Therapeutic Applications of Aptamers

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6742 - 6742

Опубликована: Июнь 19, 2024

Affinity reagents, or target-binding molecules, are quite versatile and major workhorses in molecular biology medicine. Antibodies the most famous frequently used type they have been for a wide range of applications, including laboratory techniques, diagnostics, therapeutics. However, antibodies not only available affinity reagents do significant drawbacks, laborious costly production. Aptamers one potential alternative that variety unique advantages. They single stranded DNA RNA molecules can be selected binding to many targets proteins, carbohydrates, small molecules-for which typically low affinity. There also cost-effective methods producing modifying nucleic acids vitro without cells, whereas require cells even whole animals. While there drawbacks using aptamers therapeutic vivo stability, had success clinical trials treating diseases two aptamer-based drugs gained FDA approval. Aptamer development is still ongoing, could lead additional applications aptamer therapeutics, antitoxins, combinatorial approaches with nanoparticles other acid therapeutics improve efficacy.

Язык: Английский

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Therapeutic Applications of Aptamers

Опубликована: Май 22, 2024

Affinity reagents, or target-binding molecules, are quite versatile and major workhorses in molecular biology medicine. Antibodies the most famous frequently used type they have been for a wide range of applications, including laboratory techniques, diagnostics, therapeutics. However, antibodies not only available affinity reagents do significant drawbacks, laborious costly production. Aptamers one potential alternative that variety unique advantages. They single stranded DNA RNA molecules can be selected binding to many targets proteins, carbohydrates, small – which typically low affinity. There also cost-effective methods producing modifying nucleic acids vitro without cells, whereas require cells even whole animals. While there drawbacks using aptamers therapeutic vivo stability, had success clinical trials treating diseases two aptamer-based drugs gained FDA approval. Aptamer development is still ongoing, could lead additional applications aptamer therapeutics, antitoxins, combinatorial approaches with nanoparticles other acid therapeutics improve efficacy.

Язык: Английский

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Antimicrobial Resistance in Malaysian Shrimp Aquaculture and Strategies to Reduce Its Occurrence

Reviews in Aquaculture, Год журнала: 2025, Номер 17(3)

Опубликована: Апрель 2, 2025

ABSTRACT Shrimp is a commercially important species in several regions and among the key global aquaculture commodities essential for food production security. Similar to most shrimp‐producing countries, shrimp Malaysia suffers from recurring disease outbreaks that consequently impact overall economy. The use of antimicrobial agents, particularly antibiotics, prophylactic treatment growth enhancement has increased spread antimicrobial‐resistant bacteria aquatic environment. development dissemination other potential sources contamination waterways are facilitated by continuous application antibiotics farming, municipalities, livestock, hospitals pharmaceutical sources. This situation contributes resistance (AMR) phenomenon, One Health issue with detrimental effects on human animal health as well Addressing risks associated AMR remains highly challenging due intensification farming trends, which heightens outbreaks, limited availability alternatives many farmers seeking prevent crop failure. In this review, we critically examine issues related explore emerging strategies. Our analysis encompasses comprehensive review existing literature Malaysia, alternative mitigation strategies aimed at fostering more sustainable resilient practices.

Язык: Английский

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Therapeutic Potential of Insect Defensin DLP4 Against Staphylococcus hyicus-Infected Piglet Exudative Epidermitis

Pharmaceutics, Год журнала: 2024, Номер 16(11), С. 1350 - 1350

Опубликована: Окт. 22, 2024

: The emergence of resistance to

Язык: Английский

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Selection of streptococcal glucan-binding protein C specific DNA aptamers to inhibit biofilm formation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 23, 2024

Abstract Streptococcus mutans is a commensal bacterium in the oral cavity, but its overgrowth leads to biofilm formation and dental caries. S. glucan-binding protein C (GbpC) cornerstone of scaffold thus rational target for inhibition. Here we present selection DNA aptamers GbpC aid development inhibiting drugs. During SELEX, used extracellular domain as structurally homologous antigen I/II GbpC-deficient strain counter-targets. The aptamer candidates obtained were panned using method based on primer-blocked asymmetric PCR AlphaScreen. interaction between most promising panning was analysed by biolayer interferometry microscale thermophoresis. Finally, tested inhibitory effect wild-type . measurements demonstrated both efficacy selectivity aptamers. Two studied reduced 30% case strains no reduction detected strain, confirming suitability strategy presented providing lead molecules inhibitors.

Язык: Английский

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Selection of streptococcal glucan-binding protein C specific DNA aptamers to inhibit biofilm formation

International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 138579 - 138579

Опубликована: Дек. 1, 2024

Язык: Английский

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