Pharmaceuticals, Год журнала: 2024, Номер 17(12), С. 1675 - 1675

Опубликована: Дек. 12, 2024

Objectives: Traditional Chinese Medicine (TCM) is recognized for its complex composition and multiple therapeutic targets. However, current pharmacological research often concentrates on extracts or individual components. The former approach faces numerous challenges, whereas the latter oversimplifies disregards synergistic effects among TCM This study aims to investigate scientific validity of focusing active constituent in efficacy research, using Pueraria lobata (P. lobata) as a case study. Methods: Through spectrum-effect correlation analysis, network pharmacology, molecular docking, five ingredients P. were identified: puerarin, formononetin, tuberosin, 4′,7-dihdroxy-3′-methoxyisoflavone, Daidzein-4,7-diglucoside. These combined form an constituent, which was subsequently tested vitro vivo. Results: In vitro, exhibited superior enhancing glucose consumption glycogen synthesis compared both extract vivo experiments demonstrated that medium high doses significantly more effective than extract, with comparable those metformin reducing blood sugar levels. Conclusions: effectively improves T2DM by lowering levels, promoting synthesis, modulating glycolipid metabolism. Both studies indicate it outperformed establishes feasibility utilizing focus investigating TCM, thereby offering novel insights new paradigm future investigations.

Язык: Английский