Integrating Transcriptomics, Metabolomics, and Microbiomics to Explore the Mechanism of Action of Bran-Fried Atractylodes Lancea Rhizome Polysaccharide in Ameliorating the Enhanced Pharmacological Effects of Dextran Sodium Sulfate-Induced Colitis

Опубликована: Янв. 1, 2025

Язык: Английский

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Cucumaria frondosa intestines and ovum hydrolysates intervention ameliorates the symptoms of dextran sulfate sodium‐induced colitis by modulating gut microbiota and its metabolites

Journal of Food Science, Год журнала: 2025, Номер 90(3)

Опубликована: Март 1, 2025

Colitis, a troublesome inflammatory disease that significantly impacts daily life, has garnered considerable attention in recent times. Protolysates play crucial role the treatment of colitis, and intestines ovum Cucumaria frondosa represent readily available source these hydrolysates. However, effects C. hydrolysates (CFHs) on colitis have not been thoroughly investigated. We initially examined molecular weight distribution CFHs found fraction molecules with less than 1000 Da accounted for 86.98%, indicating hydrolysis primarily produced oligopeptides. Subsequently, we employed dextran sulfate sodium-induced experimental model to assess therapeutic potential CFHs. The findings indicated preventive administration dramatically attenuated pathological manifestations associated mice, including loss, colon shortening, tissue damage. Furthermore, suppressed secretion pro-inflammatory cytokines IL-6, TNF-α, IL-1β, as well MPO tissue. Metagenomic sequencing demonstrated could restore balance dysregulated gut microbiota by reinforcing Bacteroidota suppressing Verrucomicrobia populations, impacting various microbial functions. Metabolomic analyses further revealed exhibited more efficacious modulatory effect DSS-induced metabolic abnormalities, amino acid biosynthesis, linoleic metabolism, dopaminergic synapses. In conclusion, showed promise alleviating laying groundwork development application functional food relief.

Язык: Английский

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Cordyceps militaris extract and cordycepin ameliorate LPS-challenged colonic damage in piglets by modulating the microbiota and metabolite profiles

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 10, 2025

Introduction Cordyceps militaris extract (CME) and cordycepin (CPN) are biomolecules with a wide range of biological activities, including anti-inflammatory, antioxidant anti-tumour effects. The research objective wasto investigate the influences CME CPN on colonic morphology, microbiota composition metabolomics in lipopolysaccharide (LPS)-challenged piglets. Methods Twenty-four weaned castrated piglets were randomly divided into four groups: control group (fed basal diets), LPS CPN-LPS (basal diets + 60 mg/kg cordycepin), CME-LPS C. militari sextract). On 21st day, LPS, CPN-LPS, groups received an injection 100 μg/kg BW while was given sterile saline. Results findings demonstrated that or attenuated intestinal morphology damage LPS-challenged alleviated dysbiosis metabolic disorders under by enriching serum protein levels, regulating inflammatory cytokine secretion altering microbial composition. Colonic analysis improved relative abundance Acidobacteriota inhibited Faecalibacterium , promoted Prevotella Lachnospiraceae NK4A136 group. Meanwhile, alleviation is achieved through modulation pathways linked to tryptophan metabolism, biosynthesis amino acids butanoate metabolism. Discussion Conclusively, our preliminary reveal could serve as beneficial dietary supplement alleviate gut diseases weaning

Язык: Английский

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Rhodiola crenulata polysaccharide alleviates dextran sulfate sodium-induced ulcerative colitis in mice by repairing the intestinal barrier and regulating the intestinal microecology

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 26, 2025

Polysaccharides, vital biological macromolecules ubiquitous in organisms, have garnered attention as potential therapeutic candidates for ulcerative colitis (UC). However, the of Rhodiola crenulata polysaccharides (RCP) UC remains largely unexplored. The RCP was prepared by boiling water extraction, 80% alcohol precipitation, membrane separation, and D101 macroporous resin purification. monosaccharide composition (Mw = 67.848 kDa) includes mannose, rhamnose, glucuronic acid, galacturonic glucose, galactose, arabinose, with a molar ratio 0.22:1:0.07:7.03:2.88:0.64:4.12. In vivo experiments shown that can improve DSS induced weight loss mice, decrease disease activity index (DAI), alleviate histopathological changes colon tissue, suppress levels pro-inflammatory cytokine IL-6 MPO activity. Immunohistochemical results showed essential tight junction proteins such occludin, claudin1, ZO-1 were upregulated, improving integrity intestinal barrier. Importantly, regulated abundance microbiota reducing Firmicutes-to-Bacteroidetes (F/B), increasing beneficial bacteria Muribaculaceae Bifidobacterium , decreasing harmful including Erysipelotrichaceae, Faecalibaculum Lachnospiraceae_unclassified Parabacteroides Ruminiclostridium_9 . Additionally, it enhanced restoration acetic propionic isovaleric valeric acid to maintain SCFA levels, thereby restoring microecology. Therefore, has excellent effects on is worthy further drug development clinical treatment.

Язык: Английский

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Tea residue protein-derived oligopeptides attenuate DSS-induced acute colitis complicated with hepatic injury in C57BL/6J mice by regulating the gut-microbiome-liver axis

Phytomedicine, Год журнала: 2025, Номер unknown, С. 156792 - 156792

Опубликована: Апрель 1, 2025

Язык: Английский

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Traditional Chinese Medicine Formulas-Based Interventions on Colorectal Carcinoma Prevention: The Efficacies, Mechanisms and Advantages

Journal of Ethnopharmacology, Год журнала: 2024, Номер unknown, С. 119008 - 119008

Опубликована: Окт. 1, 2024

Язык: Английский

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Scutellarin alleviated ulcerative colitis through gut microbiota-mediated cAMP/PKA/NF-κB pathway

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 735, С. 150837 - 150837

Опубликована: Окт. 15, 2024

Язык: Английский

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Pharmacodynamic and pharmacokinetic study of Shaoyao Gancao decoction for repairing intestinal barrier damage in ulcerative colitis

Molecular Immunology, Год журнала: 2024, Номер 175, С. 132 - 142

Опубликована: Окт. 5, 2024

Язык: Английский

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Exploring the promising potential of alcohol extract from the aerial part of dill in ameliorating DSS-induced ulcerative colitis in mice

Journal of Ethnopharmacology, Год журнала: 2024, Номер 340, С. 119237 - 119237

Опубликована: Дек. 11, 2024

Язык: Английский

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Fecal microbiota transplantation for the treatment of intestinal and extra‐intestinal diseases: Mechanism basis, clinical application, and potential prospect

Bioengineering & Translational Medicine, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 7, 2024

To review the theoretical basis and therapeutic effects of fecal microbiota transplantation (FMT) in various diseases animal experiments clinical studies, as well limitations current standards FMT application. PubMed Web Science databases were searched for articles published only English between 1975 2023 on reliable results treatment FMT. The properties gut its interactions with host metabolism are critical to human health, microbiome disturbance is closely associated intestinal extra-intestinal diseases. Therefore, tools targeting modulation have attracted increasing attention, among which represents most widely studied intervention strategy. This gathered summarized application diseases, metabolic hypertension, cancer, nervous system arthritis, elaborated beneficial that can be achieved by altering mechanisms action. In addition, potential risks side approach discussed, efforts standardize development Through a systemic outcome mechanism we aimed provide construction an optimized framework, so better exert prospects.

Язык: Английский

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