ACS ES&T Engineering,
Год журнала:
2024,
Номер
4(9), С. 2177 - 2185
Опубликована: Авг. 5, 2024
This
study
tested
polyhydroxyalkanoate
(PHA)
production
by
usingPseudomonas
putida
KT2440
in
a
5
L
lab-scale
bioreactor,
employing
an
A
cell
dry
mass
concentration
of
8.69
±
0.45
g/L
was
achieved
with
volatile
fatty
acid
(VFA)
removal
efficiency
81.75
3.08%.
Remarkably,
this
the
highest
PHA
fraction
(34.81
1.67%).
Analysis
revealed
that
poly(3-hydroxybutyrate)
(PHB)
contributes
83.6
to
86.1%
total
followed
poly(3-hydroxyhexanoate)
(PHHx)
(6.6–8.6%)
and
poly(3-hydroxy-2-methylvalerate)
(PH2MV)
(4.9–8.3%).
Thermogravimetric
analysis
showed
polymer
started
degrading
at
temperature
150
°C
maximum
weight
loss
occurring
approximately
270
°C.
Differential
scanning
calorimetry
two
peaks
corresponding
melting
polymer:
140.6,
154.87
suggests
resulting
blend
different
copolymers.
outcome
is
poised
advance
development
unified
methods
within
circular
bioeconomy,
enhancing
both
economic
environmental
advantages.
