Wheat germ agglutinin-nanoparticles encapsulating itacitinib target and suppress pro-inflammatory slan+ monocytes DOI
Karen Valdés Álvarez, Cristian Anacona,

Esneyder Ruiz Agudelo

и другие.

Nanomedicine, Год журнала: 2025, Номер 20(10), С. 1113 - 1126

Опубликована: Апрель 28, 2025

6-sulfoLacNAc (slan)+ monocytes, a non-classical monocyte subset, play pro-inflammatory role in autoimmune diseases like systemic lupus erythematosus (SLE). This study evaluates the therapeutic potential of itacitinib (ITA) encapsulated wheat germ agglutinin-functionalized nanoparticles (WGA/F127/PNPs) to target and inhibit JAK-STAT pathway slan+ monocytes. We prepared ITA-loaded WGA/F127/PNPs analyzed their binding internalization various leukocyte subsets using flow cytometry, focusing on slan- Further, peripheral blood samples from healthy controls (n = 37) SLE patients 50) were used assess phenotypes. Co-cultures monocytes stimulated with LPS revealed that significantly increased HLA-DR expression. Results showed reduced compared (p < 0.001) effectively internalized WGA/F127/PNPs, unlike cells. decreased HLA-DR, CD69, CD86 expression, STAT1 phosphorylation, cytokine production IFN-γ-stimulated Findings support as promising drug delivery system for targeting providing new SLE. suppress presenting promising, cell-specific approach managing related disorders.

Язык: Английский

Wheat germ agglutinin-nanoparticles encapsulating itacitinib target and suppress pro-inflammatory slan+ monocytes DOI
Karen Valdés Álvarez, Cristian Anacona,

Esneyder Ruiz Agudelo

и другие.

Nanomedicine, Год журнала: 2025, Номер 20(10), С. 1113 - 1126

Опубликована: Апрель 28, 2025

6-sulfoLacNAc (slan)+ monocytes, a non-classical monocyte subset, play pro-inflammatory role in autoimmune diseases like systemic lupus erythematosus (SLE). This study evaluates the therapeutic potential of itacitinib (ITA) encapsulated wheat germ agglutinin-functionalized nanoparticles (WGA/F127/PNPs) to target and inhibit JAK-STAT pathway slan+ monocytes. We prepared ITA-loaded WGA/F127/PNPs analyzed their binding internalization various leukocyte subsets using flow cytometry, focusing on slan- Further, peripheral blood samples from healthy controls (n = 37) SLE patients 50) were used assess phenotypes. Co-cultures monocytes stimulated with LPS revealed that significantly increased HLA-DR expression. Results showed reduced compared (p < 0.001) effectively internalized WGA/F127/PNPs, unlike cells. decreased HLA-DR, CD69, CD86 expression, STAT1 phosphorylation, cytokine production IFN-γ-stimulated Findings support as promising drug delivery system for targeting providing new SLE. suppress presenting promising, cell-specific approach managing related disorders.

Язык: Английский

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