Unlocking the adaptation mechanisms of the oleaginous microalga Scenedesmus sp. BHU1 under elevated salt stress: a physiochemical, lipidomics and transcriptomics approach
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 18, 2024
Microalgae
are
vital
for
their
photosynthetic
abilities,
contributing
significantly
to
global
oxygen
production,
serving
as
a
key
trophic
level
in
aquatic
ecosystems,
aiding
biofuel
assisting
wastewater
treatment,
and
facilitating
the
synthesis
of
valuable
biochemicals.
Despite
these
advantages,
microalgae
sensitive
salt
stress,
which
alters
physiochemical
metabolic
status,
ultimately
reducing
microalgal
growth.
This
sensitivity
highlights
importance
understanding
impact
elevated
content
on
physiochemical,
metabolic,
transcriptomic
profiling
Scenedesmus
sp.,
areas
that
not
yet
fully
understood.
Our
findings
indicate
stress
decreases
efficiency
increases
non-regulated
photochemical
quenching
photosystem
II
(PSII).
Moreover,
PSII-driven
linear
electron
flow
(LEF)
decreased,
whereas
I
(PSI)-driven
cyclic
(CEF)
increased
salt-stressed
cells.
To
better
understand
from
PSII
PSI
under
we
analyzed
excitation
energy
flux
per
reaction
center
(RC),
cross-section
(CS),
ratios,
potential
index
PSII.
Additionally,
cytometry
graphs
depict
viability
assay
sp.
BHU1.
observations
further
revealed
an
increase
biochemical
attributes,
such
biomarkers,
osmoprotectants,
enzymatic
antioxidants,
help
scavenge
reactive
species
(ROS)
stress.
Intracellular
cations
(Na
+
Ca
2+
)
were
increased,
while
K
levels
indicating
mechanisms
cellular
homeostasis
UHPLC-HRMS-based
lipidome
analysis
confirmed
increasing
induces
hyperaccumulation
several
fatty
acids
involved
adaptation.
transcriptome
upregulation
genes
associated
with
PSI,
glycolysis,
starch
metabolism,
sucrose
lipid
accumulation
In
contrast,
related
C3
carbon
fixation
downregulated
mitigate
adverse
effects
Язык: Английский
Uso de microalgas en la industria química de Colombia: una revisión
Revista Mutis,
Год журнала:
2025,
Номер
15(1), С. 1 - 25
Опубликована: Апрель 10, 2025
Las
microalgas,
microorganismos
fotosintéticos
fundamentales
en
los
ecosistemas
acuáticos,
destacan
por
su
capacidad
de
realizar
fotosíntesis
oxigénica,
equilibrar
el
oxígeno
y
promover
flujo
energético.
Su
rápido
crecimiento,
alta
adaptabilidad,
elevado
contenido
lipídico
fijar
carbono
han
despertado
un
interés
creciente
las
últimas
décadas,
con
la
identificación
cerca
100,000
especies
distribuidas
globalmente.
Históricamente
sido
utilizadas
como
alimento
aplicaciones
terapéuticas
desde
hace
más
2000
años.
Actualmente
se
reconocen
fuentes
compuestos
bioactivos,
incluyendo
ácidos
grasos
poliinsaturados,
carotenoides
vitaminas,
amplias
industrias
alimenticia,
cosmética,
energética
salud.
Este
estudio
revisó
53
artículos
indexados
Scopus
Publindex
publicados
entre
2012
2024,
clasificando
microalgas
industria
química
colombiana
áreas
farmacéutica,
biofertilizantes,
biorremediación,
nutrición
biocombustibles.
Se
identificaron
clases
Chlorophyceae,
Trebouxiophyceae
Cyanophyceae
estudiadas,
especialmente
géneros
Scenedesmus
sp.,
Chlorella
sp.
Arthrospira
principales
concentran
biorremediación
aguas
producción
biocombustibles,
destacando
departamentos
Antioquia
Norte
Santander
relevantes.
