Materials chemistry horizons., Год журнала: 2024, Номер 3(2), С. 1 - 14
Опубликована: Ноя. 1, 2024
Язык: Английский
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Янв. 4, 2025
Abstract Chondrocyte senescence is an important pathogenic factor causing osteoarthritis (OA) progression through persistently producing pro‐inflammatory factors. Mesenchymal stem cells‐derived small extracellular vesicles (MSC‐sEVs) have shown anti‐inflammatory effects in OA models, while persistent existence of senescent chondrocytes still promotes cartilage destruction. Therefore, improving the targeted elimination ability on required to facilitate translation MSC‐sEVs treatment. In this study, versatile engineered are developed targetedly clear and maintain metabolic homeostasis. Specifically, loaded with siRNA mouse double minute 2 homologue (siMDM2) modified cartilage‐targeting peptide WYRGRL‐PEG 2K ‐DSPE (WPD), named WPD‐sEVs siMDM2 . The results demonstrate modification improves cellular uptake chondrocytes, thus antiaging effects. Importantly, multifunctional enhances penetration extends joint retention time MSC‐sEVs. both post‐traumatic mice naturally aged mice, more effectively eliminates maintained matrix By using P53 phosphorylation inhibitor, essential role MDM2‐P53 pathway function verified. ex vivo cultured human explants, it confirmed that alleviates phenotype. Altogether, findings suggest promising translational potential for
Язык: Английский
Процитировано
1
Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер 106, С. 106696 - 106696
Опубликована: Фев. 6, 2025
Язык: Английский
Процитировано
1
Life Sciences, Год журнала: 2025, Номер 368, С. 123499 - 123499
Опубликована: Фев. 22, 2025
Язык: Английский
Процитировано
1
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive cargo and displaying diverse surface components that reflect their cellular origins functions. The EV surface, composed proteins, lipids, glycocalyx elements, plays a pivotal role in targeting recipient cells, mediating biological interactions, enabling selective delivery. This review comprehensively examined the molecular architecture surfaces, linking biogenesis to functional diversity, highlights therapeutic diagnostic potential diseases such as cancer cardiovascular disorders. Additionally, we explore emerging applications EVs, including machine-learning-assisted analysis, chemical integration, cross-system combinations. also discusses some key challenges clinical translation EV-related technologies.
Язык: Английский
Процитировано
1
Osteoarthritis and Cartilage, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
1
International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 2575 - 2592
Опубликована: Март 1, 2025
Small-interfering RNA (siRNA) therapy holds significant potential for treating cardiac injury; however, its clinical application is constrained by poor blood stability and insufficient cellular uptake. Extracellular vesicles (EVs) have emerged as an effective delivery system siRNA in vivo; but their lack of specific cell or tissue-targeting ability remains a major challenge. Thus, we aimed to develop EV-based capable targeted therapeutic injured tissue repair. To identify fibroblast activation protein (FAP) target tissue, analyzed tissues from patients with heart failure angiotensin II (Ang II)-treated mice. Injured EVs were developed embedding cholesterol-conjugated FAP aptamer, which specifically targets FAP, onto human serum-derived (hEV). Our findings revealed that upregulated after injury, highlighting tissues. We successfully aptamer-functionalized hEV (hEV@FAP) confirmed typical EV characteristics, including morphology, size distribution, zeta potential, marker expression. In addition, hEV@FAP demonstrated high targeting selectivity FAP-positive regions both vitro vivo. treat loaded TGFβ1 (siTGFβ1), identified molecular Ang II-treated mice, intravenous administration hEV@FAP-siTGFβ1 effectively reduced II-induced expression tissues, attributed the protective capabilities hEV@FAP. Consequently, significantly improved function, myocardial fibrosis, decreased cardiomyocyte cross-sectional area (P < 0.05) without inducing systemic toxicity. represents novel approach providing promising nanomedicine
Язык: Английский
Процитировано
0
Bioactive Materials, Год журнала: 2025, Номер 49, С. 218 - 254
Опубликована: Март 10, 2025
Язык: Английский
Процитировано
0
Frontiers in Pharmacology, Год журнала: 2025, Номер 16
Опубликована: Март 13, 2025
Background With the rapid development of extracellular vesicles (EVs) in regenerative medicine research, they have become a promising new direction mechanistic, diagnosis and treatment studies degenerative musculoskeletal diseases (DMDs), has attracted increasing attention. However, there is currently lack comprehensive objective summary analysis to help researchers quickly conveniently understand trajectory future trends this field. Method This study collected articles reviews published from 2006 2024 on EVs DMDs Web Science database. Bibliometric visual was conducted using several tools, including Microsoft Excel Office, VOSviewer, CiteSpace, Pajek, R packages. Results 1,182 publications were included 2024. Notably, increase number starting 2016, suggesting that field remains developmental stage. Co-authorship revealed China ranked first terms publications, whereas United States led citations. The journal with highest International Journal Molecular Sciences (INT J MOL SCI). most prolific authors Ragni, E 23 while cited author Toh, WS. Additionally, nine top 10 institutions China, Shanghai Jiao Tong University leading publications. article “MSC exosomes mediate cartilage repair by enhancing proliferation, attenuating apoptosis modulating immune reactivity”, authored Zhang, S, BIOMATERIALS 2018. Conclusion study, through bibliometric analysis, clearly illustrates collaborative relationships among countries, authors, institutions, journals, providing valuable insights for seeking academic collaboration opportunities. Moreover, keywords citations allows better key research hotspots frontiers field, points toward directions research. growing interest EV over recent years indicates attention dynamic progression
Язык: Английский
Процитировано
0
Environmental Pollution, Год журнала: 2025, Номер 372, С. 126064 - 126064
Опубликована: Март 15, 2025
Язык: Английский
Процитировано
0
Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 161653 - 161653
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0