Lipidized LL37-loaded PLGA nanocarriers: Bioengineered peptide delivery systems for enhanced wound healing DOI Creative Commons

Chiara De Soricellis,

Chloé Laigle,

Lucio Spinelli

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125668 - 125668

Опубликована: Апрель 1, 2025

Antimicrobial peptides (AMPs) such as LL37 offer a promising alternative to conventional antibiotics in treating chronic and multidrug-resistant wound infections. However, their clinical translation is limited by rapid degradation cytotoxicity at high concentrations. This study investigates the encapsulation of palmitoylated FDA-approved poly(lactic-co-glycolic acid) (PLGA) nanoparticles using two fabrication techniques, nanoprecipitation microfluidics, enhance stability controlled peptide release. Microfluidic-generated demonstrated superior size uniformity, smaller hydrodynamic (102.3 ± 2.0 nm vs 189.3 3.4 nm), improved stability, prolonged LL37(P) release compared obtained via bulk method. LL37-encapsulated release, enhanced keratinocyte uptake, significant fibroblast-mediated closure acceleration. Proteomic analysis nanoparticle-protein corona revealed enrichment proteins involved coagulation, inflammation modulation, extracellular matrix remodelling, suggesting an active role modulating healing microenvironment. These findings highlight PLGA-based loaded nanocarriers biopolymer platform for AMP delivery applications viable therapeutic strategy regenerative medicine infection control.

Язык: Английский

Lipidized LL37-loaded PLGA nanocarriers: Bioengineered peptide delivery systems for enhanced wound healing DOI Creative Commons

Chiara De Soricellis,

Chloé Laigle,

Lucio Spinelli

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125668 - 125668

Опубликована: Апрель 1, 2025

Antimicrobial peptides (AMPs) such as LL37 offer a promising alternative to conventional antibiotics in treating chronic and multidrug-resistant wound infections. However, their clinical translation is limited by rapid degradation cytotoxicity at high concentrations. This study investigates the encapsulation of palmitoylated FDA-approved poly(lactic-co-glycolic acid) (PLGA) nanoparticles using two fabrication techniques, nanoprecipitation microfluidics, enhance stability controlled peptide release. Microfluidic-generated demonstrated superior size uniformity, smaller hydrodynamic (102.3 ± 2.0 nm vs 189.3 3.4 nm), improved stability, prolonged LL37(P) release compared obtained via bulk method. LL37-encapsulated release, enhanced keratinocyte uptake, significant fibroblast-mediated closure acceleration. Proteomic analysis nanoparticle-protein corona revealed enrichment proteins involved coagulation, inflammation modulation, extracellular matrix remodelling, suggesting an active role modulating healing microenvironment. These findings highlight PLGA-based loaded nanocarriers biopolymer platform for AMP delivery applications viable therapeutic strategy regenerative medicine infection control.

Язык: Английский

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