Water decoction of Pericarpium citri reticulatae and Amomi fructus ameliorates alcohol-induced liver disease involved in the modulation of gut microbiota and TLR4/NF-κB pathway DOI Creative Commons
Xingmin Zhang,

Yuechang Huang,

Baizhong Chen

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июнь 20, 2024

Introduction Alcohol consumption alters the diversity and metabolic activities of gut microbiota, leading to intestinal barrier dysfunction contributing development alcoholic liver disease (ALD), which is most prevalent cause advanced diseases. In this study, we investigated protective effects action mechanism an aqueous extraction Pericarpium citri reticulatae Amomi fructus (PFE) on injury. Methods C57BL/6 mice were used establish mouse model injury orally administered 500 1,000 mg/kg/d PFE for 2 weeks. Histopathology, immunohistochemistry, immunofluorescence, Western blotting, qRT-PCR, 16S rDNA amplicon sequencing analyze in treatment alcohol-induced Results Treatment with significantly improved injury, as illustrated by normalization serum alanine aminotransferase, aspartate total triglyceride, cholesterol levels ALD a dose-dependent manner. Administration not only maintained integrity prominently upregulating mucous production tight junction protein expressions but also sensibly reversed dysregulation microecology alcohol-treated mice. Furthermore, reduced hepatic lipopolysaccharide (LPS) attenuated oxidative stress well inflammation related TLR4/NF-κB signaling pathway. The supplementation promoted short-chain fatty acids (SCFAs) Conclusion effectively prevents may regulate LPS-involved gut–liver axis; could provide valuable insights drugs prevent treat ALD.

Язык: Английский

Gut-brain axis mediated by intestinal content microbiota was associated with Zhishi Daozhi decoction on constipation DOI Creative Commons

Leyao Fang,

Xin Yi, Junxi Shen

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Фев. 3, 2025

Background Constipation is a common digestive system disorder, which closely related to the intestinal flora. Zhishi Daozhi decoction (ZDD) traditional Chinese medicine prescription used treat constipation caused by indigestion. This study evaluate efficacy of ZDD in treating and elucidate underlying mechanism. Methods In this study, Kunming mice were administered high-protein diet (HFHPD) loperamide hydrochloride injections induce constipation. The then received varying doses (2.4, 4.7, 9.4 mg/kg) for seven days. Following sampling process, we measured fecal microbial activity. levels 5-hydroxytryptamine (5-HT), vasoactive peptide (VIP), aquaporin-3 (AQP3) quantified using enzyme-linked immunosorbent assay. Changes gut microbiota evaluated through 16S rRNA gene sequencing. Additionally, investigated correlation between specific features 5-HT, VIP, AQP3. Results surface model group (CMM) was rough dry. stool low-dose (CLD), medium-dose (CMD), high-dose (CHD) exhibited smoother texture, resembling that normal (CNM). 5-HT CMM significantly lower than CNM, CLD, CHD. VIP CMD other four groups, AQP3 showed decreasing trend. activity higher groups. Diversity analysis indicated CHD treatments more effective restoring structure. Potential pathogenic bacteria, including Clostridium , Aerococcus Jeotgalicoccus Staphylococcus enriched CMM. contrast, beneficial bacteria such as Faecalibacterium Bacillaceae Bacillus prevalent CMD, Correlation revealed Streptococcus Enterococcus positively correlated with while Succinivibrio negative 5-HT. Conclusions induced HFHPD disrupts structure microbiota. appears alleviate constipation, potentially mechanisms linked brain-gut axis its interaction Among treatment medium dose demonstrated most results.

Язык: Английский

Процитировано

0
Exploring Bioactive Constituents and Pharmacological Effects of Scutellaria baicalensis Georgi: A Review DOI Creative Commons
Yan Liu,

Zhixu Gao,

Yintao Zhao

и другие.

Natural Product Communications, Год журнала: 2024, Номер 19(8)

Опубликована: Авг. 1, 2024

Scutellaria baicalensis, commonly known as Chinese Skullcap, is a traditional herb with long history of cultural use in medicines. This review focuses on comprehensive summary the morphological description, bioactive compounds, drug-herb interactions, and pharmacological activities well its therapeutic applications. A wide-ranging search using databases such PubMed, Scopus, Google Scholar, advanced Web was conducted for data collection from prior studies. The plant root contains major active constituents including flavonoids flavo-glycosides wogonin, baicalein, baicalin, oroxylin A, scutellarein, norwogonin. These compounds have demonstrated variety activities, combating inflammation, oxidative stress, cancer, neuronal disorders, liver disorders. Skullcap represents promising avenue potential pharmacotherapeutic formulation advancement, emphasizing need further scientific exploration clinical trials.

Язык: Английский

Процитировано

1
A herbal pair of Scutellaria barbata D. Don and Scleromitrion diffusum (Willd.) R.J. Wang induced ferroptosis in ovarian cancer A2780 cells via inducing heme catabolism and ferritinophagy DOI
Zhen Wang, Min Liu, Guangxing Li

и другие.

Journal of Integrative Medicine, Год журнала: 2024, Номер 22(6), С. 665 - 682

Опубликована: Окт. 18, 2024

Язык: Английский

Процитировано

1
Protective effects of Bidens pilosa alleviates against alcohol – induced hepatic steatosis in Rats: In vivo studies and in silico analysis DOI Creative Commons
Melva Silitonga, Hudson Sidabutar, Hendro Pranoto

и другие.

Pharmacological Research - Modern Chinese Medicine, Год журнала: 2024, Номер 13, С. 100546 - 100546

Опубликована: Ноя. 16, 2024

Язык: Английский

Процитировано

1
Water decoction of Pericarpium citri reticulatae and Amomi fructus ameliorates alcohol-induced liver disease involved in the modulation of gut microbiota and TLR4/NF-κB pathway DOI Creative Commons
Xingmin Zhang,

Yuechang Huang,

Baizhong Chen

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июнь 20, 2024

Introduction Alcohol consumption alters the diversity and metabolic activities of gut microbiota, leading to intestinal barrier dysfunction contributing development alcoholic liver disease (ALD), which is most prevalent cause advanced diseases. In this study, we investigated protective effects action mechanism an aqueous extraction Pericarpium citri reticulatae Amomi fructus (PFE) on injury. Methods C57BL/6 mice were used establish mouse model injury orally administered 500 1,000 mg/kg/d PFE for 2 weeks. Histopathology, immunohistochemistry, immunofluorescence, Western blotting, qRT-PCR, 16S rDNA amplicon sequencing analyze in treatment alcohol-induced Results Treatment with significantly improved injury, as illustrated by normalization serum alanine aminotransferase, aspartate total triglyceride, cholesterol levels ALD a dose-dependent manner. Administration not only maintained integrity prominently upregulating mucous production tight junction protein expressions but also sensibly reversed dysregulation microecology alcohol-treated mice. Furthermore, reduced hepatic lipopolysaccharide (LPS) attenuated oxidative stress well inflammation related TLR4/NF-κB signaling pathway. The supplementation promoted short-chain fatty acids (SCFAs) Conclusion effectively prevents may regulate LPS-involved gut–liver axis; could provide valuable insights drugs prevent treat ALD.

Язык: Английский

Процитировано

0