Genome editing in ubiquitous freshwater Actinobacteria DOI Creative Commons
Nachiketa Bairagi, Jessica L. Keffer,

Jordan C. Heydt

и другие.

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(11)

Опубликована: Окт. 16, 2024

ABSTRACT Development of genome-editing tools in diverse microbial species is an important step both understanding the roles those microbes different environments, and engineering for a variety applications. Freshwater-specific clades Actinobacteria are ubiquitous abundant surface freshwaters worldwide. Here, we show that Rhodoluna lacicola Aurantimicrobium photophilum , which represent widespread freshwater Actinobacteria, naturally transformable. We also gene inactivation via double homologous recombination replacement target with antibiotic selection markers can be used strains, making them convenient broadly accessible model organisms systems. further predicted phytoene synthase only synthase, its prevents synthesis all pigments. The developed here enable targeted modification genomes some most communities. These will hypothesis testing about genetics (eco)physiology broaden available systems IMPORTANCE To advance bioproduction or bioremediation large, unsupervised environmental such as ponds, wastewater lagoons, groundwater systems, it necessary to develop genetically amenable organisms. Although already modify few key species, more taxa, natural phenotypes, us engineer multispecies consortia even complex Developing genetic modifying bacteria particularly important, wastewater, production ponds raceways, contaminated water where communities deployed do work, outputs could potentially enhanced by modifications. demonstrate common genome editing inactivate specific genes two representatives very widespread, environmentally relevant group Actinobacteria. found almost tested they co-occur primary producers, these thus on way environments.

Язык: Английский

Unlocking the adaptation mechanisms of the oleaginous microalga Scenedesmus sp. BHU1 under elevated salt stress: a physiochemical, lipidomics and transcriptomics approach DOI Creative Commons
Rahul Prasad Singh, Priya Yadav,

Himani Sharma

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 18, 2024

Microalgae are vital for their photosynthetic abilities, contributing significantly to global oxygen production, serving as a key trophic level in aquatic ecosystems, aiding biofuel assisting wastewater treatment, and facilitating the synthesis of valuable biochemicals. Despite these advantages, microalgae sensitive salt stress, which alters physiochemical metabolic status, ultimately reducing microalgal growth. This sensitivity highlights importance understanding impact elevated content on physiochemical, metabolic, transcriptomic profiling Scenedesmus sp., areas that not yet fully understood. Our findings indicate stress decreases efficiency increases non-regulated photochemical quenching photosystem II (PSII). Moreover, PSII-driven linear electron flow (LEF) decreased, whereas I (PSI)-driven cyclic (CEF) increased salt-stressed cells. To better understand from PSII PSI under we analyzed excitation energy flux per reaction center (RC), cross-section (CS), ratios, potential index PSII. Additionally, cytometry graphs depict viability assay sp. BHU1. observations further revealed an increase biochemical attributes, such biomarkers, osmoprotectants, enzymatic antioxidants, help scavenge reactive species (ROS) stress. Intracellular cations (Na + Ca 2+ ) were increased, while K levels indicating mechanisms cellular homeostasis UHPLC-HRMS-based lipidome analysis confirmed increasing induces hyperaccumulation several fatty acids involved adaptation. transcriptome upregulation genes associated with PSI, glycolysis, starch metabolism, sucrose lipid accumulation In contrast, related C3 carbon fixation downregulated mitigate adverse effects

