medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Сен. 8, 2023
Abstract
The
spatial
and
temporal
changes
of
the
COVID-19
pandemic
have
been
monitored
with
wastewater-based
surveillance,
which
many
countries
applied
to
their
national
public
health
monitoring
measures.
most
commonly
used
methods
for
detection
SARS-CoV-2
in
wastewater
are
RT-qPCR
RT-ddPCR.
Previous
comparisons
two
produced
conflicting
results;
some
found
RT-ddPCR
be
more
sensitive,
one
others
them
equal
sensitivity.
This
research
was
conducted
further
study
these
as
well
different
RNA
extraction
methodologies
gene
assays
wastewater.
We
compared
kits
based
on
sensitivity,
variability,
correlation
copy
numbers
incidence
COVID-19.
Our
results
indicate
that
sensitive
low-variance
method
detect
However,
we
obtained
best
between
number
using
(CC
=
0.697,
p
<
0.001).
a
significant
difference
sensitivity
kits,
having
significantly
lower
limit
higher
percentage
positive
samples
than
other.
Furthermore,
CDC
N1
primers
probe
were
both
while
there
no
tested
targets
For
RT-qPCR,
use
affected
result.
All
showed
trend
In
addition,
an
isothermal
amplification
It
proved
viable
option
if
expected
quickly,
resources
limited,
presence–absence
information
is
sufficient.
Highlights
Using
produces
differing
results.
copies
correlate
reported
Finland.
repeatable
wastewater,
whereas
had
RT-SIBA
low-resource
settings.
high
result
variability
when
amount
low.
Graphical
abstract
Created
BioRender.com
Heliyon,
Год журнала:
2024,
Номер
10(13), С. e33873 - e33873
Опубликована: Июнь 29, 2024
A
focus
on
water
quality
has
intensified
globally,
considering
its
critical
role
in
sustaining
life
and
ecosystems.
Wastewater,
reflecting
societal
development,
profoundly
impacts
public
health.
Wastewater-based
epidemiology
(WBE)
emerged
as
a
surveillance
tool
for
detecting
outbreaks
early,
monitoring
infectious
disease
trends,
providing
real-time
insights,
particularly
vulnerable
communities.
WBE
aids
tracking
pathogens,
including
viruses,
sewage,
offering
comprehensive
understanding
of
community
health
lifestyle
habits.
With
the
rise
global
COVID-19
cases,
gained
prominence,
aiding
SARS-CoV-2
levels
worldwide.
Despite
advancements
treatment,
poorly
treated
wastewater
discharge
remains
threat,
amplifying
spread
water-,
sanitation-,
hygiene
(WaSH)-related
diseases.
WBE,
serving
complementary
surveillance,
is
pivotal
community-level
viral
infections.
However,
there
untapped
potential
to
expand
surveillance.
This
review
emphasizes
importance
link
between
health,
highlighting
need
further
integration
into
management.
Frontiers in Public Health,
Год журнала:
2023,
Номер
11
Опубликована: Март 21, 2023
Molecular
analysis
of
public
wastewater
has
great
potential
as
a
harbinger
for
community
health
and
threats.
Long-used
to
monitor
the
presence
enteric
viruses,
in
particular
polio,
recent
successes
reliable
lead
indicator
trends
SARS-CoV-2
levels
hospital
admissions
generated
optimism
emerging
evidence
that
similar
science
can
be
applied
other
pathogens
pandemic
(PPPs),
especially
respiratory
viruses
their
variants
concern
(VOC).
However,
there
are
substantial
challenges
associated
with
implementation
this
ideal,
namely
multiple
distinct
fields
inquiry
must
bridged
coordinated.
These
include
engineering,
molecular
sciences,
temporal-geospatial
analytics,
epidemiology
medical,
governmental
messaging,
all
which
present
own
caveats.
Here,
we
outline
framework
an
integrated,
state-wide,
end-to-end
human
pathogen
monitoring
program
using
track
viral
PPPs.
Water Research,
Год журнала:
2025,
Номер
274, С. 123114 - 123114
Опубликована: Янв. 7, 2025
The
post-pandemic
world
still
faces
ongoing
COVID-19
infections,
although
international
travel
has
returned
to
pre-pandemic
conditions.
Wastewater-based
epidemiology
(WBE)
is
considered
an
efficient
tool
for
the
population-wide
surveillance
of
infections
during
pandemic.
However,
performance
WBE
in
era
with
restrictions
lifted
remains
unknown.
Utilizing
weekly
county-level
wastewater
data
from
June
2021-November
2022
222
counties
49
states
(covering
104
million
people)
United
States
America,
we
retrospectively
evaluated
correlations
between
SARS-CoV-2
RNA
(C
Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi,
Год журнала:
2025,
Номер
15(1), С. 241 - 251
Опубликована: Янв. 29, 2025
Objective:
The
aim
of
this
study
is
to
evaluate
the
effectiveness
three
commercial
nucleic
acid
extraction
kits
(kit
A,
B
and
C)
in
isolating
SARS-CoV-2
viral
RNA
from
wastewater
samples.
Method:
In
study,
water
samples
were
collected
March
2021
treatment
plants
located
different
parts
Istanbul,
it
was
confirmed
that
they
negative
for
SARS-CoV-2.
Different
concentrations
virus,
previously
inactivated
at
BSL-3
laboratory
Pendik
Veterinary
Control
Institute,
added
quantification
performed
using
RT-qPCR
kit
specific
Results:
At
end
determined
C
yielded
highest
total
produced
more
consistent
results,
significantly
outperforming
other
two
terms
yield
purity.
Statistical
analysis
revealed
significant
differences
(p
<
0.05)
gene
copy
numbers
0.01)
between
kits,
demonstrated
superior
linearity
reproducibility.
Conclusion:
According
findings,
although
all
evaluated
are
suitable
detecting
samples,
provides
most
efficient
reliable
performance,
especially
high-throughput
studies.
Additionally,
highlights
importance
selecting
appropriate
methods
surveillance,
which
serves
as
an
early
warning
system
outbreaks
threaten
public
health.
