Irradiation with cold atmospheric direct plasma: an innovative approach to treating murine cutaneous wounds DOI Creative Commons
Baida M. Ahmed, Sahar A. H. Al-Sharqi, Madyan A. Khalaf

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 11, 2024

Abstract Cold atmospheric direct plasma (CADP), an ionized gas at ambient temperature, represents a promising approach to enhancing tissue regeneration. A laboratory-based study was conducted investigate the effects of medical CADP on reparative potential full-thickness acute skin wounds in murine models. For vivo investigations, two dermal injuries were induced each subject, with diameter approximately 8 mm (n = 20). We employed floating electrode within system that generates pressure air plasma, characterized by temperature ranging from 36 38°C. The received three treatments, administered twice daily for irradiation durations 5, 15, and 25 seconds. These subsequently evaluated intervals 2, 4, 6, 8, 11 days post-wounding through histological examination gene expression analysis. On eleventh day, wound healing rates recorded 34.80% control group, while plasma-treated groups achieved 56.62%, 84.97%, 97.82%, respectively. Histological revealed plasma-treatment promotes development epidermal keratin granular strata, along formation hair follicles sebaceous glands. Gene analysis indicates increased levels growth factors decrease white blood cell counts. therapeutic intervention has significantly enhanced efficacy dermatological lesions without any noticeable adverse or simultaneous activation pro-inflammatory signaling pathways. findings underscore benefits employing applications management clinical settings.

Язык: Английский

N4-acetylcytidine modification of LncRNA GFOD1-AS1 promotes high glucose-induced dysfunction in human dermal microvascular endothelial cells through stabilization of DNMT1 protein DOI Creative Commons
Jingjing Yuan, Lusha Li,

Yang Lv

и другие.

Functional & Integrative Genomics, Год журнала: 2025, Номер 25(1)

Опубликована: Май 24, 2025

Emerging evidence supports that angiogenesis is essential for the wound healing of diabetic foot ulcer (DFU), and high glucose (HG)-induced dysfunction human dermal microvascular endothelial cells a key factor hinders angiogenesis. However, underlying mechanisms by which HG leads to has not been fully elucidated. In present investigation, we discovered significant upregulation long non-coding RNA GFOD1-AS1(GFOD1-AS1) in margin samples patients with DFU HG-induced model cells, attributing its dysregulation stabilizing effect NAT10-mediated ac4C modification, as corroborated an integrated approach data mining experimental validation. Subsequently, series vitro functional analyses showed ectopic expression GFOD1-AS1 promoted impaired function cells. contrast, knockdown significantly alleviated impairment indicated enhanced cell proliferation, migration, tube formation. Mechanistically, directly interacts DNA methyltransferase DNMT1 block ubiquitin-proteasome degradation, thereby enhancing protein stability DNMT1.This elevates expression, ultimately inducing summary, our results reveal serves potential therapeutic target DFU, highlight critical role NAT10/GFOD1-AS1/DNMT1 axis DFU.

Язык: Английский

Процитировано

0
Irradiation with cold atmospheric direct plasma: an innovative approach to treating murine cutaneous wounds DOI Creative Commons
Baida M. Ahmed, Sahar A. H. Al-Sharqi, Madyan A. Khalaf

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 11, 2024

Abstract Cold atmospheric direct plasma (CADP), an ionized gas at ambient temperature, represents a promising approach to enhancing tissue regeneration. A laboratory-based study was conducted investigate the effects of medical CADP on reparative potential full-thickness acute skin wounds in murine models. For vivo investigations, two dermal injuries were induced each subject, with diameter approximately 8 mm (n = 20). We employed floating electrode within system that generates pressure air plasma, characterized by temperature ranging from 36 38°C. The received three treatments, administered twice daily for irradiation durations 5, 15, and 25 seconds. These subsequently evaluated intervals 2, 4, 6, 8, 11 days post-wounding through histological examination gene expression analysis. On eleventh day, wound healing rates recorded 34.80% control group, while plasma-treated groups achieved 56.62%, 84.97%, 97.82%, respectively. Histological revealed plasma-treatment promotes development epidermal keratin granular strata, along formation hair follicles sebaceous glands. Gene analysis indicates increased levels growth factors decrease white blood cell counts. therapeutic intervention has significantly enhanced efficacy dermatological lesions without any noticeable adverse or simultaneous activation pro-inflammatory signaling pathways. findings underscore benefits employing applications management clinical settings.

Язык: Английский

Процитировано

0