Programmable DNA‐Peptide Conjugated Hydrogel via Click Chemistry for Sequential Modulation of Peripheral Nerve Regeneration
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 23, 2025
Abstract
During
peripheral
nerve
regeneration,
current
deoxyribonucleic
acid
(DNA)‐based
therapeutic
platforms
face
the
challenge
of
precisely
regulating
Schwann
cells
(SCs)
fate
to
sustain
their
repair
phenotype
due
inability
stably
and
integrate
multiple
bioactive
components.
Herein,
strain‐promoted
azide–alkyne
cycloaddition
reaction
is
utilized
neurotrophic
factor
mimetic
peptide
RGI
laminin‐derived
IKVAV
into
DNA
monomers.
Through
sequence
self‐assembly,
a
programmable
DNA‐peptide
conjugated
hydrogel
constructed
for
loading
bone
marrow
mesenchymal
stem
cell‐derived
exosomes.
This
can
rapidly,
stably,
various
components
network,
thereby
enabling
sequential
modulation
repair.
In
vitro,
studies
show
that
this
hydrogel,
through
mechanisms,
activate
neuregulin‐1
(Nrg1)/ErbB
pathway
induce
reprogramming
SCs
promote
recruitment
proliferation
SCs.
The
induced
neuronal
axon
outgrowth
enhance
tube
formation
in
endothelial
cells.
vivo,
gelate
situ
intraneural
injection
rat
sciatic
crush
injury
model,
promoting
regeneration
functional
recovery.
summary,
work
provides
an
effective
practical
strategy
regeneration.
Язык: Английский
The Potential Role of Adipose-Derived Stem Cells in Regeneration of Peripheral Nerves
Neurology International,
Год журнала:
2025,
Номер
17(2), С. 23 - 23
Опубликована: Фев. 6, 2025
Peripheral
nerve
injuries
are
common
complications
in
surgical
and
dental
practices,
often
resulting
functional
deficiencies
reduced
quality
of
life.
Current
treatment
choices,
such
as
autografts,
have
limitations,
including
donor
site
morbidity
suboptimal
outcomes.
Adipose-derived
stem
cells
(ADSCs)
shown
assuring
regenerative
potential
due
to
their
accessibility,
ease
harvesting
propagation,
multipotent
properties.
This
review
investigates
the
therapeutic
ADSCs
peripheral
regeneration,
focusing
on
use
bioengineered
conduits
supportive
microenvironments.
The
analysis
is
constructed
published
case
reports,
organized
reviews,
clinical
trials
from
Phase
I
III
that
investigate
managing
injuries,
emphasizing
both
orofacial
applications.
findings
highlight
advantages
promoting
secretion
angiogenic
neurotrophic
factors,
support
for
cellular
persistence,
supplementing
scaffold-based
tissue
repair.
capabilities
offer
a
novel
approach
augmenting
repair
recovery.
accessibility
adipose
minimally
invasive
nature
ADSC
further
encourage
its
prospective
application
an
autologous
cell
source
medicine.
Future
research
needed
ascertain
standardized
protocols
optimize
outcomes,
paving
way
become
mainstay
regeneration.
Язык: Английский
A comprehensive review of hydrogel strategies for repairing peripheral nerve injuries
Brain‐X,
Год журнала:
2025,
Номер
3(1)
Опубликована: Фев. 8, 2025
Abstract
As
an
etiological
factor
underlying
physical
and
mental
disability
in
humans,
peripheral
nerve
injuries
(PNIs)
can
induce
pain,
sensory
impairment,
disability.
Despite
their
regenerative
ability,
nerves
cannot
self‐repair
after
severe
defects.
While
grafting
is
the
gold
standard
for
treatment
of
PNIs,
it
limited
by
graft
versus
host
reactions,
surgical
complications,
donor
nerves.
field
material
science
continues
to
develop,
hydrogels
have
been
proposed
use
PNI
repair
biomodification,
targeted
modification,
or
loading
with
biological
factors
cells.
This
article
reviewed
research
advances
used
repair,
including
simple
composite
functionalized
loaded
Based
on
findings
from
these
reviews,
we
determined
that
further
clarification
mechanisms
action
modulating
cellular
functions
necessary.
