Theranostics,
Год журнала:
2024,
Номер
14(18), С. 7219 - 7240
Опубликована: Окт. 28, 2024
Rationale:
Sunitinib
is
a
small-molecule
tyrosine
kinase
inhibitor
associated
with
the
side-effect
of
liver
injury.
The
impaired
cell
type
in
and
hepatotoxicity
mechanisms
still
unclear.
Methods:
Spatial
metabolomics,
transmission
electron
microscopy,
immunofluorescence
co-staining,
isolation
bile
duct
cells
sinusoidal
endothelial
(LSECs)
were
used
to
evaluate
zonated
sunitinib.
Farnesoid
X
receptor
(FXR)
conditional
knockout
mice,
metagenomics
analysis,
bacteria
clearance,
bacterial
culture,
Parabacteroides
distasonis
3-oxolithocholic
acid
supplementation
Results:
Phenotype
analysis
found
that
hepatic
autophagy,
apoptosis,
mitochondrial
injury
observed
vivo
or
vitro
after
sunitinib
treatment.
By
using
spatial
metabolomics
LSECs,
drug
toxicity
was
around
portal
vein.
Hepatocytes,
cells,
LSECs
damaged
FXR
inhibition
gut
microbiota
depletion
aggravated
sunitinib-induced
For
diurnal
variation,
enhanced
at
night
compared
day,
participated
circadian
rhythmic
induced
by
Conclusions:
Our
data
suggested
activation
may
be
improve
hepatotoxicity.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 2, 2024
Ginsenosides
and
lipids
are
both
bioactive
ingredients
for
ginseng.
Targeting
these
two
categories
of
components,
this
study
was
designed
to
develop
desorption
electrospray
ionization-mass
spectrometry
imaging
approaches
spatial
metabolomics
strategies,
achieving
the
visualization
differentiation
among
different
parts
Panax
ginseng
root
(e.g.,
rhizome,
main
root,
lateral
fibrous
adventitious
root).
Potential
chemical
markers
were
identified
by
searching
an
in-house
ginsenoside
library
online
Lipid
Maps
database,
together
with
high-resolution
MS2
data
analysis.
Six
ginsenosides
11
diagnostic
differentiate
five
P.
root.
Additionally,
three
20
as
differential
six
positions
High-abundance
malonyl-
oleanolic
acid-ginsenosides
observed
in
rhizome.
These
results
assist
understanding
accumulation
molecules
all
through
ginseng,
which
can
benefit
its
quality
control
rational
use.
Natural Product Communications,
Год журнала:
2024,
Номер
19(6)
Опубликована: Июнь 1, 2024
Humans
are
often
exposed
to
a
variety
of
toxic
substances
(including
some
drugs,
herbs,
environmental
pollutants);
all
these
heterologous
can
result
in
metabolic
changes
organism.
Clarifying
their
details
will
help
understand
the
beneficial
and
effects
those
substances.
Traditional
toxicology
research
tends
focus
on
doses
time
rather
than
mechanisms
detoxification
methods.
Compared
with
other
omics
methods,
metabolomics
has
unique,
dynamic,
phenotypic
characteristics.
Similarly,
link
interaction
between
genes
environment;
it
represents
both
downstream
output
genome
upstream
input
environment,
reflects
interactions
biological
system
stimulation.
It
highlighting
not
only
but
also
closely
related
phenotype
events,
which
is
widely
employed
studies
presently.
Therefore,
strengthening
toxicological
based
better
action
Thus,
present
review
systematically
summarized
discussed
current
application
status
approaches
toxicology,
might
contribute
provide
deeper
understanding
processes
toxicity
caused
by
chemicals
herbal
medicines,
thereby
reducing
occurrence
damage
providing
technical
reference
for
research.
Analytical Sciences,
Год журнала:
2024,
Номер
40(11), С. 2063 - 2073
Опубликована: Авг. 13, 2024
Emodin
is
an
important
anthraquinone
compound
with
good
anti-inflammatory
activity
in
Chinese
traditional
medicine
rhubarb.
Detailed
spatial
distribution
information
bio-tissues
plays
role
revealing
the
pharmacodynamics,
toxicology
and
chemical
mechanism
of
emodin.
Herein,
matrix-assisted
laser
desorption/ionization
time-of-flight
mass
spectrometry
imaging
(MALDI-TOF-MSI)
analytical
method
was
established
to
obtain
on
temporal
changes
emodin
multiple
mouse
tissue
sections
(heart,
liver,
spleen,
lung,
kidney,
brain)
after
intraperitoneal
injection
mice.
The
measurements
were
accomplished
negative
ion
mode
range
m/z
250-285
Da
a
resolution
40
µm.
It
found
that
predominantly
distributed
arteriolar
vascular
region
heart,
capsule
cortex
kidney.
Moreover,
MALDI-TOF-MSI
result
implied
might
be
brain.
These
more
detailed
provides
significant
reference
for
investigating
action
emodin,
which
cannot
obtained
from
conventional
LC-MS
analysis.
trend
results
analysis
agreed
ultra-performance
liquid
chromatography/tandem
(UPLC-MS/MS)
well,
demonstrating
complementarity
reliability
method.
Our
work
provided
label-free
molecular
investigate
precise
various
organs,
prove
great
potential
studying
effective
substances
Theranostics,
Год журнала:
2024,
Номер
14(18), С. 7219 - 7240
Опубликована: Окт. 28, 2024
Rationale:
Sunitinib
is
a
small-molecule
tyrosine
kinase
inhibitor
associated
with
the
side-effect
of
liver
injury.
The
impaired
cell
type
in
and
hepatotoxicity
mechanisms
still
unclear.
Methods:
Spatial
metabolomics,
transmission
electron
microscopy,
immunofluorescence
co-staining,
isolation
bile
duct
cells
sinusoidal
endothelial
(LSECs)
were
used
to
evaluate
zonated
sunitinib.
Farnesoid
X
receptor
(FXR)
conditional
knockout
mice,
metagenomics
analysis,
bacteria
clearance,
bacterial
culture,
Parabacteroides
distasonis
3-oxolithocholic
acid
supplementation
Results:
Phenotype
analysis
found
that
hepatic
autophagy,
apoptosis,
mitochondrial
injury
observed
vivo
or
vitro
after
sunitinib
treatment.
By
using
spatial
metabolomics
LSECs,
drug
toxicity
was
around
portal
vein.
Hepatocytes,
cells,
LSECs
damaged
FXR
inhibition
gut
microbiota
depletion
aggravated
sunitinib-induced
For
diurnal
variation,
enhanced
at
night
compared
day,
participated
circadian
rhythmic
induced
by
Conclusions:
Our
data
suggested
activation
may
be
improve
hepatotoxicity.
