Jujing
Zhuyu
decoction
(JZD)
is
a
traditional
Chinese
medicine
that
effectively
improves
sperm
motility.
However,
the
molecular
mechanism
of
JZD
on
asthenozoospermia
still
remains
unknown.
In
this
study,
we
investigated
effect
mitochondrial
apoptosis
pathway
in
an
rat
model.
Sixty
Sprague-Dawley
rats
were
randomly
divided
into
five
groups-control,
tripterygium
glycosides
(GTW)
model,
JZD-low
(JZD-L),
JZD-medium
(JZD-M),
and
JZD-high
(JZD-H)
groups
(n
=
12/group).
GTW
was
used
to
generate
The
JZD-L,
JZD-M,
JZD-H
administered
5,
10,
or
15
g
kg-1
day-1
granules
respectively,
for
4
weeks.
Testicular
tissue
morphology
examined
using
histological
staining,
while
count
determined
manual
computer-aided
semen
analyses.
Apoptosis
spermatogenic
cells
detected
with
TUNEL
assay,
expression
proteins
genes
related
western
blotting
quantitative
reverse
transcription-polymerase
chain
reaction
respectively.
Histomorphological
evaluation
revealed
superior
seminiferous
tubule
structure
arrangement
as
well
improved
cell
compared
those
model
group.
Moreover,
quality
apoptotic
index
significantly
Additionally,
mRNA
protein
abundance
Apaf-1,
Bax,
Cyto-c,
caspase-3
reduced,
Bcl-2
increased
all
reduces
rate
promotes
survival
by
regulating
pathway.
This
provides
experimental
support
treatment
JZD.
Current Pharmaceutical Design,
Год журнала:
2024,
Номер
30(34), С. 2718 - 2735
Опубликована: Июль 30, 2024
Background:
Oral
mucositis
is
the
most
common
and
troublesome
complication
for
cancer
patients
receiving
radiotherapy
or
chemotherapy.
Recent
research
has
shown
that
Lycium
barbarum,
an
important
economic
crop
widely
grown
in
China,
epithelial
protective
effects
several
other
organs.
However,
it
unknown
whether
not
barbarum
can
exert
a
beneficial
effect
on
oral
mucositis.
Network
pharmacology
been
suggested
to
be
applied
“multi-component-multi-target”
functional
food
studies.
The
purpose
of
this
study
evaluate
through
network
pharmacology,
molecular
docking
experimental
validation.
Aims:
To
explore
biological
mechanisms
treatment
combined
with
Methods:
Based
methods,
we
collected
active
components
related
targets
from
public
databases,
as
well
We
mapped
protein-
protein
interaction
(PPI)
networks,
performed
gene
ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment,
constructed
'components-disease-targets'
'components-
pathways-targets'
using
Cytoscape
further
analyse
intrinsic
against
affinity
stability
predictions
were
strategies,
experiments
conducted
demonstrate
possible
Results:
A
was
established
between
49
61
OM
targets.
main
compounds
quercetin,
beta-carotene,
palmatine,
cyanin.
predicted
core
IL-6,
RELA,
TP53,
TNF,
IL10,
CTNNB1,
AKT1,
CDKN1A,
HIF1A
MYC.
enrichment
analysis
therapeutic
mainly
regulation
inflammation,
apoptosis,
hypoxia
response
involvement
TNF
HIF
pathways.
Molecular
results
showed
key
bind
In
both
chemically
radiation-induced
models,
significantly
promoted
healing
reduced
inflammation.
verification
targeted
genes
(IL-6,
HIF1A,
MYC)
regulating
signaling
pathways,
which
validated
RT-qPCR,
immunofluorescence
staining
western
blotting
assays.
Conclusion:
conclusion,
present
systematically
demonstrated
could
promote
reduce
inflammatory
Translational Andrology and Urology,
Год журнала:
2024,
Номер
13(10), С. 2268 - 2279
Опубликована: Окт. 1, 2024
According
to
the
current
situation,
treatment
of
oligoasthenospermia
with
Western
medicine
is
still
full
challenges.