In
addition,
there
a
need
explore
synergistic
effect
novel
other
biological,
physical,
biochemical
factors.
clinical
trials
are
still
limited,
scientific
efforts
expected
promote
application
repair.
Язык: Английский
The application of stem cells and exosomes in promoting nerve conduits for peripheral nerve repair
Biomaterials Research,
Год журнала:
2025,
Номер
29
Опубликована: Янв. 1, 2025
The
repair
of
peripheral
nerve
injury
(PNI)
presents
a
multifaceted
and
protracted
challenge,
with
current
therapeutic
approaches
failing
to
achieve
optimal
outcomes,
thereby
not
satisfying
the
considerable
clinical
demand.
advent
tissue
engineering
has
led
growing
body
experimental
evidence
indicating
that
synergistic
application
conduits,
which
provide
structural
guidance,
alongside
biological
signals
derived
from
exosomes
stem
cells,
yields
superior
results
for
PNI
compared
isolated
interventions.
This
combined
approach
holds
great
promise
application.
In
this
review,
we
present
latest
advancements
in
treatment
through
integration
cells
or
conduits.
We
have
addressed
inadequate
efficiency
conjunction
conduits
3
perspectives:
enhancing
exosomes,
improving
incorporating
physical
stimulation.
Язык: Английский
In Vivo Tracking of Amniotic Fluid Derived Stem Cells on Acellular Nerve Graft
Xue Ma,
Kaitlin Henry,
Kelsey M. Healy
и другие.
The Journal Of Hand Surgery,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 1, 2025
Язык: Английский
Injectable decellularized Wharton's jelly hydrogel containing CD56+ umbilical cord mesenchymal stem cell-derived exosomes for meniscus tear healing and cartilage protection
Materials Today Bio,
Год журнала:
2024,
Номер
29, С. 101258 - 101258
Опубликована: Сен. 19, 2024
Язык: Английский
Advanced techniques and innovations in peripheral nerve repair: a comprehensive review for clinical and experimental reference
Reviews in the Neurosciences,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 19, 2024
Abstract
Peripheral
nerve
injury,
resulting
from
various
physical
and
chemical
causes,
has
a
high
incidence
significant
functional
impact.
This
affecting
both
sensory
motor
functions,
can
severely
diminish
quality
of
life
cause
mental
health
issues.
Consequently,
it
is
major
focus
current
research.
Recent
advancements
in
peripheral
repair
technology,
including
the
application
new
techniques
materials,
have
expanded
options
for
methods.
A
comprehensive
article
that
combines
pathological
process
with
these
methods
needed
to
advance
research
this
field.
review
aims
provide
overview
repairing
injuries.
Beginning
histopathology
evaluates
detail
offer
clinical
guidance.
summarizes
advantages
disadvantages
methods,
photobiological
modulation
therapy,
suture
repair,
graft
vein
catheter
muscle
laser
welding
sliding
growth
factor-assisted
stem
cell
exosome
therapy.
Additionally,
explores
future
directions
treatment
injuries,
providing
valuable
references
experimental
treatment.
Язык: Английский
Extracellular vesicle therapy in neurological disorders
Journal of Biomedical Science,
Год журнала:
2024,
Номер
31(1)
Опубликована: Авг. 25, 2024
Abstract
Extracellular
vesicles
(EVs)
are
vital
for
cell-to-cell
communication,
transferring
proteins,
lipids,
and
nucleic
acids
in
various
physiological
pathological
processes.
They
play
crucial
roles
immune
modulation
tissue
regeneration
but
also
involved
pathogenic
conditions
like
inflammation
degenerative
disorders.
EVs
have
heterogeneous
populations
cargo,
with
numerous
subpopulations
currently
under
investigations.
EV
therapy
shows
promise
stimulating
repair
serving
as
a
drug
delivery
vehicle,
offering
advantages
over
cell
therapy,
such
ease
of
engineering
minimal
risk
tumorigenesis.
However,
challenges
remain,
including
inconsistent
nomenclature,
complex
characterization,
underdeveloped
large-scale
production
protocols.
This
review
highlights
the
recent
advances
significance
heterogeneity,
emphasizing
need
better
understanding
their
disease
pathologies
to
develop
tailored
therapies
clinical
applications
neurological
Graphical
abstract
Язык: Английский