Evaluation of betanin‐loaded liposomal nanocarriers in AlCl3‐induced Alzheimer's disease in rats: Impact on cognitive function, neurodegeneration, and TREM2/ADAM10 pathways DOI
Rania M. Salama, Rana Yehia, Noura F. Elmongy

и другие.

Archiv der Pharmazie, Год журнала: 2024, Номер unknown

Опубликована: Дек. 2, 2024

Abstract Betanin (BET) has been studied for its therapeutic benefits in various diseases, but low bioavailability and uncertain brain penetration limit efficacy. Accordingly, this study aimed to explore BET‐loaded liposomal nanocarriers (LPN) as a novel treatment Alzheimer's disease (AD), focusing on the triggering receptor expressed myeloid cells 2 (TREM2)/DNAX‐activating protein of 12 kDa (DAP12) extracellular signal‐regulated kinase 1/2 (ERK1/2) pathways implicated AD. In an AlCl 3 ‐induced AD rat model, 48 male Wistar rats were divided into four groups: control, (50 mg/kg, intraperitoneal), +BET (100 per os), LPN (25 intranasally), with treatments administered 28 days. Morris water maze test histopathological examination showed that BET LPN‐treated had improved spatial learning memory less hippocampal cortical degeneration compared oral groups. Mechanistically, corrected biomarkers, increased miR‐132 ADAM10 expression, reduced oxidative stress, inflammation, apoptosis. Additionally, suppressed expression TREM2, DAP12, ERK1/2, mitogen‐activated (MEK1/2), showing greater improvement than BET. These findings suggest enhances cognitive function neuroprotection by modulating inhibiting ERK1/2 TREM2/DAP12 pathways, providing more effective traditional administration.

Язык: Английский

Optimized Hesperidin-Loaded Lipid Nanoparticles with Tea Tree Oil for Enhanced Wound Healing: Formulation, Characterization, and Evaluation DOI Creative Commons
Borros Arneth, Rehab Abdelmonem, Mohamed A. El-Nabarawi

и другие.

Pharmaceuticals, Год журнала: 2025, Номер 18(3), С. 290 - 290

Опубликована: Фев. 20, 2025

Objectives: This study aimed to develop hesperidin solid lipid nanoparticles (HESP-SLNs) enhance their stability, solubility, and sustained release for wound healing; further enhancement was achieved through prepared nanostructured carriers (HESP-NLCs) using Tea Tree Oil (TTO) explore synergistic efficacy. Methods: A factorial design of 24 trials established evaluate the influence type (X1), conc (%) (X2), surfactant (X3), sonication amplitude (X4) HESP-SLNs on particle size (nm) (Y1), polydispersibility index (Y2), zeta potential (Y3), encapsulation efficiency (Y4). The optimized formula selected utilizing Design Expert® software version 13, which additionally enhanced by preparing TTO-loaded HESP-NLCs. In vitro release, Raman spectroscopy, transmission electron microscopy were carried out both nanoparticles. Cytotoxicity, in vivo wound-healing assessments, skin irritancy tests performed performance TTO-incorporated HESP-NLCs compared HESP-SLNs. Results: demonstrated PS (280 ± 1.35 nm), ZP (−39.4 0.92 mV), PDI (0.239 0.012), EE% (88.2 2.09%). NLCs Q6% (95.14%) vs. (79.69%), SLNs showed superior antimicrobial Both exhibited spherical morphology compatibility between HESP excipients. highest closure percentage, supported histological analysis inflammatory biomarker outcomes. Cytotoxicity evaluation 87% cell viability untreated HSF cells, test confirmed safety NLCs. Conclusions: are promising candidates exhibiting capabilities, making them a therapeutic option cutaneous management.

Язык: Английский

Процитировано

2

Formulation, development and in vivo characterization of selegiline hydrochloride nanostructured lipid nanocarrier loaded microneedle array patch for depression DOI
Anuradha Patil, Amarjitsing Rajput,

Praveen Subbappa

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер 671, С. 125257 - 125257

Опубликована: Янв. 22, 2025

Язык: Английский

Процитировано

1

Formulation Design and Evaluation of Ginsenoside Compound K Nanostructured Lipid Carriers Based on Molecular Dynamics Simulations DOI
Wei Shen,

Shili Pan,

Jingying Li

и другие.

Journal of Pharmaceutical Innovation, Год журнала: 2024, Номер 19(6)

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

1

Evaluation of betanin‐loaded liposomal nanocarriers in AlCl3‐induced Alzheimer's disease in rats: Impact on cognitive function, neurodegeneration, and TREM2/ADAM10 pathways DOI
Rania M. Salama, Rana Yehia, Noura F. Elmongy

и другие.

Archiv der Pharmazie, Год журнала: 2024, Номер unknown

Опубликована: Дек. 2, 2024

Abstract Betanin (BET) has been studied for its therapeutic benefits in various diseases, but low bioavailability and uncertain brain penetration limit efficacy. Accordingly, this study aimed to explore BET‐loaded liposomal nanocarriers (LPN) as a novel treatment Alzheimer's disease (AD), focusing on the triggering receptor expressed myeloid cells 2 (TREM2)/DNAX‐activating protein of 12 kDa (DAP12) extracellular signal‐regulated kinase 1/2 (ERK1/2) pathways implicated AD. In an AlCl 3 ‐induced AD rat model, 48 male Wistar rats were divided into four groups: control, (50 mg/kg, intraperitoneal), +BET (100 per os), LPN (25 intranasally), with treatments administered 28 days. Morris water maze test histopathological examination showed that BET LPN‐treated had improved spatial learning memory less hippocampal cortical degeneration compared oral groups. Mechanistically, corrected biomarkers, increased miR‐132 ADAM10 expression, reduced oxidative stress, inflammation, apoptosis. Additionally, suppressed expression TREM2, DAP12, ERK1/2, mitogen‐activated (MEK1/2), showing greater improvement than BET. These findings suggest enhances cognitive function neuroprotection by modulating inhibiting ERK1/2 TREM2/DAP12 pathways, providing more effective traditional administration.

Язык: Английский

Процитировано

0