Yunvjian decoction attenuates lipopolysaccharide-induced acute lung injury by inhibiting NF-κB/NLRP3 pathway and pyroptosis

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Янв. 24, 2025

Introduction Yunvjian (YNJ) decoction, a classic traditional Chinese medicine prescription for inflammatory diseases, has demonstrated good therapeutic effects in the clinical treatment of pneumonia. The aim this study was to clarify effective ingredients and mechanism action YNJ on lipopolysaccharide (LPS)-induced acute lung injury (ALI). Methods were evaluated mouse model LPS-induced ALI LPS-treated MLE-12 murine epithelial cells RAW264.7 macrophages vitro . these systems studied using RNA sequencing, immunohistochemical analysis, immunoblotting, immunofluorescence, ELISA, polymerase chain reaction assays. Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry applied identify absorbed components YNJ. Results attenuated pulmonary damage mice, as evidenced by reduced protein content bronchoalveolar lavage fluid, decreased wet/dry weight ratio, improved respiratory function. Analysis pneumonia-related samples from patients Gene Expression Omnibus dataset GSE40012 indicated that NOD-like receptor 3 (NLRP3)-mediated pyroptosis primary ALI. phosphorylation nuclear factor-kappa B (NF-κB) expression levels NLRP3, apoptosis-associated speck-like containing CARD (ASC), cleaved caspase-1, interleukin-1β (IL-1β) vivo Administration YNJ-containing serum increased cell viability malondialdehyde reactive oxidative species contents LPS-stimulated cells. also secretion tumor necrosis factor-α, IL-6, IL-1β macrophages, promoted macrophage polarization toward an M2 phenotype. A total 23 identified serum. Among those, network analysis experiments diosgenin, timosaponin BII, mangiferin are anti-inflammatory active substances. Conclusion attenuates mice inhibiting via suppression NF-κB/NLRP3 pathway. Our findings provide novel insights into

Язык: Английский

Inhibition of endoplasmic reticulum stress and excessive autophagy by Jiedu Tongluo Tiaogan Formula via a CaMKKβ/AMPK pathway contributes to protect pancreatic β-cells

Journal of Ethnopharmacology, Год журнала: 2024, Номер 333, С. 118440 - 118440

Опубликована: Июнь 15, 2024

Jiedu Tongluo Tiaogan Formula (JTTF), a traditional Chinese herbal decoction, exhibits the potential to treat type 2 diabetes mellitus (T2DM) by inhibiting endoplasmic reticulum stress (ERS) and excessive autophagy, which are risk factors for abnormal development progression of β cells.

Язык: Английский