Язык: Английский

Процитировано

6
Uso de microalgas en la industria química de Colombia: una revisión DOI Creative Commons
Johan Manuel Cifuentes

Revista Mutis, Год журнала: 2025, Номер 15(1), С. 1 - 25

Опубликована: Апрель 10, 2025

Las microalgas, microorganismos fotosintéticos fundamentales en los ecosistemas acuáticos, destacan por su capacidad de realizar fotosíntesis oxigénica, equilibrar el oxígeno y promover flujo energético. Su rápido crecimiento, alta adaptabilidad, elevado contenido lipídico fijar carbono han despertado un interés creciente las últimas décadas, con la identificación cerca 100,000 especies distribuidas globalmente. Históricamente sido utilizadas como alimento aplicaciones terapéuticas desde hace más 2000 años. Actualmente se reconocen fuentes compuestos bioactivos, incluyendo ácidos grasos poliinsaturados, carotenoides vitaminas, amplias industrias alimenticia, cosmética, energética salud. Este estudio revisó 53 artículos indexados Scopus Publindex publicados entre 2012 2024, clasificando microalgas industria química colombiana áreas farmacéutica, biofertilizantes, biorremediación, nutrición biocombustibles. Se identificaron clases Chlorophyceae, Trebouxiophyceae Cyanophyceae estudiadas, especialmente géneros Scenedesmus sp., Chlorella sp. Arthrospira principales concentran biorremediación aguas producción biocombustibles, destacando departamentos Antioquia Norte Santander relevantes. Finalmente, subraya necesidad profundizar estudios a nivel especie avanzar investigaciones bajo condiciones campo. También resalta importancia fortalecer farmacéuticas biomédicas, biofertilizantes humana animal, para maximizar potencial contexto biodiversidad colombiana.

Процитировано

0
Marine Microalgae–Microorganism Co-Cultures: An Insight into Nannochloropsis sp. Use and Biotechnological Applications DOI Creative Commons
Marta Vala Esteves, Diana M. C. Marques,

Joana Dias de Almeida

и другие.

Foods, Год журнала: 2025, Номер 14(9), С. 1522 - 1522

Опубликована: Апрель 26, 2025

The increasing demand for sustainable, economical, and environmentally friendly solutions has positioned microalgae as promising candidates in biotechnology, particularly food, feed, nutraceutical, pharmaceutical, biofuel, bioremediation applications. This review explores the role of Nannochloropsis genus other marine oleaginous co-cultivation systems, highlighting their mechanisms interaction with various microorganisms potential biotechnological purposes. Case studies sp. co-cultures microalgae, bacteria, fungi are presented. different types associations described alternative strategies to enhance biomass productivity, lipid accumulation, nutrient recycling. A key focus this is it part list be approved consumption European Union, discussing rich nutritional value, safety, regulatory status. Additionally, protein sector explored, particular emphasis on integration cultivated meat products suppliers metabolic partners animal cells. Despite potential, several challenges, such scale-up, contamination risk, strain selection, remain obstacles widespread adoption microalgal biotechnology. Future research should optimizing microalgae-based food applications, addressing safety concerns, further investigating into functional foods cellular agriculture products.

Язык: Английский

Процитировано

0
Genome editing in ubiquitous freshwater Actinobacteria DOI Creative Commons
Nachiketa Bairagi, Jessica L. Keffer,

Jordan C. Heydt

и другие.

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(11)

Опубликована: Окт. 16, 2024

ABSTRACT Development of genome-editing tools in diverse microbial species is an important step both understanding the roles those microbes different environments, and engineering for a variety applications. Freshwater-specific clades Actinobacteria are ubiquitous abundant surface freshwaters worldwide. Here, we show that Rhodoluna lacicola Aurantimicrobium photophilum , which represent widespread freshwater Actinobacteria, naturally transformable. We also gene inactivation via double homologous recombination replacement target with antibiotic selection markers can be used strains, making them convenient broadly accessible model organisms systems. further predicted phytoene synthase only synthase, its prevents synthesis all pigments. The developed here enable targeted modification genomes some most communities. These will hypothesis testing about genetics (eco)physiology broaden available systems IMPORTANCE To advance bioproduction or bioremediation large, unsupervised environmental such as ponds, wastewater lagoons, groundwater systems, it necessary to develop genetically amenable organisms. Although already modify few key species, more taxa, natural phenotypes, us engineer multispecies consortia even complex Developing genetic modifying bacteria particularly important, wastewater, production ponds raceways, contaminated water where communities deployed do work, outputs could potentially enhanced by modifications. demonstrate common genome editing inactivate specific genes two representatives very widespread, environmentally relevant group Actinobacteria. found almost tested they co-occur primary producers, these thus on way environments.

Язык: Английский

Процитировано

0