Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 2, 2023
Abstract
Purpose
The
incidence
and
mortality
of
hepatocellular
carcinoma
(HCC)
are
increasing.
It
is
urgent
to
develop
more
effective
HCC
biomarkers
for
diagnosis
treatment.
This
project
intends
explore
the
expression
enhancer
zeste
1
polycomb
repressive
complex
2
subunit
(EZH1)
its
mechanism
in
HCC.
Methods
study
integrates
global
microarray
high-throughput
sequencing
datasets,
combined
with
internal
immunohistochemistry,
analyze
prognostic
value
EZH1
Functional
enrichment
analysis
was
conducted
investigate
transcriptional
targets,
which
were
achieved
by
intersecting
over-expressed
genes
co-expressed
genes,
putative
targets.
relationship
between
anticancer
drugs
detected
drug
sensitivity
analysis.
Results
In
this
study,
84
datasets
from
40
platforms
(3926
samples
3428
non-cancerous
liver
tissues)
included
show
high
Immunohistochemistry
159
62
non-HCC
confirmed
a
level.
patients
had
worse
survival
prognoses.
GO
Reactome
revealed
that
metabolism-related
pathways,
including
autophagy,
critical
Interestingly,
as
one
potential
autophagy-related
7
(ATG7)
appeared
above
pathways.
ATG7
positively
correlated
EZH1,
upregulated
HCC,
mediated
poor
prognosis.
Upregulation
found
be
contact
anti-tumor
resistance.
Conclusion
upregulation
can
promote
occurrence
lead
clinical
progression
resistance,
these
effects
may
regulating
ATG7.
Journal of Experimental & Clinical Cancer Research,
Год журнала:
2024,
Номер
43(1)
Опубликована: Янв. 31, 2024
Abstract
Background
Discoidin,
CUB,
and
LCCL
domain-containing
type
I
(DCBLD1)
is
identified
as
an
oncogene
involved
in
multiple
regulation
of
tumor
progression,
but
specific
mechanisms
remain
unclear
cervical
cancer.
Lactate-mediated
lactylation
modulates
protein
function.
Whether
DCBLD1
can
be
modified
by
the
function
are
unknown.
Therefore,
this
study
aims
to
investigate
identify
its
sites.
Herein,
we
elucidated
mechanism
which
modification
stabilizes
protein.
Furthermore,
investigated
overexpression
activating
pentose
phosphate
pathway
(PPP)
promote
progression
Methods
expression
was
examined
human
cancer
cells
adjacent
non-tumorous
tissues
using
quantitative
reverse
transcription-polymerase
chain
reaction,
western
blotting,
immunohistochemistry.
In
vitro
vivo
studies
were
conducted
impact
on
Untargeted
liquid
chromatography-tandem
mass
spectrometry
(LC–MS/MS)
metabolomics
used
characterize
DCBLD1-induced
metabolite
alterations.
Western
blot,
immunofuorescence
transmission
electron
microscopy
performed
detect
degradation
G6PD
autophagy.
Chromatin
immunoprecipitation,
dual
luciferase
reporter
assay
for
detecting
lactate
increases
transcription.
LC–MS/MS
employed
verify
sites
within
Results
We
found
that
increased
expression,
PPP
facilitate
proliferation
metastasis
cells.
primarily
stimulated
upregulating
glucose-6-phosphate
dehydrogenase
(G6PD)
enzyme
activity.
The
enrichment
HIF-1α
promoter
region,
enhancing
mRNA
expression.
Additionally,
lactate-induced
stabilized
a
substrate,
with
predominant
site
at
K172.
inhibited
autophagic
degradation,
progression.
vivo,
6-An
mediated
inhibition
activity,
inhibiting
proliferation.
Conclusions
Our
findings
revealed
novel
post-translational
DCBDL1,
emphasizing
significance
lactylation-driven
DCBDL1-mediated
promoting
Graphical
Schematic
illustration
G6PD-mediated
reprogramming
metabolism
Advances in medical diagnosis, treatment, and care (AMDTC) book series,
Год журнала:
2024,
Номер
unknown, С. 122 - 164
Опубликована: Март 11, 2024
Cancer,
a
critical
health
concern,
necessitates
safer,
more
effective
treatments.
This
chapter
explores
the
anticancer
potential
of
traditional
Thai
plants,
which,
despite
being
underresearched,
show
significant
promise.
It
focuses
on
bioactive
compounds
and
their
efficacy
against
various
cancer
types.
The
categorizes
these
plants
based
active
components
reviews
both
laboratory
animal
studies
to
understand
underlying
mechanisms
involved
in
inhibiting
cell
growth,
inducing
death,
preventing
tumor
blood
vessel
formation.
Clinical
trials
supporting
findings
are
also
highlighted.
Given
improve
or
innovate
treatments,
present
encouraging
prospects.
However,
further
research
is
essential
confirm
safety
approaches.
These
suggest
that
could
lead
development
innovative
drugs,
signaling
advancements
therapy.
Cancer
is
a
daunting
global
health
problem
with
steadily
rising
incidence.
Despite
the
wide
arsenal
of
current
anticancer
therapies,
challenges
such
as
drug
resistance,
tumor
heterogeneity,
poor
targeting,
and
severe
side
effects
often
lead
to
suboptimal
efficacy
patient
outcomes,
highlighting
need
for
innovative
therapies.
Autophagy
modulation
has
emerged
an
attractive
approach
complement
existing
The
dual
role
autophagy
in
cancer
promotion
suppression
inspired
development
new
drugs
therapeutic
strategies
focusing
on
both
inhibition
induction.
promising
results
modulators
preclinical
studies,
lack
selectivity
potency,
toxicity,
pharmacokinetics,
inadequate
targeting
continue
limit
their
successful
clinical
translation.