Finalmente,
subraya
necesidad
profundizar
estudios
a
nivel
especie
avanzar
investigaciones
bajo
condiciones
campo.
También
resalta
importancia
fortalecer
farmacéuticas
biomédicas,
biofertilizantes
humana
animal,
para
maximizar
potencial
contexto
biodiversidad
colombiana.
Marine Microalgae–Microorganism Co-Cultures: An Insight into Nannochloropsis sp. Use and Biotechnological Applications
Foods,
Год журнала:
2025,
Номер
14(9), С. 1522 - 1522
Опубликована: Апрель 26, 2025
The
increasing
demand
for
sustainable,
economical,
and
environmentally
friendly
solutions
has
positioned
microalgae
as
promising
candidates
in
biotechnology,
particularly
food,
feed,
nutraceutical,
pharmaceutical,
biofuel,
bioremediation
applications.
This
review
explores
the
role
of
Nannochloropsis
genus
other
marine
oleaginous
co-cultivation
systems,
highlighting
their
mechanisms
interaction
with
various
microorganisms
potential
biotechnological
purposes.
Case
studies
sp.
co-cultures
microalgae,
bacteria,
fungi
are
presented.
different
types
associations
described
alternative
strategies
to
enhance
biomass
productivity,
lipid
accumulation,
nutrient
recycling.
A
key
focus
this
is
it
part
list
be
approved
consumption
European
Union,
discussing
rich
nutritional
value,
safety,
regulatory
status.
Additionally,
protein
sector
explored,
particular
emphasis
on
integration
cultivated
meat
products
suppliers
metabolic
partners
animal
cells.
Despite
potential,
several
challenges,
such
scale-up,
contamination
risk,
strain
selection,
remain
obstacles
widespread
adoption
microalgal
biotechnology.
Future
research
should
optimizing
microalgae-based
food
applications,
addressing
safety
concerns,
further
investigating
into
functional
foods
cellular
agriculture
products.
Язык: Английский
Genome editing in ubiquitous freshwater Actinobacteria
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
90(11)
Опубликована: Окт. 16, 2024
ABSTRACT
Development
of
genome-editing
tools
in
diverse
microbial
species
is
an
important
step
both
understanding
the
roles
those
microbes
different
environments,
and
engineering
for
a
variety
applications.
Freshwater-specific
clades
Actinobacteria
are
ubiquitous
abundant
surface
freshwaters
worldwide.
Here,
we
show
that
Rhodoluna
lacicola
Aurantimicrobium
photophilum
,
which
represent
widespread
freshwater
Actinobacteria,
naturally
transformable.
We
also
gene
inactivation
via
double
homologous
recombination
replacement
target
with
antibiotic
selection
markers
can
be
used
strains,
making
them
convenient
broadly
accessible
model
organisms
systems.
further
predicted
phytoene
synthase
only
synthase,
its
prevents
synthesis
all
pigments.
The
developed
here
enable
targeted
modification
genomes
some
most
communities.
These
will
hypothesis
testing
about
genetics
(eco)physiology
broaden
available
systems
IMPORTANCE
To
advance
bioproduction
or
bioremediation
large,
unsupervised
environmental
such
as
ponds,
wastewater
lagoons,
groundwater
systems,
it
necessary
to
develop
genetically
amenable
organisms.
Although
already
modify
few
key
species,
more
taxa,
natural
phenotypes,
us
engineer
multispecies
consortia
even
complex
Developing
genetic
modifying
bacteria
particularly
important,
wastewater,
production
ponds
raceways,
contaminated
water
where
communities
deployed
do
work,
outputs
could
potentially
enhanced
by
modifications.
demonstrate
common
genome
editing
inactivate
specific
genes
two
representatives
very
widespread,
environmentally
relevant
group
Actinobacteria.
found
almost
tested
they
co-occur
primary
producers,
these
thus
on
way
environments.
Язык: Английский