Natural Product Communications,
Год журнала:
2023,
Номер
18(11)
Опубликована: Ноя. 1, 2023
Introduction
Cladonia
rangiformis,
also
known
as
reindeer
lichen,
has
been
used
for
various
remedies
in
folk
medicine.
The
rich
nutritional
value
of
this
lichen
is
reflected
the
fact
that
it
contains
atranorin,
which
proven
to
have
numerous
biological
activities.
Objective
chemical
composition,
antioxidant,
anticholinesterase,
and
antigenotoxic
activities
C.
rangiformis
acetone
extract
were
investigated.
novelty
work
anticholinesterase
activity,
protective
effect
on
human
lymphocytes,
antioxidant
activity
by
cupric-reducing
capacity
(CUPRAC)
method
determined
first
time,
well
gas
chromatography
-
mass
spectrometry
(GC-MS)
profile.
Methods
Chemical
composition
was
carried
out
high-performance
liquid
(HPLC)-diode
array
detector
GC-MS.
Antigenotoxic
evaluated
lymphocytes
cytokinesis-block
micronucleus
assay.
Total
phenolic
content,
DPPH
ABTS
radical
scavenging
capacities,
CUPRAC,
total
reducing
power
spectrophotometrically.
Determination
acetylcholinesterase
performed
Ellman's
colorimetry
statistical
analysis
variance
(One-way
ANOVA)
using
Origin
software
package
version
7.0.
Results
HPLC
identified
3
compounds
with
atranorin
predominant
constituent
(97.2%).
Fumarprotocetraric
atraric
acid
represented
a
much
smaller
mutually
approximate
amount,
0.3
0.5%,
respectively.
Rangiformic
most
abundant
volatile
compound.
Acetone
(2
μg/mL)
decreased
frequency
micronuclei
15.5%
while
radioprotectant
amifostine
reduced
occurrence
11.4%.
On
contrary,
at
concentration
1.0
mg/mL
exhibited
an
activating
cholinesterase
(2.0%),
10.0
showed
weak
inhibitory
(7.3%).
phenol
content
132.71
μg
gallic
equivalents
per
mg
dry
extract.
IC
50
experiment
16.19
11.80
mg/mL.
Cupric
17.81
Trolox
0.45
ascorbic
extract,
Conclusion
In
addition
previously
time
method,
orcinol,
β-orcinol,
lauric
alcohol,
atranol,
rangiformic
GC-MS
method.
results
proved
had
activity.
Reduction
number
classified
promising
substances
source
beneficial
effects
DNA
damage.
Journal of Research in Pharmacy,
Год журнала:
2024,
Номер
28(4)(28(4)), С. 1166 - 1173
Опубликована: Янв. 1, 2024
In
this
study,
we
investigated
the
anti-microbial
and
anti-inflammatory
activities
of
cosmopolite
macrolichens
Usnea
articulata
(L.)
Hoffm.,
Umbilicaria
crustulosa
(Ach.)Lamy
Bryoria
fuscescens
(Gyeln.)Brodo
&
D.Hawksw
hydroalcoholic
extracts
to
contribute
potential
pharmacological
uses
lichens.In
vitro
antimicrobial
ethanol
against
Gram-negative
bacteria
Escherichia
coli,
Gram-positive
Staphylococcus
aureus,
yeast
Candida
albicans
were
presented
using
Broth
microdilution
method.The
most
effective
lichen
extract
gram-positive
S.
aureus
was
U.
with
a
MIC
value
0.125
mg/ml.U.
B.
fuscences
have
similar
anti-fungal
despite
having
values
0.5
mg/ml.The
antiinflammatory
effects
on
Lipopolysaccharide/Interferon-gamma
(LPS/IF-γ)
induced
macrophage-like
cellular
systems
(BV-2
microglia
RAW
264,7
macrophages)
evaluated
by
measuring
P38
mitogen-activated
protein
kinase
phosphorylation
(P38MAPK),
cyclooxygenase
2
(COX-2)
inducible
nitric
oxide
synthase
(NOS2)
mRNA
expression.Especially,
also
attenuated
LPS/IF-γ
increase
in
P38MAPK
phosphorylation,
COX-2,
NOS2
expression
both
cells
without
any
cytotoxicity.According
results
our
suggest
that
mechanism
might
result
from
inhibition
through
reduction
COX-2
expressions.