Many
these
could
be
overcome
using
nanomedicine.
This
review
explores
recent
advancements
nanomedicine
modulation.
Successful
combination
leveraging
nanoparticles
synergy
chemotherapy,
immunotherapy,
phototherapy,
gene
therapy,
other
modalities
are
presented.
Additionally,
nanomaterials
intrinsic
autophagy‐modulating
capabilities,
self‐assembling
inhibitors,
discussed.
Finally,
limitations
currently
trials
discussed,
future
perspectives
designing
implementation
explored.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(23), С. 17056 - 17056
Опубликована: Дек. 2, 2023
Ongoing
research
is
gradually
broadening
the
idea
of
cancer
treatment,
with
attention
being
focused
on
nanoparticles
to
improve
stability,
therapeutic
efficacy,
targeting,
and
other
important
metrics
conventional
drugs
traditional
drug
delivery
methods.
Studies
have
demonstrated
that
carriers
based
biomaterials
(e.g.,
protein
lipids)
inorganic
materials
metal
nanoparticles)
potential
anticancer
effects.
Among
these
carriers,
self-assembled
proteins
peptides,
which
are
highly
biocompatible
easy
standardize
produce,
strong
candidates
for
preparation
drugs.
Breast
(BC)
cervical
(CC)
two
most
common
deadly
cancers
in
women.
These
not
only
threaten
lives
globally
but
also
put
a
heavy
burden
healthcare
system.
Despite
advances
medical
care,
incidence
cancers,
particularly
CC,
almost
entirely
preventable,
continues
rise,
mortality
rate
remains
steady.
Therefore,
there
still
need
in-depth
develop
more
targeted,
efficacious,
safe
therapies.
This
paper
reviews
types
self-assembling
peptides
ferritin,
albumin,
virus-like
particles)
natural
products
soy
paclitaxel)
commonly
used
treatment
BC
CC
describes
can
be
delivered
using
as
siRNAs,
DNA,
plasmids,
mRNAs).
The
mechanisms
(including
self-assembly)
by
act
discussed.
mechanism
action
many
similarities
NF-KB
Wnt).
Thus,
show
CC.
Molecules,
Год журнала:
2024,
Номер
29(11), С. 2641 - 2641
Опубликована: Июнь 3, 2024
Isoliquiritigenin
(ISL)
is
a
chalcone
that
has
shown
great
potential
in
the
treatment
of
cancer.
However,
its
relatively
weak
activity
and
low
water
solubility
limit
clinical
application.
In
this
study,
we
designed
synthesized
21
amino
acid
ester
derivatives
ISL
characterized
compounds
using
Frontiers in Molecular Biosciences,
Год журнала:
2024,
Номер
11
Опубликована: Сен. 4, 2024
Varying
from
other
identified
cell
death
pathways,
cuproptosis
is
a
new
type
of
regulated
characterized
by
excess
Cu
ions,
abnormal
aggregation
lipoylated
proteins
in
TCA
cycle,
loss
Fe-S
cluster
proteins,
upregulation
HSP70,
leading
to
proteotoxic
and
oxidative
stress.
Cuproptosis
highly
concerned
scientific
community
as
the
field
further
develops,
remarkable
progress
has
been
made
verification
mechanism
cuproptosis,
methods
used
detect
have
continuously
improved.
According
characteristic
changes
techniques
based
on
verification,
content,
morphology,
molecular
biology
protein
levels
cuproptosis-related
molecules
biochemical
pathways
enzyme
activity
metabolites
stress,
lipoic
acid,
phosphorylation,
respiration
intensity
subject
research.
In
order
deepen
understanding
detecting
principle
application
common
detection
are
reviewed
categorized
cellular
phenomena
terms
death,
biology,
with
flow
chart.
All
indicating
results
displayed
response
markers
their
advantages
limitations
summaried,
comparison
ferroptosis
performed
this
study.
Our
collection
for
will
provide
great
basis
research
future.
Journal of Gastrointestinal and Liver Diseases,
Год журнала:
2024,
Номер
33(1), С. 44 - 56
Опубликована: Март 30, 2024
Background
and
Aims:
The
incidence
mortality
of
hepatocellular
carcinoma
(HCC)
are
increasing.
It
is
urgent
to
develop
more
effective
HCC
biomarkers
for
diagnosis
treatment.
This
project
intends
verify
the
expression
enhancer
zeste
1
polycomb
repressive
complex
2
subunit
(EZH1)
its
mechanism
in
HCC.
Methods:
study
integrates
global
microarray
high-throughput
sequencing
datasets,
combined
with
internal
immunohistochemistry,
analyze
prognostic
value
EZH1
Functional
enrichment
analysis
was
conducted
investigate
transcriptional
targets,
which
were
achieved
by
intersecting
over-expressed
genes,
co-expressed
genes
putative
targets.
relationship
between
anticancer
drugs
detected
drug
sensitivity
analysis.
Results:
In
this
study,
84
datasets
from
40
platforms
(3,926
samples
3,428
non-cancerous
liver
tissues)
included
show
high
Immunohistochemistry
159
62
non-HCC
confirmed
level.
patients
had
worse
survival
prognoses.
Gene
ontology
Reactome
revealed
that
metabolism-related
pathways,
including
autophagy,
critical
Interestingly,
as
one
potential
autophagy-related
7
(ATG7)
appeared
above
pathways.
ATG7
positively
correlated
EZH1,
upregulated
HCC,
mediated
poor
prognosis.
Upregulation
found
be
contact
anti-tumor
resistance.
Conclusions:
upregulation
can
promote
occurrence
lead
clinical
progression
resistance;
these
effects
may
regulating
ATG7.
