methodologies
available
potentially
improve
loading
capacity,
safety,
stability,
targeting,
tracking
system
delivery.
Additionally,
hypothesise
future
avenues
development
systems
that
could
pave
way
novel
clinically
valuable
approaches,
may
bridge
gap
between
editing
technologies
laboratory/clinical
application
therapies.
Aging and Disease,
Год журнала:
2024,
Номер
unknown, С. 0 - 0
Опубликована: Янв. 1, 2024
Nanomaterials
(NMs)
have
emerged
as
promising
tools
for
disease
diagnosis
and
therapy
due
to
their
unique
physicochemical
properties.
To
maximize
the
effectiveness
design
of
NMs-based
medical
applications,
it
is
essential
comprehend
complex
mechanisms
cellular
uptake,
subcellular
localization,
retention.
This
review
illuminates
various
pathways
that
NMs
take
get
from
extracellular
environment
certain
intracellular
compartments
by
investigating
underlie
interaction
with
cells.
The
uptake
involves
interactions
cell
membranes,
encompassing
endocytosis,
phagocytosis,
other
active
transport
mechanisms.
Unique
patterns
across
types
highlight
necessity
customized
designs.
After
internalization,
move
through
a
variety
routes
affect
where
they
are
located
subcellularly.
Understanding
these
pivotal
enhancing
targeted
delivery
therapeutic
agents
imaging
probes.
Furthermore,
retention
plays
critical
role
in
sustained
efficacy
long-term
capabilities.
Factors
influencing
include
nanoparticle
size,
surface
chemistry,
microenvironment.
Strategies
prolonging
discussed,
including
modifications
encapsulation
techniques.
In
conclusion,
comprehensive
understanding
governing
advancing
application
therapy.
provides
insights
into
intricate
interplay
between
biological
systems,
offering
foundation
rational
next-generation
nanomedicines.
Frontiers in Immunology,
Год журнала:
2022,
Номер
13
Опубликована: Дек. 9, 2022
Sepsis
is
a
life-threatening
organ
dysfunction
resulting
from
dysregulated
host
responses
to
infection.
Macrophages
play
significant
roles
in
against
pathogens
and
the
immunopathogenesis
of
sepsis,
such
as
phagocytosis
pathogens,
secretion
cytokines,
phenotype
reprogramming.
However,
rapid
progression
sepsis
impairs
macrophage
function,
conventional
antimicrobial
supportive
treatment
are
not
sufficient
restore
macrophages
roles.
Nanoparticles
own
unique
physicochemical
properties,
surface
functions,
localized
plasmon
resonance
phenomenon,
passive
targeting
vivo,
good
biocompatibility
biodegradability,
accessible
for
biomedical
applications.
Once
into
body,
NPs
recognized
by
immune
system.
phagocytes
innate
immunity
dedicated
recognition
foreign
substances,
including
nanoparticles,
with
which
an
response
subsequently
occurs.
Various
design
strategies,
functionalization,
have
been
implemented
manipulate
nanoparticles
monocytes/macrophages,
engulfed
them
regulate
their
function
compensating
shortcomings
traditional
methods.
The
review
summarizes
mechanism
nanomaterials
recent
advances
nanomedicine
provides
insight
exploring
macrophage-based
nano-management
sepsis.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Июнь 8, 2023
Extracellular
vesicles
(EVs)
have
emerged
as
a
promising
platform
for
gene
delivery
owing
to
their
natural
properties
and
phenomenal
functions,
being
able
circumvent
the
significant
challenges
associated
with
toxicity,
problematic
biocompatibility,
immunogenicity
of
standard
approaches.
These
features
are
particularly
interest
targeted
emerging
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)/CRISPR-associated
(Cas)
systems.
However,
current
efficiency
EV-meditated
transport
CRISPR/Cas
components
remains
insufficient
due
numerous
exogenous
endogenous
barriers.
Here,
we
comprehensively
reviewed
status
EV-based
In
particular,
explored
various
strategies
methodologies
available
potentially
improve
loading
capacity,
safety,
stability,
targeting,
tracking
system
delivery.
Additionally,
hypothesise
future
avenues
development
systems
that
could
pave
way
novel
clinically
valuable
approaches,
may
bridge
gap
between
editing
technologies
laboratory/clinical
application
therapies.