Qiang
Jing
decoction
(QJD)
used
for
treating
male
infertility
as
a
traditional
Chinese
(TCM)
prescription.
In
this
study.
we
will
try
uncover
molecular
mechanism
QJDs
in
based
on
proteomics
technology.
Chemistry & Biodiversity,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 23, 2024
Abstract
Wuzi
Yanzong
decoction
(WZYZD)
belongs
to
the
traditional
formula
for
treating
male
infertility
caused
by
oligoasthenozoospermia
(OLI).
This
research
aims
elucidate
therapeutic
substance
basis
and
potential
pharmacological
mechanisms
of
WZYZD
in
OLI.
A
total
52
chemical
ingredients
were
identified
from
WZYZD.
HE
TUNEL
staining
demonstrated
that
can
markedly
alleviate
Immunofluorescence
analysis
showed
significantly
increase
expression
levels
DNMT3
A,
PIWIL1,
SETDB1,
PRMT5.
Methyl
capture
sequencing
proved
upregulate
methylated
level
Spata
,
Bcl
Pik3
series
genes.
Network
pharmacology
ameliorate
OLI
through
BCL‐2
PI3
K‐AKT
signaling
pathways.
The
immunofluorescence
assay
SPATA18
aforementioned
results.
mechanism
mainly
involved
recruiting
methyltransferase
PRMT5,
SETDB1
increasing
methylation
degree
American Journal of Men s Health,
Год журнала:
2024,
Номер
18(6)
Опубликована: Ноя. 1, 2024
Guijiajiao-Lujiaojiao
(GL)
is
a
combination
of
Traditional
Chinese
Medicine
(TCM)
that
can
be
used
to
treat
oligoasthenozoospermia
(OAS).
However,
its
mechanistic
role
in
OAS
needs
better
understood
and
necessitates
more
studies.
This
study
was
planned
investigate
GL's
therapeutic
effects
the
tripterygium
wilfordii
polyglycoside
(GTW)-induced
rat
model.
In
total,
60
Sprague-Dawley
(SD)
rats
at
8
weeks
age
were
assigned
six
groups:
blank
(NC),
model
(GTW),
GL
low-dose
(GL-L,
0.3
g/kg/day),
medium-dose
(GL-M,
0.6
high-dose
(GL-H,
1.2
+
PI3K
inhibitor
LY294002
(GL-H
g/kg/day
LY
mg/kg/day)
groups.
The
characterized
after
examine
sperm
concentration
viability,
serum
hormone
levels,
testes
histopathology,
specific
protein
markers.
treatment
efficacy
evaluated
by
mRNA
expression
among
other
parameters.
Compared
with
GTW
group,
viability
spermatozoa
significantly
increased
intervention
(
Pharmaceutical Biology,
Год журнала:
2022,
Номер
60(1), С. 1533 - 1541
Опубликована: Авг. 9, 2022
Wuzi
Yanzong
Prescription
(WYP)
has
long
been
used
to
treat
male
infertility,
with
convincing
clinical
evidence.
However,
its
mechanism
of
action
is
not
clear.WYP
chemical
components
were
quantified
by
HPLC,
and
the
effect
on
oligoasthenozoospermia
rats
was
explored
based
UPLC-Q-TOF-MS
metabonomics
technology.The
solution
extracted
alcohol
water;
content
eight
in
extracting
determined
HPLC.
Twenty-four
Sprague-Dawley
randomly
divided
into
control
(CG),
model
(MG)
administration
(PG)
groups.
Oligoasthenozoospermia
induced
for
30
days
all,
but
CG
daily
oral
doses
20
mg/kg
Tripterysium
glycosides
(TG).
PG
given
WYP
(1.08
g/kg),
MG
received
same
volume
distilled
water,
all
administered
days.