Journal of Pharmacology and Pharmacotherapeutics,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Objectives
The
current
study
aims
to
establish
the
pharmacognostic
profile
and
evaluate
cytotoxic
potential
of
Parmelia
sulcata
Talyor
in
glioblastoma
cell
line.
Materials
Methods
In
this
experimental
investigation,
evaluation
has
been
conducted
via
standard
procedures,
active
fractions
obtained
through
column
chromatography,
further
identified
by
liquid
chromatography–mass
spectrometry
(LC–MS).
total
antioxidant
capacity
ethanolic
extract
lichen
was
phosphomolybdate
method,
a
reducing
power
assay
determine
its
vitro
potential.
U87-MG
lines
were
cultivated
minimum
essential
medium
supplemented
with
nonessential
amino
acids,
10%
fetal
bovine
serum
maintained
under
controlled
conditions.
3-(4,5-Dimethyl
thiazolyl-2)-2,5-diphenyltetrazolium
bromide
used
test
cytotoxicity
isolated
fraction.
Results
P.
inhabiting
lithophytic
life
form
established.
Morphological
parameters
aided
studying
physical
attributes
lichen,
whereas
microscopic
helped
histological
characteristics.
LC–MS
analysis
purified
confirms
presence
phenolics
lichens
responsible
for
anticancer
activity.
Pharmacological
data
on
revealed
possible
usage
treatment
associated
pathological
Lichen
fraction
(L16–L21)
significantly
(
p
<
0.05)
inhibited
proliferation
U87
cells
dose-dependent
manner.
Conclusion
This
reported
that
shield
promising
activities
lines,
which
marked
reduction
cancer
proliferation.
Further
phytochemical
confirmed
identity
facilitated
inclusion
various
Pharmacopoeias.
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 27, 2024
Abstract
Nature
has
been
a
profound
source
of
medicinal
agents
for
millennia,
with
numerous
modern
drugs
derived
from
natural
sources.
Many
these
discoveries
stem
traditional
medicine
practices.
Plants,
in
particular,
are
rich
secondary
metabolites
like
tannins,
terpenoids,
alkaloids,
and
flavonoids,
known
their
antimicrobial
properties.
Among
these,
lichens,
which
symbiotic
organisms
consisting
fungus
an
alga,
present
significant
potential
medical
exploration.
Studies
have
shown
that
lichen
metabolites,
such
as
depsides,
depsidones,
usnic
acid,
exhibit
activity
against
mycobacteria
Gram-positive
bacteria.
With
the
growing
ineffectiveness
some
conventional
drugs,
there
is
renewed
interest
compounds.
This
study
focuses
on
antioxidant
activities
lichens
Ooty,
Tamil
Nadu,
India.
Extracts
were
analyzed
using
LC-MS
various
assays,
including
phenol
content,
FRAP,
ORAC,
DPPH,
revealing
substantial
activity.
In
vitro
tests
inhibiting
pancreatic
lipase,
α-glucosidase,
α-amylase,
along
silico
predictions
pharmacokinetic
properties,
toxicity
risks,
molecular
interactions,
demonstrated
notable
inhibitory
potential.
research
enhances
our
understanding
bioactive
compounds
temperate
polar
regions
highlights
alternatives
treating
diseases
linked
to
oxidative
stress
metabolic
syndrome.
Journal of Applied and Natural Science,
Год журнала:
2024,
Номер
16(4), С. 1516 - 1529
Опубликована: Дек. 20, 2024
Due
to
the
emergence
of
new
diseases,
drug
discovery
is
an
ever-exciting
field
science.
