Journal of Periodontal Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Periodontal osseous defects are mainly caused by periodontitis, which seriously affects the quality of patient life. Dental pulp cells (DpCs)-derived extracellular vesicles (EVs) can effectively promote tissue regeneration. Homeobox A9 (HOXA9) mRNA is abundant in EVs derived from DSCs, may be related to promoting alveolar bone regeneration, but specific mechanism unclear. We aimed elucidate through HOXA9 DPCs-derived impact osteogenic differentiation periodontal ligament (PDLCs). were isolated and characterized transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blot. Lipopolysaccharide (LPS) was employed induce inflammatory environment. Cell viability assessed CCK8 assay. Calcium deposition determined Alizarin red staining. H3K27ac enrichment FLI1 enhancer region interaction between C/EBPα, HOXA9, analyzed ChIP The 293T dual luciferase reporter gene promoted PDLC osteogenesis under LPS treatment increased expression PDLCs. knockdown DPCs reversed effect on differentiation. facilitating competitively binding with C/EBPα. Moreover, activating PI3K/AKT pathway upregulating FLI1. Our study identified a previously unknown that HOXA9/FLI1 signaling axis participates processes treat injury. research presents theoretical basis for using

Язык: Английский