After
last
administration,
serum
samples
collected
detected
UPLC-Q-TOF-MS.An
HPLC
method
simultaneous
determination
chlorogenic
acid
(0.081%),
ellagic
(0.021%),
hyperoside
(0.032%),
verbascoside
(0.011%),
isoquercitrin
(0.041%),
astragalin
(0.026%),
kaempferol
(0.009%)
schisandrin
(0.014%)
established.
Moreover,
74
potential
biomarkers
metabolic
pathways
related
identified
multivariate
analysis
metabolite
profiling.
can
regulate
36
markers,
mainly
involving
amino
metabolism,
lipid
metabolism
nucleotide
metabolism.This
a
simple
accurate
quality
WYP.
It
further
enriches
treatment
oligoasthenozoospermia.
provide
theoretical
basis
rational
application
LEV
improves
the
percentage
of
forward-motion
spermatozoon
and
total
sperm
motility
in
patients
with
oligozoospermia
or
asthenospermia
clinical
settings.
However,
mechanism
action
levocarnitine
(LEV)
treatment
spermatogenic
dysfunction
was
unclear.
Based
on
vitro
vivo
experiments,
we
used
Glucosides
Tripterygium
wilfordii
Hook
F
(GTW)
to
construct
a
cell
model
(using
GC-1
spg
cells)
an
animal
rats)
dysfunction.
LY294002
(a
PI3K
pathway
inhibitor)
were
then
administered.
By
assessing
apoptosis
quality
motility,
underlying
explored.
We
found
that
GTW
induced
dysfunction,
ameliorated
GTW-induced
inhibited
improved
count
percentages
PR
(forward
motion)
+
NP
(non-forward
(p
<
.01).
Besides,
morphology
testicular
tissue
LY
groups
superior
group.
can
conclusion
may
operate
via
PI3K/AKT
signalling
pathway,
increases
PI3K,
p-AKT,
BCL-2
protein
mRNA
expression,
so
cells
decrease,
improve.
Journal of Liquid Chromatography & Related Technologies,
Год журнала:
2021,
Номер
44(13-14), С. 682 - 688
Опубликована: Авг. 9, 2021
Cuscuta
chinensis
Lam.
is
a
medicinal
herb
widely
used
in
Chinese
medicine.
However,
it
often
replaced
illegally
with
extracts
of
japonica
Choisy.
Thus,
there
need
to
be
able
distinguish
between
samples
from
these
two
species.
In
this
study,
combination
chemical
HPLC
fingerprinting
and
chemometrics
was
compare
identify
C.
japonica.
The
composition
the
determined
by
fingerprinting.
These
results
were
then
analyzed
chemometrics,
namely
hierarchical
clustering
analysis,
principal
component
both
which
clearly
distinguished
different
herbal
medicines.
addition,
partial
least
squares
discriminant
analysis
model
selected
for
verification,
given
that
showed
stronger
ability
predict
source
new
samples.
Ultimately,
markers
screened
out
using
Variable
Importance
Projection
map.
also
indicated
flavonoids
organic
acids
have
an
important
role
discrimination
japonica,
will
relevance
determining
drug
safety
quality
control
medicines
containing
chinensis.
Pharmacological Research - Modern Chinese Medicine,
Год журнала:
2023,
Номер
6, С. 100224 - 100224
Опубликована: Фев. 1, 2023
Semen
quality
is
an
important
index
for
evaluating
male
fertility
and
reflecting
masculine
reproductive
health.
Infertility
caused
by
oligoasthenozoospermia
(OAS)
has
become
a
significant
problem
that
affects
the
overall
of
life.
The
development
application
safe
effective
drugs
symptomatic
treatment
OAS
are
great
significance
alleviating
population
decline
aging.
A
literature
search
was
performed
in
past
20
years
PubMed,
China
National
Knowledge
Infrastructure
(CNKI),
Wanfang
Data
Platform
(Wanfang)
on
TCM,
expands
dialectical
thinking
TCM
OAS,
systematically
assesses
pharmacological
mechanism
Chinese
herbal
medicine
(CHM)
its
compound
preparations
therapy
this
disease.
theory
guiding
cure
OAS.
