Extracellular Vesicles From Dental Pulp Cells Promote Osteogenic Differentiation in Periodontal Ligament Cells DOI Open Access
Yingying Li, Fenglan Li, Haitao Liu

и другие.

Journal of Periodontal Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Periodontal osseous defects are mainly caused by periodontitis, which seriously affects the quality of patient life. Dental pulp cells (DpCs)-derived extracellular vesicles (EVs) can effectively promote tissue regeneration. Homeobox A9 (HOXA9) mRNA is abundant in EVs derived from DSCs, may be related to promoting alveolar bone regeneration, but specific mechanism unclear. We aimed elucidate through HOXA9 DPCs-derived impact osteogenic differentiation periodontal ligament (PDLCs). were isolated and characterized transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blot. Lipopolysaccharide (LPS) was employed induce inflammatory environment. Cell viability assessed CCK8 assay. Calcium deposition determined Alizarin red staining. H3K27ac enrichment FLI1 enhancer region interaction between C/EBPα, HOXA9, analyzed ChIP The 293T dual luciferase reporter gene promoted PDLC osteogenesis under LPS treatment increased expression PDLCs. knockdown DPCs reversed effect on differentiation. facilitating competitively binding with C/EBPα. Moreover, activating PI3K/AKT pathway upregulating FLI1. Our study identified a previously unknown that HOXA9/FLI1 signaling axis participates processes treat injury. research presents theoretical basis for using

Язык: Английский

Unlocking Exosome Therapeutics: The Critical Role of Pharmacokinetics in Clinical Applications DOI
Krishna Yadav, Kantrol Kumar Sahu,

Sucheta

и другие.

Tissue and Cell, Год журнала: 2025, Номер 93, С. 102749 - 102749

Опубликована: Янв. 23, 2025

Язык: Английский

Процитировано

0

Exposure to Group B Streptococcus-induced chorioamnionitis alters the proteome of placental extracellular vesicles DOI

Seline Vancolen,

Mathilde Chevin, Bernard Robaire

и другие.

Placenta, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Extracellular vesicles as drug and gene delivery vehicles in central nervous system diseases DOI Creative Commons

Xi Shi,

Weilong He,

Ashwin Gupta

и другие.

Biomaterials Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

This review summarizes naïve extracellular vesicles (EVs) in clinical trials for central nervous system (CNS) diseases and updates recent translational preclinical research on EV-loaded drugs or genes CNS treatments.

Язык: Английский

Процитировано

0

Nanotechnology in Parkinson’s Disease: overcoming drug delivery challenges and enhancing therapeutic outcomes DOI
Irfan Ali,

Mohammad Adil,

Mohammad Imran

и другие.

Drug Delivery and Translational Research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Язык: Английский

Процитировано

0

Extracellular Vesicles From Dental Pulp Cells Promote Osteogenic Differentiation in Periodontal Ligament Cells DOI Open Access
Yingying Li, Fenglan Li, Haitao Liu

и другие.

Journal of Periodontal Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Periodontal osseous defects are mainly caused by periodontitis, which seriously affects the quality of patient life. Dental pulp cells (DpCs)-derived extracellular vesicles (EVs) can effectively promote tissue regeneration. Homeobox A9 (HOXA9) mRNA is abundant in EVs derived from DSCs, may be related to promoting alveolar bone regeneration, but specific mechanism unclear. We aimed elucidate through HOXA9 DPCs-derived impact osteogenic differentiation periodontal ligament (PDLCs). were isolated and characterized transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blot. Lipopolysaccharide (LPS) was employed induce inflammatory environment. Cell viability assessed CCK8 assay. Calcium deposition determined Alizarin red staining. H3K27ac enrichment FLI1 enhancer region interaction between C/EBPα, HOXA9, analyzed ChIP The 293T dual luciferase reporter gene promoted PDLC osteogenesis under LPS treatment increased expression PDLCs. knockdown DPCs reversed effect on differentiation. facilitating competitively binding with C/EBPα. Moreover, activating PI3K/AKT pathway upregulating FLI1. Our study identified a previously unknown that HOXA9/FLI1 signaling axis participates processes treat injury. research presents theoretical basis for using

Язык: Английский

Процитировано

0