Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus Aspergillus sp. Strain SF-7367
Molecules,
Год журнала:
2025,
Номер
30(2), С. 294 - 294
Опубликована: Янв. 13, 2025
Inflammation
has
always
been
considered
a
trigger
or
consequence
of
neurodegenerative
diseases,
and
the
inhibition
inflammation
in
central
nervous
system
can
effectively
protect
nerve
cells.
Several
studies
have
indicated
that
various
natural
products
inhibit
neuroinflammation.
Among
these,
Antarctic
fungal
metabolites
pharmacological
activities
developmental
value.
Therefore,
this
study
aimed
to
evaluate
anti-neuroinflammatory
activity
an
fungus
belonging
Aspergillus
(strain
SF-7367).
Secondary
SF-7367
were
isolated
using
high-performance
liquid
chromatography
followed
by
validation
their
anti-inflammatory
effects
lipopolysaccharide-stimulated
BV2
microglia
RAW264.7
macrophages.
Chemical
analysis
from
strain
revealed
five
known
compounds:
epideoxybrevianamide
E
(1),
brevianamide
V/W
(2),
K
(3),
Q
(4),
R
(5).
these
compounds,
showed
significant
against
both
cell
types.
Results
Western
blotting
molecular
docking
could
regulate
activation
nuclear
factor
kappa-light-chain-enhancer
activated
B
(NF-κB)
signaling.
This
indicates
present
sp.
SF-7367)
inflammatory
responses
reducing
lipopolysaccharide-induced
translocation
NF-κB
(p65).
These
findings
suggest
are
candidate
agents
for
treating
diseases.
Язык: Английский
Uncovering the antidepressant active ingredients and related molecular mechanisms of Xiaoyao pill using integrated pharmacological strategy
Journal of Chromatography B,
Год журнала:
2025,
Номер
1255, С. 124502 - 124502
Опубликована: Фев. 7, 2025
To
investigate
antidepressant
active
ingredients
of
XYP
(Xiaoyao
Pill),
while
predicting
its
primary
pharmacodynamic
material
basis
and
underlying
mechanisms
action.
UPLC-Q-TOF-MS/MS
was
used
to
identify
the
XYP.
In
addition,
based
on
analysis
components,
network
pharmacology
molecular
docking
were
potential
therapeutic
targets
possible
signaling
pathways
in
treatment
depression.
A
total
102
chemical
10
prototype
components
16
metabolites
absorbed
brain
identified
Network
showed
that
these
compounds
shared
420
common
with
depression,
TP53,
EGFR,
PTGS2,
ESR1,
PPARG
other
68
considered
as
core
targets,
mainly
enriched
PI3K-Akt
MAPK
pathways.
GO
unveiled
associated
apoptosis
inflammatory
response.
Molecular
revealed
paeoniflorin,
liquiritin,
atractylenolide
III
found
have
highest
binding
energy
ESR1
PPARG.
These
findings
suggested
may
exert
effects
through
III,
saikosaponin
D,
formononetin,
affecting
PIK3/AKT
pathway.
This
lays
foundation
for
research
quality
standards
clinical
rational
application
traditional
Chinese
medicine
formulas.
Язык: Английский
mPFC DCC coupling with CaMKII+ neuronal excitation participates in behavioral despair in male mice
Translational Psychiatry,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 14, 2025
A
longed
lack
of
control
over
harmful
stimuli
can
lead
to
learned
helplessness
(LH),
a
significant
factor
in
depression.
However,
the
cellular
and
molecular
mechanisms
underlying
LH,
eventually
behavioral
despair,
remain
largely
unknown.
The
deleted
colorectal
cancer
(dcc)
gene
is
associated
with
risk
therapeutic
potential
regulation
mechanism
DCC
despair
are
still
uncertain.
In
this
study,
we
showed
that
depressive
stimulators,
including
lipopolysaccharide,
unpredictable
chronic
mild
stress,
triggered
an
elevation
expression
medial
prefrontal
cortex
(mPFC).
Additionally,
elevated
mPFC
was
crucial
inducing
as
evidenced
by
induction
normal
mice
exacerbation
LH
upon
overexpression.
By
contrast,
neutralizing
activity
ameliorated
LH-induced
despair.
Importantly,
elucidated
pathological
attributable
excessive
excitation
CaMKII+
neurons
manner
dependent
on
calpain-mediated
degradation
SCOP
aberrant
phosphorylation
ERK
signaling
pathway.
addition,
increase
led
decreased
excitability
threshold
mPFC,
which
supported
observation
ligand
netrin
1
increased
frequency
action
firing
spontaneous
excitatory
postsynaptic
currents
neurons.
conclusion,
our
data
indicate
triggers
activation
calpain-SCOP/ERK
promote
expression,
represents
target
for
treatment
male
mice.
Язык: Английский
The neuroscientific basis of post-traumatic stress disorder (PTSD): From brain to treatment
Progress in brain research,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Single‐Nucleus RNA Sequencing Reveals That Gabra6+ Neurons in Prefrontal Cortex Promote the Progression of PTSD After Shockwave‐Induced TBI
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 12, 2024
Abstract
Shockwave‐induced
traumatic
brain
injury
(TBI)
results
in
the
onset
of
post‐traumatic
stress
disorder
(PTSD),
triggered
either
by
TBI
itself
or
other
stressors.
However,
interplay
and
underlying
mechanisms
how
these
factors
synergistically
induce
PTSD
remain
inadequately
elucidated.
Here,
mice
(induced
biological
shock
tube
blast
injury)
single
prolonged
method)
groups
both
displayed
symptoms
behaviors,
with
TBI+PTSD
(composite
model)
group
exhibiting
more
severe
manifestations.
The
result
snRNA‐seq
demonstrated
a
noticeable
increase
population
Gabra6
+
neurons
prefrontal
cortex
region
group.
Knocking
down
cortical
mitigated
PTSD‐related
behavioral
outcomes.
Mechanistically,
Smad3/4
complex
activation
led
to
upregulation
expression
neurons.
Interaction
Homer1
activated
downstream
cAMP
signaling
pathways.
KO‐Nestin
show
reduced
susceptibility
PTSD.
Subsequently,
efficacy
monoclonal
antibody
intervention
at
218
site
ameliorating
development
is
verified.
This
study
suggests
that
stressors
act
as
independent
components
development,
pivotal
facilitating
formation.
Strategies
geared
toward
minimizing
exposure
singular
combined
may
effectively
diminish
risk
developing
Язык: Английский