Gene Expression Profiling Based on High-Throughput Sequencing Technology and Its Application in Disease Diagnosis
Journal of Prevention Diagnosis and Management of Human Diseases,
Год журнала:
2025,
Номер
51, С. 32 - 44
Опубликована: Янв. 13, 2025
Pulmonary
tuberculosis
tends
to
co-occur
with
other
diseases,
leading
the
emergence
of
drug-resistant
strains
Mycobacterium
tuberculosis,
which
complicates
identification
and
treatment
pulmonary
tuberculosis.
The
limitations
previous
methods
have
become
increasingly
evident.
Therefore,
based
on
high-throughput
sequencing
technology,
this
study
aims
screen
for
diagnostic
marker
genes
by
comparing
differences
in
PBMC
expression
profiles
between
patients
healthy
individuals.
During
research
process,
PBMCs
were
first
isolated
from
samples,
total
RNA
was
extracted,
cDNA
libraries
prepared
bridge
PCR
amplification.
Subsequently,
deep
performed
using
an
Illumina
Genome
Analyzer
IIx
sequencer
determine
read
counts
target
genes,
calibrate
sequence
tags
data,
differentially
expressed
PTB
HC
higher
levels,
make
diagnoses
condition.
Язык: Английский
Sustainable tourism for climate change and environmental sustainability in Tunisia: Evidence from a novel measure, nonlinear modeling, and wavelet coherence
Journal of Environmental Management,
Год журнала:
2025,
Номер
380, С. 124991 - 124991
Опубликована: Март 19, 2025
Язык: Английский
Niche Tourism
IGI Global eBooks,
Год журнала:
2025,
Номер
unknown, С. 147 - 170
Опубликована: Март 26, 2025
As
the
tourism
industry
takes
a
big
leap
and
continuously
flourishes,
concern
on
its
sustainability
is
significant
matter
for
all
stakeholders
of
industry.
Thus,
planning,
development
marketing
products
services
have
become
more
valuable
as
destinations
seen
contribution
in
national
particularly
economic,
social,
cultural
environmental
aspects.
This
chapter
presents
niche
tourism,
concept
principles,
evident
strategies
responses
to
overtourism
some
selected
areas,
considerations
high
value
low
volume
tourists
destinations.
Язык: Английский