Eleutheroside B alleviates oxidative stress and neuroinflammation by inhibiting the JAK2/STAT3 signaling pathway in a rat high altitude cerebral edema model

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 15, 2024

Background High altitude cerebral edema (HACE) is a condition where the central nervous system experiences severe impairment as result of sudden oxygen deprivation at high elevations. At present, effective measures for preventing and treating this are still lacking. Eleutheroside B (EB), primary natural active compound found in senticosus , has demonstrated various biological functions. It also shown significant potential addressing acute mountain sickness neurological disorders. However, additional investigation required to explore protective effects its underlying mechanisms EB on HACE. Methods The male rats received pre-treatment with either vehicle, 100 mg/kg or 50 mg/kg, Dexamethasone 4 coumermycin A1 μg/kg. To simulate hypobaric hypoxia environment plateau 6,000 m, chamber was utilized. therapeutic were assessed through measurements brain water content, histopathological observation, evaluation oxidative stress inflammatory factors using immunofluorescence ELISA. Furthermore, molecular docking, dynamics simulation Western blot employed clarify mechanism. Through these analyses, mechanism by which HACE identified. Results Pre-treatment effect against effectively reducing down-regulating HIF-1α AQP4 protein expression induced reversing pathological changes tissue neuron damage. Compared group treated alone, pre-treated showed reduction levels ROS MDA, well an increase GSH. In addition, led decrease IL-1β, IL-6, TNF-α. Molecular docking simulations indicated that strong binding affinity JAK2/STAT3 signaling pathway. further confirmed significantly downregulated related proteins rats. Additionally, A1, agonist JAK2, reversed anti-oxidative neuroinflammation EB. Conclusion exerts antioxidant anti-neuroinflammatory inhibiting pathway rat model.

Язык: Английский

Brassica rapa L. crude polysaccharide meditated synbiotic fermented whey beverage ameliorates hypobaric and hypoxia induced intestinal damage

Food & Function, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Hypobaric hypoxia causes oxidative stress and inflammatory responses disrupts the gut microbiome metabolome. In this study, we developed a synbiotic fermented whey beverage, combining kefir

Язык: Английский