Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Дек. 30, 2024
Triphala
is
a
traditional
Ayurvedic
herbal
formulation
composed
of
three
fruits:
amla
(Phyllanthus
emblica),
bibhitaki
(Terminalia
bellerica),
and
haritaki
chebula).
potent
remedy
that
promotes
digestion,
detoxification,
overall
wellness,
while
also
providing
antioxidant
benefits
through
its
trio
nutrient-rich
fruits.
In
order
to
elucidate
the
individual
contributions
ingredients
from
molecular
perspective,
were
used
for
untargeted
LCMS/MS
analysis.
Fresh
fruits
(PE,
TC,
TB)
collected,
processed
into
coarse
powders,
sequentially
extracted
{hexane,
chloroform,
ethyl
acetate}.
data
analysis
was
performed
on
resultant
metabolites,
with
bioinformatics
tools
employed
pathway
enrichment,
target
prediction,
classification
identified
compounds.
Additionally,
polyphenols
as
key
compounds
potential
health
benefits.
LCMS
extracts
total
10227
features,
resulting
in
2515
annotated
PE
contributing
highest
number
at
1286.
Comparative
revealed
408
non-redundant
74.2%
being
unique
fruits,
underscoring
complementary
phytochemical
profiles.
Pathway
enrichment
highlighted
dominant
phenylpropanoid
biosynthesis
pathways
across
all
extracts,
comprehensive
polyphenol
71
polyphenols,
significant
interactions
predicted
between
gut
microbiota.
five
common
showed
human
targets
related
activity.
These
findings
provide
deeper
understanding
diversity
Triphala,
supporting
use
promoting
health.
Biomedical Chromatography,
Год журнала:
2024,
Номер
38(8)
Опубликована: Июнь 10, 2024
Mongolian
medicine
Sendeng-4
(SD-4)
has
demonstrated
satisfactory
clinical
treatment
outcomes
for
rheumatoid
arthritis
(RA);
nevertheless,
its
bioactive
components
and
the
related
mechanisms
have
not
yet
been
clearly
elucidated.
To
explore
chemical
of
SD-4
in
RA
possible
mechanisms,
an
High
Performance
Liquid
Chromatography-tandem
mass
spectrometry
(HPLC-MS/MS)
method
was
established
to
simultaneously
quantify
main
SD-4,
ultraperformance
LC-Q-Exactive-MS/MS
(UPLC-Q-Exactive-MS/MS)
used
identify
phytochemicals
absorbed
serum.
Then,
using
network
pharmacology
methods,
these
were
constructed
into
a
compound-target
predict
biological
targets
as
well
potential
signaling
pathways.
Transcriptomics
analysis
molecular
docking
validate
results
pharmacology.
Subsequently,
we
complete
Freund's
adjuvant-induced
rat
model
observed
anti-RA
effects
through
assessments
foot
swelling,
ankle
diameter,
score,
morphology,
serum
inflammatory
factors,
histopathological
synovial
tissue.
Specifically,
reverse
transcription-quantitative
polymerase
chain
reaction,
Western
blot,
immunohistochemical
animal
experiments
pathways
phytochemistry,
pharmacology,
transcriptomics.
Tannic
acid,
gallic
corilagin,
crocin
I,
gardenoside,
ferulic
quercetin,
limonin,
rutin,
chlorogenic
verbascoside,
catechin,
epicatechin,
myricetin,
dihydromyricetin
showed
good
linearity
within
their
respective
concentration
ranges
(r
≥
0.9991);
average
recovery
rate
93.77%-109.17%
(relative
standard
deviation
<
2%).
A
total
37
compounds
identified
samples.
Based
on
this,
methods
collected
739
genes
3807
RA.
Network
transcriptomic
that
phosphatidylinositol
3-kinase
(PI3K)-protein
kinase
B
(Akt)
pathway
is
most
relevant
affected
by
In
experiments,
reduced
swelling
scores
rats,
improved
symptoms,
production
factors.
Compared
with
group,
significantly
expression
PI3K-Akt
pathway-related
messenger
RNA
proteins.
addition,
confirmed
results.
This
study
combined
transcriptomics
demonstrate
can
alleviate
regulating
pathway.
research
provides
theoretical
basis
application
offers
effective
strategy
identification
substances
traditional
Chinese
formulas
mechanisms.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 19, 2024
Abstract
Background
Bexarotene,
also
recognized
as
Targretin,
is
categorized
a
retinoid,
type
of
cancer
drug.
Nevertheless,
the
precise
mechanisms
Bexarotene
in
relation
to
colon
remain
unclear.
In
cancer,
SEZ6L2
was
suggested
one
biomarkers
and
targets.
This
study
presents
comprehensive
exploration
role
cancer.
Methods
We
utilized
both
TCGA
data
cohort
Chinese
patients.
meticulous
analysis
478
cases,
expression
levels
were
examined
clinical
characteristics,
staging
parameters,
treatment
outcomes.
Additionally,
we
investigated
pharmacological
impact
on
SEZ6L2,
demonstrating
significant
downregulation
at
mRNA
protein
patients
following
treatment.
Results
consistently
overexpresses
serving
potential
universal
biomarker
with
prognostic
significance,
validated
diverse
cohort.
vitro,
promotes
cell
viability
without
affecting
migration.
inhibits
expression,
correlating
reduced
vitro
vivo.
overexpression
accelerates
declining
survival
rates
an
vivo
context.