CHM
can
be
multi-targeted
to
improve
quantity
sperm,
regulating
symptoms
patients
comprehensive
oligospermia.
This
study
provide
therapeutic
ideas
theoretical
basis
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 5, 2023
Abstract
Background
The
role
of
the
C9orf72
gene
in
sperm
function
and
asthenozoospermia
remains
unclear.
This
study
aimed
to
investigate
whether
expression
was
abnormal
patients
with
its
effects
on
spermatogonia
proliferation.
Methods
Semen
samples
were
collected
from
27
men
31
normal
men.
Immunofluorescence
Western
blotting
employed
detect
protein
semen
samples.
Mouse
(GC-1
spg)
transfected
C9orf72-overexpressing
lentivirus
assessed
for
cell
viability,
apoptosis,
ROS
levels,
mitochondrial
membrane
potential
changes,
colocalization
between
autophagosomes
mitochondria,
glucose
content
energy
metabolism-related
expression.
effect
mitophagy
inhibitor
Mdivi-1
C9orf72-induced
apoptosis
GC-1
spg
cells
investigated.
Results
results
showed
that
significantly
upregulated
asthenozoospermic
negatively
correlated
progressive
motility.
Overexpression
inhibited
proliferation,
increased
elevated
levels
cells.
Enhanced
observed
cells,
as
indicated
by
reduction,
decreased
MitoTracker
Red
CMXRos-labeled
LC-3-labeled
autophagy.
Moreover,
metabolism
impaired
uptake
reduced
GLUT1,
GLUT3,
HK2
LDHA.
partially
reversed
Conclusions
demonstrates
is
overexpressed
overexpression
inhibits
mechanism
which
it
proliferation
may
be
through
promoting
inhibiting
metabolism,
representing
a
regulatory
underlying
asthenozoospermia.
Medicine,
Год журнала:
2023,
Номер
102(47), С. e36196 - e36196
Опубликована: Ноя. 24, 2023
The
study
aimed
to
explore
the
key
targets
and
molecular
mechanisms
of
Dahuang-Tusizi
drug
pair
(DTDP)
in
treatment
diabetes
nephropathy
(DN)
based
on
GEO
database
by
using
network
pharmacology
combined
with
docking
immune
infiltration.
active
components
DTDP
were
screened
Traditional
Chinese
Medicine
Systems
Pharmacology
Swiss
Target
Prediction
database.
differential
genes
DN
retrieved
from
databases.
Next,
intersecting
disease
imported
into
String
for
protein–protein
interactions
analysis,
core
identified
through
topological
analysis.
Gene
Ontology
analysis
Kyoto
Encyclopedia
Genes
Genomes
enrichment
analyses
performed
help
Metascape
gene
set
Subsequently,
was
verify
binding
activity
component
target.
Nephroseq
V5
used
clinical
relevance
genes.
Finally,
Using
CIBERSORT
Algorithm
analyze
Infiltration
Chip.
showed
that
25
ingredients
associated
22
DN.
(Sesamin,
quercetin,
EUPATIN,
matrine,
beta-sitosterol,
isorhamnetin,
etc.)
(JUN,
EGF,
CD44,
FOS,
KDR,
CCL2,
PTGS2,
MMP2)
further
identified.
Enrichment
revealed
signaling
pathways
including
TNF,
MAPK,
IL-17
pathway.
Molecular
results
there
a
strong
affinity
between
targets.
infiltration
found
proportion
macrophages
tissues
significantly
increased.
Our
findings
demonstrated
characteristics
treating
are
“multiple
components,
multiple
pathways.”
We
predicted
may
inhibit
inflammation
related
regulating
genes,
reducing
macrophage
Thus,
inhibiting
inflammatory
response
reduce
glomerular
damage
delay
development
