bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 22, 2023

Abstract Seagrass ecosystems provide crucial ecosystem services for coastal environments and were shown to reduce the abundance of pathogens linked infections in humans marine organisms. Among several potential drivers, seagrass phenolics released into seawater have been suggested play role pathogen suppression, but involvement microbiome such effect has not studied. Here we hypothesized that eelgrass Zostera marina , especially leaf epiphytes are at direct interface between host surrounding seawater, inhibit pathogenic microorganisms, hence, contribute their suppression. Using a culture-dependent approach, isolated 88 bacteria fungi associated with surfaces inner tissues leaves (healthy decaying) roots, plus 19 strains from sediment. We first assessed broad-spectrum antibiotic activity microbial extracts against large panel common aquatic, human (fecal) plant pathogens, finally mined metabolome most active extracts. The healthy epibiotic bacteria, particularly Streptomyces sp. strain 131, displayed potent superior some control drugs. Gram-negative abundant on surfaces, few endosphere-associated also showed remarkable antimicrobial activity. UPLC-MS/MS-based massive untargeted metabolomics analyses rich specialized metabolite repertoire low annotation rates, indicating presence many undescribed antimicrobials This study contributes our current understanding chemical ecology seagrasses, implying microbiome, epiphytes, reduction load seawater. Such is only beneficial health ocean, aquaculture sector, context climate change expected exacerbate all infectious diseases, may assist conservation management strategies.

Язык: Английский