Bexarotene’s
efficacy
context-dependent,
effective
parental
cells
but
not
overexpression.
Computational
predictions
suggest
direct
SEZ6L2-Bexarotene
interaction,
warranting
further
experimental
exploration.
Conclusion
The
provides
valuable
insights
into
revealing
its
intricate
relationship
outcomes,
effects.
Context-dependent
therapeutic
responses
emphasize
nuanced
understanding
required
for
SEZ6L2’s
paving
way
targeted
strategies.
Aim
This
study
aims
to
investigate
the
antibacterial,
antifungal,
and
phytochemical
properties
of
methanolic
tuber
extracts
from
Terminalia
chebula.
Additionally,
seeks
assess
in
vitro
anticancer
effects
these
on
an
oral
cancer
cell
line,
as
well
their
antioxidant
anti-inflammatory
activities.
Materials
methods
The
research
involves
examining
antibacterial
antifungal
composition
will
be
analyzed
using
standard
techniques.
tested
while
activities
evaluated
through
appropriate
assays.
Results
demonstrated
that
chebula
exhibit
cytotoxic
line
(KB-1),
reducing
viability
evidenced
by
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide
(MTT)
assay.
A
concentration
30
µg/mL
induced
notable
morphological
changes
observed
under
inverted
fluorescence
microscope.
Antioxidant
assays
showed
a
maximum
absorption
85.3%
with
50
µL
extract,
tests
revealed
76.0%
absorption.
Antimicrobial
activity,
assessed
via
agar-well
diffusion,
indicated
significant
effects,
especially
against
Streptococcus
mutans
Candida
albicans
at
higher
concentrations.
findings
suggest
promising
therapeutic
potential
for
extracts.
Conclusion
may
treat
diseases
caused
studied
organisms.
suggests
tubers
have
commercial
value
due
anti-inflammatory,
antioxidant,
properties.
apoptosis
after
24
hours.
Further
is
needed
understand
active
components
underlying
molecular
mechanisms.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 15, 2024
ABSTRACT
BACKGROUND
The
mitotic
DNA
integrity
checkpoint
signaling
pathway
is
potentially
involved
in
cancers
that
regulate
genomic
stability
where
protein
kinases
play
a
pivotal
role.
16
total
kinase
genes
are
this
pathway:
ATM,
BRSK1,
CDK1,
CDK2,
CHEK1,
CHEK2,
MAP3K20,
NEK11,
PLK1,
PLK2,
PLK3,
PRKDC,
STK33,
TAOK1,
TAOK2,
and
TAOK3.
This
study
aims
to
provide
pan-cancer
profiles
of
the
gene
set
for
potential
prognostic
diagnostic
purposes,
as
well
future
therapeutic
targets
cancer
clinical
setting.
METHODS
Multi-omic
data
was
acquired
genes;
over
9000
samples
33
types
were
analyzed
create
SNV,
CNV,
methylation,
mRNA
expression,
crosstalk,
microRNA
regulation
networks.
RESULTS
SNV
profile
showed
most
these
have
high
mutation
frequency
across
some
types,
such
UCEC
SKCM.
CNVs
associated
with
survival
UCEC,
KIRP,
LGG.
BRCA,
KIRC,
LUAD,
STAD
might
be
affected
by
expression
which
involve
copy
number,
miRNA.
In
addition,
also
cross-talk
known
pathways.
CONCLUSION
may
role
development
and,
adequate
research,
could
developed
biomarkers
diagnosis
prognosis.
However,
further
efforts
necessary
validate
their
value
prognosis
develop
practical
applications
settings.
Nevertheless,
offer
better
overall
understanding
useful
information
reference
regarding
cancer.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Дек. 30, 2024
Triphala
is
a
traditional
Ayurvedic
herbal
formulation
composed
of
three
fruits:
amla
(Phyllanthus
emblica),
bibhitaki
(Terminalia
bellerica),
and
haritaki
chebula).
potent
remedy
that
promotes
digestion,
detoxification,
overall
wellness,
while
also
providing
antioxidant
benefits
through
its
trio
nutrient-rich
fruits.
In
order
to
elucidate
the
individual
contributions
ingredients
from
molecular
perspective,
were
used
for
untargeted
LCMS/MS
analysis.
Fresh
fruits
(PE,
TC,
TB)
collected,
processed
into
coarse
powders,
sequentially
extracted
{hexane,
chloroform,
ethyl
acetate}.
data
analysis
was
performed
on
resultant
metabolites,
with
bioinformatics
tools
employed
pathway
enrichment,
target
prediction,
classification
identified
compounds.
Additionally,
polyphenols
as
key
compounds
potential
health
benefits.
LCMS
extracts
total
10227
features,
resulting
in
2515
annotated
PE
contributing
highest
number
at
1286.
Comparative
revealed
408
non-redundant
74.2%
being
unique
fruits,
underscoring
complementary
phytochemical
profiles.
Pathway
enrichment
highlighted
dominant
phenylpropanoid
biosynthesis
pathways
across
all
extracts,
comprehensive
polyphenol
71
polyphenols,
significant
interactions
predicted
between
gut
microbiota.
five
common
showed
human
targets
related
activity.
These
findings
provide
deeper
understanding
diversity
Triphala,
supporting
use
promoting
health.