Researchers
have
shifted
their
focus
from
chemical-based
medicines
drugs
natural
origin
and
structural
synthetic
analogues
solve
problem
developing
antibiotic
resistance
detrimental
impacts
existing
chemical
medications.
Lichens
are
still
one
least
explored
sources
for
discovery.
These
slow-growing
forms
a
rich
source
some
exceptional
secondary
metabolites
known
collectively
as
lichenochemicals.
The
significant
proportion
these
lichenochemicals
bioactive
compounds
with
multiple
pharmacological
properties,
including
antimicrobial,
anticancer,
free
radical
scavenging,
anti-inflammatory,
antidiabetic
neuroprotective
abilities
also
high
in
nutritional
values,
making
this
uncanny
combination
algae
fungi,
together
metabolites,
so
well
renowned
traditional
indigenous
medicine.
Although
only
oral
knowledge
was
available
about
them
earlier,
studies
over
last
few
decades
isolated
identified
unique
medicinal
lichen
biology
most
interesting
promising
areas
research.
More
than
half
species
phytochemicals
yet
be
explored.
However,
lichen-derived
vitamin
D3
vegan
D3.
Likewise,
shown
that
can
significantly
reduce
developed
by
microorganisms.
Advanced
techniques
identification
isolation,
combined
bioinformatics,
predictive
software,
databases,
greatly
aided
understanding
chemistry.
This
review
summarizes
use
lichens
highlight
on
recent
breakthroughs
biology.
Natural Product Communications,
Год журнала:
2023,
Номер
18(11)
Опубликована: Ноя. 1, 2023
Introduction
Cladonia
rangiformis,
also
known
as
reindeer
lichen,
has
been
used
for
various
remedies
in
folk
medicine.
The
rich
nutritional
value
of
this
lichen
is
reflected
the
fact
that
it
contains
atranorin,
which
proven
to
have
numerous
biological
activities.
Objective
chemical
composition,
antioxidant,
anticholinesterase,
and
antigenotoxic
activities
C.
rangiformis
acetone
extract
were
investigated.
novelty
work
anticholinesterase
activity,
protective
effect
on
human
lymphocytes,
antioxidant
activity
by
cupric-reducing
capacity
(CUPRAC)
method
determined
first
time,
well
gas
chromatography
-
mass
spectrometry
(GC-MS)
profile.
Methods
Chemical
composition
was
carried
out
high-performance
liquid
(HPLC)-diode
array
detector
GC-MS.
Antigenotoxic
evaluated
lymphocytes
cytokinesis-block
micronucleus
assay.
Total
phenolic
content,
DPPH
ABTS
radical
scavenging
capacities,
CUPRAC,
total
reducing
power
spectrophotometrically.
Determination
acetylcholinesterase
performed
Ellman's
colorimetry
statistical
analysis
variance
(One-way
ANOVA)
using
Origin
software
package
version
7.0.
Results
HPLC
identified
3
compounds
with
atranorin
predominant
constituent
(97.2%).
Fumarprotocetraric
atraric
acid
represented
a
much
smaller
mutually
approximate
amount,
0.3
0.5%,
respectively.
Rangiformic
most
abundant
volatile
compound.
Acetone
(2
μg/mL)
decreased
frequency
micronuclei
15.5%
while
radioprotectant
amifostine
reduced
occurrence
11.4%.
On
contrary,
at
concentration
1.0
mg/mL
exhibited
an
activating
cholinesterase
(2.0%),
10.0
showed
weak
inhibitory
(7.3%).
phenol
content
132.71
μg
gallic
equivalents
per
mg
dry
extract.
IC
50
experiment
16.19
11.80
mg/mL.
Cupric
17.81
Trolox
0.45
ascorbic
extract,
Conclusion
In
addition
previously
time
method,
orcinol,
β-orcinol,
lauric
alcohol,
atranol,
rangiformic
GC-MS
method.
results
proved
had
activity.
Reduction
number
classified
promising
substances
source
beneficial
effects
DNA
damage.
